... from C. coturnix F. Feng et al.
1584 FEBS Journal 278 (2011) 1573–1584 ª 2011 The Authors Journal compilation ª 2011 FEBS
Gene cloning, expression and characterization of avian
cathelicidin orthologs, ... aberrant expression of cathelici-
dins is often associated with various disease processes
[26,27].
In the present study, the gene cloning and character-
ization...
... 4787
Molecular cloning, expression and characterization of
protein disulfide isomerase from Conus marmoreus
Zhi-Qiang Wang
1
, Yu-Hong Han
1,2
, Xiao-Xia Shao
1
, Cheng-Wu Chi
1,2
and Zhan-Yun Guo
1
1 ... rate of decrease of the linear form, the original data were fitted
by Y(t) ¼ e
–kt
· 100%, where Y is the percentage of the linear form, and t is the refolding time. For th...
... HRT1 and HRT2 in E. coli and purification
of HRT2. The pETHRT1 and pETHRT2 were constructed and
introduced into E. coli BL21(DE3). The expression of these genes were
induced by addition of 0.5 ... shown in Fig. 3
(A1 and A2) both probes gave specific hybridization bands
of 1 kb which are consistent with the sizes of each of the
cDNAs. The expression patterns of HRT1...
... primordial
gene resulting in the STAT5 and STAT3 genes as two
different lineages [40]. The positions of introns in the
TfSTAT5 gene (Table 1, and Fig. 2) were almost identical
to those of MmSTAT5a and ... STAT1 gene [38].
Recently, the genomic structures of mouse STAT3,
STAT5a, STAT5b and zebrafish STAT3 have also been
identified and characterized [40]. The MmSTAT5b and...
... nto
the same expression vector under the control of the T7
promoter, and coexpression of oraE and oraS genes was
carried out in E. co li. Meanwhile, the extent of the
involvement of AdoCbl or ... con-
taining both genes was cloned into the same expression
vector an d coexpression of the oraE and oraS genes was
carried out in E. coli . The solubility of the coexpressed
Or...
... described for PRAI and
FLAG-PRAI. The final yield of soluble, purified trPRAI-
His was 20–30 mg per litre of induced culture.
Characterization of trPRAI-His
The far-UV and near-UV CD spectra of trPRAI-His
were ... selection and characterization of a stable
subdomain of phosphoribosylanthranilate isomerase
Wayne M. Patrick
1,2
and Jonathan M. Blackburn
1,3,4
1 Department...
... structures of EcR and
USP from L. decemlineata (b) the analysis of mRNA
expression profile of L. decemlineata, EcR and USP,
and (c) the measurement of the binding affinity of
steroidal and nonsteroidal ... 4119
and L. migratoria USP showed higher homology to
RXR of human and mouse than USP isoforms of dip-
teran and lepidopteran insects. Thus, the functions of
US...
... temporal expression of the B-FABP gene
reported here (Fig. 4C) and our previous report of its
expression in the periventricular grey zone of the optic
tectum of adult zebrafish brain, a site of neurogenesis ... a
FABP/ligand interaction [14]. The essential roles of DHA in
CNS development [1,2], the spatial and temporal expression
pattern of the B-FABP gene [11–13],...
... the expression of b-actin, at days 3, 7 and 14, (C) osteocal-
cin expression normalized for the expression of GAPDH and HPRT
at day 3, and (D) osteopontin expression, normalized for the
expression ... binding of the assay versus the log of concentra-
tions of standards. ‘Best fit’ curves were obtained with
graphpad prism software. On the basis of the standard
curve,...
... The
following expression burst of TetA and TetR leads to a
rapid reduction of the cytoplasmic tc concentration [10]
which, in turn, shuts expression of both genes off again.
Expression of TetA is ... requirements and open up new
applications for conditional regulation.
Expression can be switched between two alleles
of one gene
The expression of two genes or of tw...