... pantotrophus.
In uence of variations of conserved residues on
the in vivo activity of NirF
Interestingly, like NirN, NirF shares sequence similar-
ity with the C-terminal d
1
heme-containing domain of
cytochrome ... and of the
kanamycin insertion mutant nirF strain
(SBN03) in the upper right panel. Growth of
SBN11 (DnirF) is shown in the bottom left
panel and of SBN13...
... targets).
Effects of DNA cis-platination on sequence-
specific DNA binding of p73 proteins
We tested the in uence of DNA modification with
cisplatin on the binding of two p73 isoforms, p73d and
p73b, to the p21 ... weak
p53 binding (about 4% for mdm2 and PGM1 and
10% for p21).
Sensitivity of the sequence-specific p53 DNA
binding to DNA cis-platination depends on the
incidence o...
... represents the amino terminus of the pro-
tein. Using this method, we showed that asparagine 26
represents the amino terminus of P40. Knowing, that
the first amino acids of the processed P40 and P90 is
the ... state of this
peptide is unexpected: a peptide containing two basic
amino acids (arginine and histidine) and a blocked
amino terminus should not acquire more than two
posit...
... C-terminal cytosolic domain
of SR-BI which is a critical domain involved in selective
CE-uptake [29,53] is lacking in SR-BII and the lack of
SR-BII protein in all three choriocarcinoma cell lines
investigated ... choriocarcinoma
cells (24 h in DMEM containing 10% FBS, 1% glutamine
and 1% penicillin/streptomycin) and CHO cells (HAM’s
F12 medium containing 5% (v/v) FBS, 2 m
M...
... data
AcLDL
1
2
Visfatin/cyclophilin mRNAVisfatin/cyclophilin mRNA
Visfatin/cyclophilin mRNA
CD36/cyclophilin mRNA
FABP4/cyclophilin mRNA
Visfatin/cyclophilin mRNA
Visfatin/cyclophilin mRNA
Visfatin/cyclophilin mRNA
3
4
3 ... adipocytes, and mostly
by macrophages, and circulates in the plasma of
humans and mice [4]. Plasma concentrations of visfa-
tin are positively associated with...
... confirm
incorporation of ubiquitously labeled arginine into cyl-
indrospermopsin [23]. However, the transamidination
of glycine from arginine by amidinotransferase, yield-
ing guanidinoacetate, is common in vertebrates, ... performances.
However, the kinetic mechanism of CyrA in the pres-
ence of l-arginine and glycine as substrates differs
from the well-established ping-pong mec...
... predicting the role of Ca
2+
and
Ca
2+
-binding proteins in biological systems.
Materials and methods
Molecular cloning and modelling of engineered
CD2.STIM1.EF
The single EF-hand motif in STIM1 ... distance between
the two termini of the inserted Ca
2+
-binding sites in
the model structure of the EF-hand of STIM1 is within
15 A
˚
. Accordingly, a total of six glycin...
... carcinoma cells [13]. Further studies have
shown that phosphorylation of Bcl-2 on residues of in
its loop domain, including Ser70 and Ser87, is critically
involved in the apoptotic process, and ... were introduced into
the cells using Lipofectamine 2000 (Invitrogen) according
to the manufacturer’s recommendations.
Quantification of cell death
Apoptosis was investigated by Annex...
... Calp1 binding site is
included in the C-terminal domain of the ask molecule,
and this binding is independent of the susceptibility of the
a-actinin isoform to calpain proteolysis.
Identification of ... (domains III–IV) of the 80 kDa subunit,
are implicated in the interface linking calpain 1 to skeletal
muscle a-actinin.
Colocalization of microcalpain and a-actinin
in myog...
... in TPb mRNA expression in
response to 15d-PGJ
2
in HEL cells was reflected in a
corresponding reduction in the overall level of TP
expression, as assessed by radioligand binding studies,
and in ... vasculature and point to further critical differences
into the modes of regulation of TPa and TPb in
humans. Moreover, in view of the critical link between
the enhance...