Báo cáo khoa học: Biochemical characterization of the minimal polyketide synthase domains in the lovastatin nonaketide synthase LovB pot

Báo cáo khoa học: Biochemical characterization of recombinant dihydroorotate dehydrogenase from the opportunistic pathogenic yeast Candida albicans pot

Báo cáo khoa học: Biochemical characterization of recombinant dihydroorotate dehydrogenase from the opportunistic pathogenic yeast Candida albicans pot

... understand the mode of binding and interference with enzyme catalysis. As the inactivation of any enzyme involved in a metabolic chain will render the whole chain inoperat- ive, the inactivation of any ... of the six proteins involved in pyrimidine de novo synthesis should result in the same profound effect on the pyrimidine nuc- leotide pools in C. albicans. How...
Ngày tải lên : 07/03/2014, 12:20
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Báo cáo khoa học: Biochemical characterization of the major sorghum grain peroxidase pptx

Báo cáo khoa học: Biochemical characterization of the major sorghum grain peroxidase pptx

... Zoom, the Netherlands). Ca 2+ binding of the enzyme was studied at 25 °C by monitoring the intrinsic tryptophan fluorescence of the enzyme (2.2 lm )in1 0mm sodium acetate pH 5.0 as a function of CaCl 2 concentration ... by recording CD spectra in the presence of increas- ing concentrations of CaCl 2 (0–10 mm). Thermal stability Thermal stability was studied by incubating...
Ngày tải lên : 16/03/2014, 13:20
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Báo cáo khoa học: Biochemical characterization of the minimal polyketide synthase domains in the lovastatin nonaketide synthase LovB pot

Báo cáo khoa học: Biochemical characterization of the minimal polyketide synthase domains in the lovastatin nonaketide synthase LovB pot

... 2007) doi:10.1111/j.1742-4658.2007.05818.x The biosynthesis of lovastatin in Aspergillus terreus requires two mega- synthases. The lovastatin nonaketide synthase, LovB, synthesizes the inter- mediate dihydromonacolin L using nine ... 15-fold increase in the yield of the soluble protein. Pantetheinylation of the ACP in ACP- CON didomain was verified using MALDI-T...
Ngày tải lên : 30/03/2014, 08:20
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Báo cáo khoa học: Biochemical characterization of the native Kv2.1 potassium channel ppt

Báo cáo khoa học: Biochemical characterization of the native Kv2.1 potassium channel ppt

... 4A). In contrast, 10–15% of the Kv2.1 material did not bind to Mono-S. The finding of Kv2.1 in the Mono-S flow-through fractions was independent of the quantities of loading materials, suggesting there ... application of increasing NaCl in a lin- ear gradient as indicated by the dotted lines on the chromatogram. Shaded area represents the locations of fractions s...
Ngày tải lên : 30/03/2014, 20:20
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Tài liệu Báo cáo khoa học: Biochemical characterization of human 3-methylglutaconyl-CoA hydratase and its role in leucine metabolism docx

Tài liệu Báo cáo khoa học: Biochemical characterization of human 3-methylglutaconyl-CoA hydratase and its role in leucine metabolism docx

... using crotonyl-CoA as a substrate [15]. The gene encoding this bifunctional protein was named AUH (‘AU bind- ing homolog of enoyl-CoA hydratase’). The RNA- binding activity of the human protein ... For the kinetic characterization of AUH described in the work at hand, AUH was overproduced in Escherichia coli as a maltose binding protein fusion (MBP-AUH). The complete A...
Ngày tải lên : 19/02/2014, 07:20
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Tài liệu Báo cáo khoa học: Biochemical characterization of Bacillus subtilis type II isopentenyl diphosphate isomerase, and phylogenetic distribution of isoprenoid biosynthesis pathways doc

Tài liệu Báo cáo khoa học: Biochemical characterization of Bacillus subtilis type II isopentenyl diphosphate isomerase, and phylogenetic distribution of isoprenoid biosynthesis pathways doc

... subtilis protein) in the direct vicinity of the FMN binding site which is not present in the other members of the family. Thus, under structural aspects, Idi-2 appears as a fairly distant relative of the ... E153 in close proximity to the FMN binding site suggests that the substrates could be bound in close proximity to the isoalloxazine moiety. In the absenc...
Ngày tải lên : 19/02/2014, 13:20
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Báo cáo khoa học: Biochemical characterization of USP7 reveals post-translational modification sites and structural requirements for substrate processing and subcellular localization pptx

Báo cáo khoa học: Biochemical characterization of USP7 reveals post-translational modification sites and structural requirements for substrate processing and subcellular localization pptx

... 208-1102. In both digestion products, the cleavage site protected by the presence of ubiquitin was Ser341, located in the ‘fingers’ region of the catalytic core domain involved in the recognition of the ... oligomeriza- tion events. Deletion of the N- and C-terminal domains of USP7 affected the activity of the enzyme, with the C-terminus having a major i...
Ngày tải lên : 07/03/2014, 05:20
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Báo cáo khoa học: Biochemical characterization of annexin B1 from Cysticercus cellulosae pdf

Báo cáo khoa học: Biochemical characterization of annexin B1 from Cysticercus cellulosae pdf

... to the glycosaminoglycan. For annexin A5, a model of heparin binding has been put forward in which recognition and binding of heparin occurs on the concave side of the protein via the two binding ... the calcium-binding sites on the convex domain. Assuming the presence of further heparin-binding sites elsewhere on the protein surface, annexin B1 can remain bound to...
Ngày tải lên : 07/03/2014, 12:20
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Báo cáo khoa học: Functional characterization of front-end desaturases from trypanosomatids depicts the first polyunsaturated fatty acid biosynthetic pathway from a parasitic protozoan ppt

Báo cáo khoa học: Functional characterization of front-end desaturases from trypanosomatids depicts the first polyunsaturated fatty acid biosynthetic pathway from a parasitic protozoan ppt

... make them easy to recognize. They contain a fused cytochrome b5 as an N-terminal domain and three histidine boxes in the desaturase (C-terminal) domain. The third histidine box has the QX 2 HH instead ... of front-end desaturases are present in each of these trypanosomatid proteins, including the three highly conserved histidine boxes and the cytochrome b5 N-terminal domain...
Ngày tải lên : 07/03/2014, 12:20
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Báo cáo khoa học: Molecular characterization of a blood-induced serine carboxypeptidase from the ixodid tick Haemaphysalis longicornis docx

Báo cáo khoa học: Molecular characterization of a blood-induced serine carboxypeptidase from the ixodid tick Haemaphysalis longicornis docx

... as lmol of leucine, alanine or tyrosine released per min per mg of protein. The kinetic parameters k cat and K m were obtained from Hanes– Woolf plots. To examine whether rHlSCP1 has the ability ... Tokyo, Japan). The densities of stained bands were measured using image j 1.36b (National Insti- tutes of Health) and the ratio of the density divided by that of the contro...
Ngày tải lên : 16/03/2014, 10:20
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