... estimate obtained from grafit software.
Kinetics of reversible inhibition of AspB, T141A
and H188A by 3-nitropropionate and
D-malate
The reversible inhibition of AspB and the T141A and
H188A mutants ... from Bacillus sp. YM55-1
FEBS Journal 276 (2009) 2994–3007 ª 2009 The Authors Journal compilation ª 2009 FEBS 3007
Site-directed mutagenesis, kinetic and in...
... increasing concentrations of XAIP (A) and of a-amylase from
Bacillus licheniformis with increasing concentrations of XAIP (B).
S. Kumar et al. Crystal structure and inhibition studies of XAIP
FEBS Journal ... internal space of the TIM barrel
considerably. Of the three observed cis peptides, two
(Gly33–Phe34 and Trp253–Asp254) are found at the
ends of b-strands b2...
... the
structures of PSs from E. coli and M. tuberculosis.
Structural analyses
Analysis of the sites of substrate binding from NMR data,
and structural comparison of the E. coli, M. tuberculosis
and A. ... Enzymatic
and other biochemical studies have shown that for PS
from M. tuberculosis, pantoate, ATP and b-alanine
have K
m
values of 0.13 mm, 2.6 mm and 0.8 mm,
re...
... 38-kDa spe-
cies and Ser-Asp-Met-Glu-Lys- for the 19-kDa species,
strongly suggesting that respective protein bands
Fig. 1. Schematic representation of the structure of the precursor
of kumamolisin-As, ... 7.0 and 4 °C for 6 h, followed by SDS ⁄ PAGE. The rate of ligation was estimated from percentage
intensity of the band of the 57-kDa protein in a total of intensities o...
... layer of vinyl + 3 layers of curing blanket +
roof
20 days
(a) Temperature analysis of the total cross section of mat foundation
(b) Stress dispersion of the total cross section of ... during and after curing
(c) TCI of the total cross section of mat foundation during and after curing
Figure 5. Full-Scale thermal stress and TCI of mat foundation
4....
... matching parts of the sense and
antisense strands of the DNA sequence were used. The
synthesized fragment was inserted between the BamHI
and XhoI sites of pGEX-6P-1. Nucleotide sequences of
the recombinant ... replicate
experiments.
21
0
119
90
65
4
9
3
7
2
9
(
kDa
)
21
9
S
I
S
ISISI
S
I
S
I
4
AaCter
TpN15
TpN17
T
pN47
4
A
aCt
e
r
-
TpN15
ATG
4
A
aCte
r
TpN15
T
pN17
TpN47
4
Aa
C
t...
... this question. Seven PLAs and five 3FTx were purified from the
KBf venoms, and respective cDNAs were cloned from venom glands of
one of the snakes. Comparison of their mass and N-terminal sequence
revealed ... 525
Sequences, geographic variations and molecular
phylogeny of venom phospholipases and threefinger toxins
of eastern India Bungarus fasciatus and kinetic anal...
... 85% inhibition of
BHLmL2 and BHAb, respectively, while 1 mm 2-mer-
captoethanol caused 85% and 97% inhibition of
BHLmL2 and BHAb, respectively.
The influence of pH on the activities of the BH
enzymes ... modification
of Asp, Glu, Cys and Ser are shown in Table 5. No
significant inactivation of BHs from either L. maculans
or A. brassicicola was observed upon treatment wi...
... on splicing.
A variety of tools are available to us for this purpose
and further investigation of these sequence variants will
not only further our understanding of the splicing
mechanism and ... and further elucidation of this complex
and interesting system. Evolutionary studies represent
another important field for the investigation of the ele-
ments involved in splicing...
... those from a mouse antibody to
avoid immunogenicity of the antibody framework from
a different species [23–25]. The success of the series of
studies shows that the stable framework structure of
Fv ... 38]
Y/S library
in all the CDRs of heavy chain
and CDR3 of light chain
[ref 39]
10
FN3
Randomization in CDR loops
of heavy and light chains
Randomization
in BC, DE and...