Báo cáo Y học: Identification of a critical lysine residue at the active site in glyceraldehyde-3-phosphate dehydrogenase of Ehrlich ascites carcinoma cell ppt

Báo cáo Y học: Identification of a critical lysine residue at the active site in glyceraldehyde-3-phosphate dehydrogenase of Ehrlich ascites carcinoma cell ppt

Báo cáo Y học: Identification of a critical lysine residue at the active site in glyceraldehyde-3-phosphate dehydrogenase of Ehrlich ascites carcinoma cell ppt

... Identification of a critical lysine residue at the active site in glyceraldehyde-3-phosphate dehydrogenase of Ehrlich ascites carcinoma cell Comparison with the rabbit muscle enzyme Swapna Ghosh 1 , ... other hand, confirm that an essential lysine residue is involved in the catalytic activity of the EAC cell enzyme. This difference in the...
Ngày tải lên : 24/03/2014, 04:21
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Tài liệu Báo cáo Y học: Identification and characterization of a mammalian 14-kDa phosphohistidine phosphatase pdf

Tài liệu Báo cáo Y học: Identification and characterization of a mammalian 14-kDa phosphohistidine phosphatase pdf

... supernatant was used for purification of the recombinant protein. Purification of the recombinant phosphohistidine phosphatase A 9-mL sample of the supernatant of the bacterial lysate was chromatographed ... Protein kinases and phosphatases that act on histidine, lysine, or arginine residues in eukaryotic proteins: a possible regulator of the mitogen-activated protein...
Ngày tải lên : 21/02/2014, 01:21
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Tài liệu Báo cáo Y học: Identification of a set of genes involved in the biosynthesis of the aminonucleoside moiety of antibiotic A201A from Streptomyces capreolus pdf

Tài liệu Báo cáo Y học: Identification of a set of genes involved in the biosynthesis of the aminonucleoside moiety of antibiotic A201A from Streptomyces capreolus pdf

... suggest that certain enzymes, and therefore the corresponding genes of the A2 0 1A biosyn- thetic pathway, may be related to their counterparts of the puromycin and hygromycin A biosynthetic pathways, respectively. The ... Further identification and quantification of puromycin were achieved by a Pac enzymatic assay [21]. Preparation of 3¢-amino-3¢-deoxyadenosine 3¢-amino...
Ngày tải lên : 21/02/2014, 01:21
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Tài liệu Báo cáo Y học: Identification and characterization of the Escherichia coli stress protein UP12, a putative in vivo substrate of GroEL pptx

Tài liệu Báo cáo Y học: Identification and characterization of the Escherichia coli stress protein UP12, a putative in vivo substrate of GroEL pptx

... shown that GatY aggregates at 42 °Cinmutant cells containing a deletion for DnaK [35]. Taken together, these observations indicate that maintenance of the correct folding state of GatY in E. coli ... its steady-state level increases further during the late stationary phase. As a result of this accumulation, the relative amount of UP12 in stationary cells is about 10 ti...
Ngày tải lên : 22/02/2014, 07:20
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Báo cáo Y học: Identification and characterization of a new gene from Variovorax paradoxus Iso1 encoding N -acyl-D-amino acid amidohydrolase responsible for D-amino acid production pdf

Báo cáo Y học: Identification and characterization of a new gene from Variovorax paradoxus Iso1 encoding N -acyl-D-amino acid amidohydrolase responsible for D-amino acid production pdf

... specificity of the N-D-AAase protein, the activity of the enzyme against N-acyl- D -or L -amino acids and other D -amino acid derivates was determined (Table 3). The substrates analysed were a range of ... pH 6 and pH 8, 80% activity remained. The N-D-AAase had higher hydrolysing activity against N-ace- tyl- D -amino acid derivates containing D -methionine, D -leucine and D -...
Ngày tải lên : 08/03/2014, 16:20
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Báo cáo Y học: Identification of residues critical for activity of the wound-induced leucine aminopeptidase (LAP-A) of tomato pptx

Báo cáo Y học: Identification of residues critical for activity of the wound-induced leucine aminopeptidase (LAP-A) of tomato pptx

... C a of Asp a nd its carboxylate oxygen, C a of Arg and its side-chain amines or guanidinium, and C a of Lys and its side-chain amine was determined by measuring distances in the blLAP. These average ... bovine and prokaryotic LAPs and a limited mutational analysis of the E. coli PepA have suggested Lys262 and Arg336 have a role in catalysis [22,23]. The residues...
Ngày tải lên : 08/03/2014, 23:20
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Báo cáo Y học: Identification and characterization of a novel activated RhoB binding protein containing a PDZ domain whose expression is specifically modulated in thyroid cells by cAMP pot

Báo cáo Y học: Identification and characterization of a novel activated RhoB binding protein containing a PDZ domain whose expression is specifically modulated in thyroid cells by cAMP pot

... was secondary to the activation of the adenylyl cyclase/cAMP pathway, the same experiments were performed using 10 )5 M forskolin, an adenylate cyclase activator. A pattern similar to the thyrotropin ... cytoplasmic staining with a clear perinuclear accumulation (Fig. 4B). Moreover p76 RBE is, at least partially, located in the cell plasma membrane as indicated by arrows...
Ngày tải lên : 23/03/2014, 21:20
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Tài liệu Báo cáo Y học: Identification of syntaxin-1A sites of phosphorylation by casein kinase I and casein kinase II ppt

Tài liệu Báo cáo Y học: Identification of syntaxin-1A sites of phosphorylation by casein kinase I and casein kinase II ppt

... DISCUSSION Phosphorylation a nd dephosphorylation of protein su b- strates by k inases and phosphatases are enzymatic activities that play prominent roles in many biological processes including synaptic vesicle ... residue( s) a re located within t he N-terminal moiety of syntaxin- 1A (Fig. 2). Our data support previous reports indicating that CKII phos- phorylates the N-termina...
Ngày tải lên : 22/02/2014, 04:20
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Báo cáo Y học: Identification of the 19S regulatory particle subunits from the rice 26S proteasome potx

Báo cáo Y học: Identification of the 19S regulatory particle subunits from the rice 26S proteasome potx

... Biological Research/Graduate School of Integrated Science, Japan, personal communication) iden- tified N-acetylation of many of the yeast 26S proteasome subunits (a1 , a2 , a3 , a4 , a5 , a6 , a7 , a3 , a5 , ... 9S regulatory particles (RP; 700 kDa). The 20S proteasome has a cylindrical structure, consisting of a and b rings stacked in the order of abba, and each...
Ngày tải lên : 08/03/2014, 16:20
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Báo cáo Y học: Identification of residues in the PXR ligand binding domain critical for species specific and constitutive activation docx

Báo cáo Y học: Identification of residues in the PXR ligand binding domain critical for species specific and constitutive activation docx

... amino-acid analyzer combines the OPA and FMOC as derivatization reagents for the complete detection of all residues. BSA was used as a standard protein to calculate the amount of protein in the ... create a more pronounced change than the spatially and electrostatically moderate change of a histidine to a glutamine and thereby give additional information into its pot...
Ngày tải lên : 08/03/2014, 16:20
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