... al. Mitochondrial transport of thiamine pyrophosphate
FEBS Journal 277 (2010) 1172–1181 ª 2010 The Authors Journal compilation ª 2010 FEBS 1181
The biochemical properties of the mitochondrial thiamine
pyrophosphate ... congested-like tracheae
gene of Drosophila melanogaster encodes a member of
the mitochondrial carrier family required for gas-filling
of...
... used for the
quantification of the enzymatically formed H
2
S.
Measurement of pyruvate formation
The activity of the purified d-CDes enzyme was followed
through the measurement of the amount of pyruvate
formed ... through an inhibition of threonine deaminase.
On the other hand, the presence of the yedO gene
stimulates cell growth in the presence of d-cysteine...
... increased. The results suggested
that the effect of salts on the oligomeric state of
CDase I-5 correlated with the dissociation of the
dodecameric form of the enzyme.
Discussion
An earlier study on the ... 7.0. The tertiary structure indicated that
the effect of pH on dissociation of the oligomer was mainly due to the pro-
tonation of H539. Unlike the pH-...
... several
repetitions of the motif. Repetition of the motif seems to
determine the specificity of binding to the target RNA
sequence as well. Indeed, analyzing the PPR proteins of the
Arabidopsis genome ... deletion of
seven out of nine did not change the RNA-binding
properties [41]. Defining the exact structure of the
HCF152 homodimer together with the petB pr...
... the projection of the tilt of the z axis on the x¢,y¢ plane (defined direction of tilt of z and the x¢ axis), and j % a + c defines the
location of the rhombic (x, y) axes. The orientation of the ... at position E7 as
the source of the H-bond to ligand on the basis of a
partial sequence, which had indicated a Tyr on the distal
E-helix. The similarity...
... positions of molecular mass markers are shown to the left,
and the size in base pairs of the products to the right (the Meis2 equivalent of Meis3.2 would be expected at 149 bp). (C, D) RNA from a
series ... However, the activity of the full-length Meis1a
was not tested, and on the basis of our work we expect
that its activity would be inhibited by the conserved
Ht...
... of a severe mutant. At the other
end of the spectrum, where we have low energy, there
is 75% probability for the mutation to be nonsevere if
the energy is 0.125 or lower. Thus, on the basis of ... algorithms have been developed recently [4–7].
The major drawback of these analyses is the lack of
information regarding the activity or loss of activity
of the targe...
... obtained with the transition at posi-
tions 1915 and 1917. These mutations had an effect on
the formation of pseudouridine exclusively at the
mutant position and did not affect either of the other
two ... by
Southern analysis. The rluD::cat strain had a full deletion
of both rluD and yfiH, together with their annotated pro-
moter sequences. The inserted cat cassette had a dis...
... Given that the roles
of these residues definitely confirm each other, the dif-
ference in their significance might be attributable to
the importance and ⁄ or necessity of the receipt of the
phenol-hydroxyl ... environments of BPA in the ligand-binding pocket
of the ERRc. The proximity of each amino acid residue (within a
distance of 5 A
˚
) to BPA is shown in the...
... each extremity of the polypeptide
chain, must of necessity arise from contacts that are
made outwith the active site of the enzyme.
The effect of removal of the N-terminal extension
The full N-terminal ... residues
69–81 from the S. castellii sequence is small compared
to that achieved by location of the full-length extension
at the N-terminal end of the...