... RanGAP1. Their comparison provides insight into the
protein interactions underlying sumoylation, and suggests how SUMO
proteins may be translocated between enzymes during the various steps of
the protein ... final
product of the sumoylation cascade [SUMO(C)].
The modeled and resolved structures discussed
above allow rationalization of the protein interactions
occur...
... complexed with the minor groove binding drug netrop-
sin. Biochemistry 36, 479 2–4 799.
13 Patel DJ (1982) Antibiotic–DNA interactions – inter-
molecular nuclear Overhauser effects in the netropsin-
d(CGCGAATTCGCG) ... cataloging
the structure to class I. As the bulky NH
2
group on guanine was believed
to prevent binding of the drug in the minor groove, the detailed...
... are in keeping with other LDHs.
With regard to the nature of the rate-determining
steps, conformational rearrangement is the predomin-
ant kinetic barrier in the single-turnover reaction, i.e.
in ... determine the pKa of the ionizable group at the active site. The
K
M
for pyruvate was determined from rates measured for 8–1 0 dif-
ferent pyruvate concentrations in 2...
... probably be
able to inhibit protein protein interactions in which
Keywords
activator protein- 1; coiled coils; electrostatic
interactions; protein design; protein folding
Correspondence
J. M. Mason, ... permitting a rigid interpretation in distinguish-
ing interacting from noninteracting proteins. One
Fig. 1. Schematics of library designs. The helical wheel diagram looks dow...
... cannot be predicted from the amino acid
sequence, we were very interested in analyzing whether
ILT2 nitration affected the Tyr residues in the hydro-
phobic interdomain that binds HLA-G. To address
this ... loss [23], protein nitration can also
elicit increased biological activity, such as in cathep-
sin D [24], or in the glucocorticoid receptor, where
nitration leads to a...
... contains three TPR motifs
forming the docking site for Hsp90-bound preproteins. AtToc12 (12)
exposes a J-domain (J) into the intermembrane space and has a
role in anchoring Hsp70, thereby assisting ... translocation of the photosyn-
thetic preproteins (down-regulated in the mutants) is
unclear. However, many proteins that are not involved
in photosynthesis (termed ‘housekeeping...
... Transmembrane domains are
depicted as columns. Regions involved in membrane binding are coloured in red, motifs involved in protein protein interaction are blue and
the dehydrogenase domains of Tic32 ... hypothesized that the protein exists in a
closed conformation, in which the TPR domain
shields the Hip/Hop-domain from the chaperone.
Surprisingly, the TPR motifs...
... for the interaction of the preprotein
with the receptor. The 16 ⁄ 23 chimera is retarded dur-
ing translocation; early in the process, the protein
assumes a structure within the membrane in which ... an N-terminal domain called the GTPase-
containing domain (G-domain). CpSRP54 also has a
second domain designated the methionine-rich
domain (M-domain) [48].
Within cpSRP4...
... mutants (the IEP of the
Ll.ltrB intron of Lactococcus lactis) have suggested
that the N-terminus of the RT domain is required for
protein interactions with the high-affinity binding site
in subdomain ... splicing of the intron RNA into
the insertion site in the DNA are shown at the top. Solid lines indicate DNA and dashed lines correspond to intron RNA.
M. D. Molina-Sa...
... (Fig. 1A,
lanes 4–6 ). The import of QORH into the inner enve-
lope of chloroplasts was shown to depend on targeting
information present in the C-terminus of the pre-
protein [11]. Therefore, we ... binding and hydrolysis [ 4–7 ].
Toc34
GDP
interacts with the second G -protein in the
Toc complex, Toc159, and simultaneously transfers the
preprotein to Toc159. Toc159 ac...