... reactions
essential for growth and proliferation, is uncertain. Hence, it
is of interest to investigate the uptake and metabolism of
NTBI in normal and cancer cells, and the effect of Fe
chelators on these ... overload
conditions, and is also implicated in intracellular Fe
metabolism [3,9,10]. An important aspect of this work
was the assessment of the effect of novel Fe...
... to
indicate the presence of the specific holocytochrome
under investigation if the features of the spectrum, in
terms of wavelength maxima, and the position and
intensity of heme-staining bands on SDS-PAGE ... heme (saturation of
each heme vinyl group on formation of a c-type cyto-
chrome causes a blue shift of 2–3 nm in the a-band of
the absorption spectrum). To confirm t...
... inhibited the proliferation of MCF-7 cells and induced cell death. (A) MCF-7 cells were treated with different concentrations
of curcumin for one cell cycle and the inhibition of cell proliferation was ... 1C,D). For example, 4 and 24% of MCF-7 cells
showed nuclear localization of p53 (Fig. 1C), whereas
7 and 33% of cells showed nuclear localization of p21
in the...
... using a
PerkinElmer L S-50 fluorimeter at room temperature with a
scan speed of 500 nmÆmin
)1
and a n excitation wavelength of
280 nm, and scanned over the range 30 0–4 50 nm. An
accumulation of eight ... this
method contained a mixture of proteins B and B¢.The
relative abundance of p roteins B and B¢ was assessed b y
using SDS/PAGE and electrospray ionization (ESI)-MS.
I...
... in the
absence of sulfide, but artificial incorporation of Fe
3+
was not detected (not shown).
The presence of iron–sulfur clusters was investigated
by quantification of nonheme iron and acid-labile ... Lake,
Michigan, USA) containing an atmosphere of 97% N
2
and
3% H
2
. Cells were lysed with B-Per (Pierce, Rockford, IL,
USA), and detergent and cell debris were removed by ce...
... Differ
16, 36 8–3 77.
6 Subramaniam S & Unsicker K (2009) ERK and cell
death: ERK1 ⁄ 2 in neuronal death. FEBS J.
7 Teixeiro E & Daniels MA (2009) ERK and cell death:
ERK location and T cell selection. ... 59, 7 6–8 6.
ERK and cell death S. Cagnol and J C. Chambard
20 FEBS Journal 277 (2010) 2–2 1 ª 2009 The Authors Journal compilation ª 2009...
... 2-implicated cell death paradigms.
ERK1
⁄
2 in cellular models of neuronal
death
The first evidence for a role of ERK1 ⁄ 2 in cell death
was demonstrated in the oligodendroglial cell line,
CG4. ... plasma
membrane damage and independently of caspase-3.
J Cell Biol 165, 35 7–3 69.
35 Mebratu Y & Tesfaigzi Y (2009) How ERK1 ⁄ 2 acti-
vation controls cell prolife...
... MINIREVIEW
ERK and cell death: ERK location and T cell selection
Emma Teixeiro and Mark A. Daniels
Department of Molecular Microbiology and Immunology, Center for Cellular and Molecular Immunology, ... activity and subcellular
location are the same regardless of the ligand strength,
whereas ERK activity and location change depending
of the nature of the sele...
... the result of cell death
and not due to an inhibition of cell proliferation, as
determined by cell counting as well as quantification of
loss of cell integrity [22]. Although the ERK prodeath
effect ... protection
against cell death by apoptosis, it is now clear that ERK can also be
directly linked to cell death signaling. Here, we review the role of ERK i...