Báo cáo khoa học: The interaction of the Escherichia coli protein SlyD with nickel ions illuminates the mechanism of regulation of its peptidyl-prolyl isomerase activity ppt
... of the Escherichia coli protein SlyD with
nickel ions illuminates the mechanism of regulation of its
peptidyl-prolyl isomerase activity
Luigi Martino
1,2
, Yangzi He
1,
*, Katherine L. D. Hands-Taylor
1
, ... PPIase site of SlyD. Although coher-
ent with the fact that SlyD appears to interact in the
proximity of the nickel ion high-affinit...
... after 2 h of IPTG
treatment, and these were the conditions used for fur-
ther purification of the protein.
The exoribonucleolytic activity of the crude extracts,
before and after induction with IPTG, ... stalling of the enzyme in the vicinity of the
two secondary structures of the mRNA.
Metal dependency of the exoribonuclease activity
of RNase II
The...
... interpretation of
the state of cells is often ambiguous [10,11]. Problems in
the interpretation of the state of cells that have lost
culturability are due, not only to the absence of consensus
on the definition ... elongation, protein synthesis and glucose meta-
bolism was analysed, as well as the effect of different
aeration conditions on the regulation of meta...
... or thermal degradation [37].
To test whether this occurs in the case of the START
proteins, we analyzed the migration behavior of these
proteins in SDS gels under various conditions.
First, the ... the Coomassie stains of the
purified proteins. The apparent molecular masses of the
His-tag START proteins in the SDS ⁄ PAGE system were
slightly greater than the calcula...
... Transfection of vector alone does not
modify the percentage of attached cells. Therefore, the
control of SHIP2 (or his mutants) transfected cells
could be the result of either the suggested conditions
with ... associated with the cell–matrix adhe-
sion sites in a network of protein- like integrins and
F-actin [35]. Our hypothesis is that the interaction of
SHIP2...
... the com-
mon deletion. By applying the specific probe, we can
even detect the presence of the common deletion in the
inner ear in the control group. The proportions of the
common deletion in the ... representative
results for relative abundance of the protein tested. As
compared with the control group, the expression of
TFAM protein in the inner ear of...
... solved the crystal structure of this GH5
enzyme in the presence of two different mechanism-
based inhibitors, thereby revealing the close network
of interactions that hold the terminal glucose of the ... help explain their importance to the specificity and
catalytic efficiency of the enzyme.
The role of the Phe-Phe entranceway
The fortuitous discovery of an u...
... to pH 2.8. Elution of the protein using an
eluant of low pH effectively removes the metal ions from
the protein, which are then separated from the protein band
as a result of the size-exclusion ... the coordination of
the fifth Cd
2+
ion by thiolate ligands within the clus-
ter, and, more specifically, within the crevice site of the
protein.
The biologica...
... all stages
of the reaction. The best explanation for these results may
be that the reaction of SDTG at the binding site of one
subunit changes the conformation of the o ther subunit,
thereby completely ... the
dynamics of this enzyme. A plot of the crystallographic B-
factors along the polypeptide chain can give an indication
of the relative flexibility of...
... the hSuv3p
domain that is necessary for interaction with HBXIP. (B) In vitro
interaction of hSuv3p with HBXIP. The SDS ⁄ PAGE analysis of the
binding of wildtype hSuv3p or its mutant devoid of ... in the truncated protein levels
within 4 h following inhibition of protein synthesis by
cycloheximide. We next wanted to examine whether the
presence of HBXIP bin...