... ª 2009 The Authors Journal compilation ª 2009 FEBS
Channel-forming activities of peroxisomal membrane
proteins from the yeast Saccharomyces cerevisiae
Silke Grunau
1,2,
*, Sabrina Mindthoff
1,
*, ... peroxisomes from the yeast Saccharomyces cerevisiae grown
on oleic acid were investigated for the presence of channel (pore)-forming
proteins in the mem...
... Oxidative stress response in the
yeast Saccharomyces cerevisiae. Yeast 14, 1511–1527.
22 Moradas-Fereira P & Costa V (2000) Adaptive response
of the yeast Saccharomyces cerevisiae to reactive oxygen
species: ... that, at
least in part, the Ca
2+
flux comes from outside the
cells. Following the addition of BAPTA, the pattern of
the luminescence spectrum re...
... PCR’, using the first PCR product as
template. To make use of the BamHI site in the vector and
the XbaI site in the EhMGL1 gene, the product of the nes-
ted PCR was replaced with the corresponding ... isozymes, and that the degradation
of TFM is less sensitive to alterations of these residues than is the degrada-
tion of physiological substrates. These results sup...
... interfere with the pyrimid-
ine nucleotide pools of this pathogen.
All DHODH proteins of family 2 must be translo-
cated from the cytosol to the inner membrane of mito-
chondria. The proteins are ... showed that the purified enzymes were of the
expected molecular mass of 48 kDa for the full-length
enzyme and 42 kDa for the truncated enzyme. The
yield of re...
... regarding the importance of the pro-
nounced secondary structure in this region. Mutation of
either proline could result in reduction or increase in the
length of the helical secondary structure of the ... facing the inside of the cell. Five of the residues lie
within the previously characterized N-terminal regulatory
domain and two mutations are located within...
... as the number of
web pages containing the words the and ’and’.
891
matched. On the basis of these, we then calculate
the probability of a certain qualia element given a
certain role on the ... clue c, the query c(n) is sent to the web
search engine and the abstracts of the first m docu-
ments matching this query are downloaded. Then
the snippets are processed to find...
... 8.5, we studied thermal
stability of 17b-HSDcl from CD and UV melting
curves, from which the temperature of denaturation
(T
d
) and the van’t Hoff enthalpy of denaturation
(DH°
vH
)of1 7b-HSDcl were ... Inspection of Fig. 2B reveals two transitions,
the first in the pH range pH 7–5, and the second in
the pH range 5–3. The observed transitions in the aci-
dic pH ran...
... examine the topology of the binding site of the anti-MP8
Ig, 3A3, especially to evaluate the size of the cavity
remaining around the iron atom. For this purpose, we
studied the formation of Fe(II)-nitrosoalkane ... regioselectivity of the reaction in favor of the
formation of 2-nitrophenol [3]. Consequently, it was tempt-
ing to examine whether the hemoabzyme...
... their membranes, and thus
the lack of a specific molecular microbial target minimizes
resistance development. The fundamental principle of
action of most peptides depends on the ability of these
molecules ... concentrations of 10 l
M
to
1000 l
M
and at higher concentrations the peptide preci-
pitated. Thus, the index expresses the level of antibacterial
activity in re...
... arms of the replication fork, the
lagging and the leading strand, respectively [61,64]. We
therefore favour the idea that physical uncoupling of the
leading strand and the lagging strand DNA synthesis
may ... Analysis of the
essential functions of the C-terminal protein ⁄ protein
interaction domain of Saccharomyces cerevisiae pol e
and its unexpected ability to s...