Báo cáo khoa học: The interaction of the Escherichia coli protein SlyD with nickel ions illuminates the mechanism of regulation of its peptidyl-prolyl isomerase activity ppt

Báo cáo khoa học: The interaction of the Escherichia coli protein SlyD with nickel ions illuminates the mechanism of regulation of its peptidyl-prolyl isomerase activity ppt

Báo cáo khoa học: The interaction of the Escherichia coli protein SlyD with nickel ions illuminates the mechanism of regulation of its peptidyl-prolyl isomerase activity ppt

... of the Escherichia coli protein SlyD with nickel ions illuminates the mechanism of regulation of its peptidyl-prolyl isomerase activity Luigi Martino 1,2 , Yangzi He 1, *, Katherine L. D. Hands-Taylor 1 , ... PPIase site of SlyD. Although coher- ent with the fact that SlyD appears to interact in the proximity of the nickel ion high-affinit...
Ngày tải lên : 30/03/2014, 01:20
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Báo cáo khoa học: A single mutation in Escherichia coli ribonuclease II inactivates the enzyme without affecting RNA binding pot

Báo cáo khoa học: A single mutation in Escherichia coli ribonuclease II inactivates the enzyme without affecting RNA binding pot

... after 2 h of IPTG treatment, and these were the conditions used for fur- ther purification of the protein. The exoribonucleolytic activity of the crude extracts, before and after induction with IPTG, ... stalling of the enzyme in the vicinity of the two secondary structures of the mRNA. Metal dependency of the exoribonuclease activity of RNase II The...
Ngày tải lên : 30/03/2014, 15:20
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Báo cáo khoa học: Are UV-induced nonculturable Escherichia coli K-12 cells alive or dead? ppt

Báo cáo khoa học: Are UV-induced nonculturable Escherichia coli K-12 cells alive or dead? ppt

... interpretation of the state of cells is often ambiguous [10,11]. Problems in the interpretation of the state of cells that have lost culturability are due, not only to the absence of consensus on the definition ... elongation, protein synthesis and glucose meta- bolism was analysed, as well as the effect of different aeration conditions on the regulation of meta...
Ngày tải lên : 31/03/2014, 01:20
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Báo cáo khoa học: Cholesterol interaction with the related steroidogenic acute regulatory lipid-transfer (START) domains of StAR (STARD1) and MLN64 (STARD3) ppt

Báo cáo khoa học: Cholesterol interaction with the related steroidogenic acute regulatory lipid-transfer (START) domains of StAR (STARD1) and MLN64 (STARD3) ppt

... or thermal degradation [37]. To test whether this occurs in the case of the START proteins, we analyzed the migration behavior of these proteins in SDS gels under various conditions. First, the ... the Coomassie stains of the purified proteins. The apparent molecular masses of the His-tag START proteins in the SDS ⁄ PAGE system were slightly greater than the calcula...
Ngày tải lên : 30/03/2014, 04:20
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Báo cáo khoa học: SHIP2 interaction with the cytoskeletal protein Vinexin ppt

Báo cáo khoa học: SHIP2 interaction with the cytoskeletal protein Vinexin ppt

... Transfection of vector alone does not modify the percentage of attached cells. Therefore, the control of SHIP2 (or his mutants) transfected cells could be the result of either the suggested conditions with ... associated with the cell–matrix adhe- sion sites in a network of protein- like integrins and F-actin [35]. Our hypothesis is that the interaction of SHIP2...
Ngày tải lên : 07/03/2014, 12:20
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Báo cáo khoa học: Mitochondrial transcription factor A overexpression and base excision repair deficiency in the inner ear of rats with D-galactose-induced aging pdf

Báo cáo khoa học: Mitochondrial transcription factor A overexpression and base excision repair deficiency in the inner ear of rats with D-galactose-induced aging pdf

... the com- mon deletion. By applying the specific probe, we can even detect the presence of the common deletion in the inner ear in the control group. The proportions of the common deletion in the ... representative results for relative abundance of the protein tested. As compared with the control group, the expression of TFAM protein in the inner ear of...
Ngày tải lên : 14/03/2014, 23:20
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Báo cáo khoa học: Carbohydrate binding sites in Candida albicans exo-b-1,3-glucanase and the role of the Phe-Phe ‘clamp’ at the active site entrance ppt

Báo cáo khoa học: Carbohydrate binding sites in Candida albicans exo-b-1,3-glucanase and the role of the Phe-Phe ‘clamp’ at the active site entrance ppt

... solved the crystal structure of this GH5 enzyme in the presence of two different mechanism- based inhibitors, thereby revealing the close network of interactions that hold the terminal glucose of the ... help explain their importance to the specificity and catalytic efficiency of the enzyme. The role of the Phe-Phe entranceway The fortuitous discovery of an u...
Ngày tải lên : 15/03/2014, 23:20
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Báo cáo khoa học: Metal exchange in metallothioneins – a novel structurally significant Cd5 species in the alpha domain of human metallothionein 1a ppt

Báo cáo khoa học: Metal exchange in metallothioneins – a novel structurally significant Cd5 species in the alpha domain of human metallothionein 1a ppt

... to pH 2.8. Elution of the protein using an eluant of low pH effectively removes the metal ions from the protein, which are then separated from the protein band as a result of the size-exclusion ... the coordination of the fifth Cd 2+ ion by thiolate ligands within the clus- ter, and, more specifically, within the crevice site of the protein. The biologica...
Ngày tải lên : 16/03/2014, 06:20
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Báo cáo khóa học: S-(2,3-Dichlorotriazinyl)glutathione A new affinity label for probing the structure and function of glutathione transferases potx

Báo cáo khóa học: S-(2,3-Dichlorotriazinyl)glutathione A new affinity label for probing the structure and function of glutathione transferases potx

... all stages of the reaction. The best explanation for these results may be that the reaction of SDTG at the binding site of one subunit changes the conformation of the o ther subunit, thereby completely ... the dynamics of this enzyme. A plot of the crystallographic B- factors along the polypeptide chain can give an indication of the relative flexibility of...
Ngày tải lên : 16/03/2014, 16:20
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Báo cáo khoa học: Human ATP-dependent RNA ⁄ DNA helicase hSuv3p interacts with the cofactor of survivin HBXIP ppt

Báo cáo khoa học: Human ATP-dependent RNA ⁄ DNA helicase hSuv3p interacts with the cofactor of survivin HBXIP ppt

... the hSuv3p domain that is necessary for interaction with HBXIP. (B) In vitro interaction of hSuv3p with HBXIP. The SDS ⁄ PAGE analysis of the binding of wildtype hSuv3p or its mutant devoid of ... in the truncated protein levels within 4 h following inhibition of protein synthesis by cycloheximide. We next wanted to examine whether the presence of HBXIP bin...
Ngày tải lên : 23/03/2014, 15:21
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