... understand the mode of binding
and interference with enzyme catalysis.
As the inactivation of any enzyme involved in a
metabolic chain will render the whole chain inoperat-
ive, the inactivation of any ... of the six proteins
involved in pyrimidine de novo synthesis should result
in the same profound effect on the pyrimidine nuc-
leotide pools in C. albicans. How...
... Zoom, the
Netherlands). Ca
2+
binding of the enzyme was studied at
25 °C by monitoring the intrinsic tryptophan fluorescence
of the enzyme (2.2 lm )in1 0mm sodium acetate pH 5.0 as
a function of CaCl
2
concentration ... by recording CD spectra in the presence of increas-
ing concentrations of CaCl
2
(0–10 mm).
Thermal stability
Thermal stability was studied by incubating...
... 2007)
doi:10.1111/j.1742-4658.2007.05818.x
The biosynthesis of lovastatin in Aspergillus terreus requires two mega-
synthases. The lovastatin nonaketide synthase, LovB, synthesizes the inter-
mediate dihydromonacolin L using nine ... 15-fold increase in the yield of the
soluble protein. Pantetheinylation of the ACP in ACP-
CON didomain was verified using MALDI-T...
... 4A). In contrast, 10–15% of the Kv2.1 material
did not bind to Mono-S. The finding of Kv2.1 in the
Mono-S flow-through fractions was independent of the
quantities of loading materials, suggesting there ... application of increasing NaCl in a lin-
ear gradient as indicated by the dotted lines on the chromatogram.
Shaded area represents the locations of fractions s...
... using
crotonyl-CoA as a substrate [15]. The gene encoding
this bifunctional protein was named AUH (‘AU bind-
ing homolog of enoyl-CoA hydratase’). The RNA-
binding activity of the human protein ... For the
kinetic characterization of AUH described in the work
at hand, AUH was overproduced in Escherichia coli as
a maltose binding protein fusion (MBP-AUH). The
complete A...
... subtilis
protein) in the direct vicinity of the FMN binding site which
is not present in the other members of the family.
Thus, under structural aspects, Idi-2 appears as a fairly
distant relative of the ... E153 in close proximity
to the FMN binding site suggests that the substrates could
be bound in close proximity to the isoalloxazine moiety. In
the absenc...
... 208-1102. In
both digestion products, the cleavage site protected by
the presence of ubiquitin was Ser341, located in the
‘fingers’ region of the catalytic core domain involved
in the recognition of the ... oligomeriza-
tion events. Deletion of the N- and C-terminal
domains of USP7 affected the activity of the enzyme,
with the C-terminus having a major i...
... to the
glycosaminoglycan.
For annexin A5, a model of heparin binding has
been put forward in which recognition and binding of
heparin occurs on the concave side of the protein via
the two binding ... the
calcium-binding sites on the convex domain. Assuming
the presence of further heparin-binding sites elsewhere
on the protein surface, annexin B1 can remain bound
to...
... make them easy to recognize. They
contain a fused cytochrome b5 as an N-terminal
domain and three histidine boxes in the desaturase
(C-terminal) domain. The third histidine box has the
QX
2
HH instead ... of front-end desaturases are
present in each of these trypanosomatid proteins,
including the three highly conserved histidine boxes
and the cytochrome b5 N-terminal domain...
... as lmol of leucine,
alanine or tyrosine released per min per mg of protein. The
kinetic parameters k
cat
and K
m
were obtained from Hanes–
Woolf plots. To examine whether rHlSCP1 has the ability ... Tokyo, Japan). The densities of stained
bands were measured using image j 1.36b (National Insti-
tutes of Health) and the ratio of the density divided by that
of the contro...