Báo cáo Y học: Identification of a critical lysine residue at the active site in glyceraldehyde-3-phosphate dehydrogenase of Ehrlich ascites carcinoma cell ppt
... Identification of a critical lysine residue at the active site in
glyceraldehyde-3-phosphate dehydrogenase of Ehrlich ascites
carcinoma cell
Comparison with the rabbit muscle enzyme
Swapna Ghosh
1
, ... other hand, confirm that an essential lysine residue is
involved in the catalytic activity of the EAC cell enzyme.
This difference in the...
... supernatant was used for purification of
the recombinant protein.
Purification of the recombinant phosphohistidine
phosphatase
A 9-mL sample of the supernatant of the bacterial lysate
was chromatographed ... Protein kinases and phosphatases that act
on histidine, lysine, or arginine residues in eukaryotic proteins: a
possible regulator of the mitogen-activated protein...
... suggest that certain enzymes, and
therefore the corresponding genes of the A2 0 1A biosyn-
thetic pathway, may be related to their counterparts of the
puromycin and hygromycin A biosynthetic pathways,
respectively.
The ... Further identification and quantification
of puromycin were achieved by a Pac enzymatic assay
[21].
Preparation of 3¢-amino-3¢-deoxyadenosine
3¢-amino...
... shown that GatY aggregates at 42 °Cinmutant
cells containing a deletion for DnaK [35]. Taken together,
these observations indicate that maintenance of the correct
folding state of GatY in E. coli ... its
steady-state level increases further during the late stationary
phase. As a result of this accumulation, the relative amount
of UP12 in stationary cells is about 10 ti...
... specificity of the N-D-AAase protein,
the activity of the enzyme against N-acyl-
D
-or
L
-amino acids
and other
D
-amino acid derivates was determined (Table 3).
The substrates analysed were a range of ... pH 6 and pH 8, 80% activity remained. The
N-D-AAase had higher hydrolysing activity against N-ace-
tyl-
D
-amino acid derivates containing
D
-methionine,
D
-leucine and
D
-...
... C
a
of Asp a nd its carboxylate oxygen,
C
a
of Arg and its side-chain amines or guanidinium, and C
a
of Lys and its side-chain amine was determined by
measuring distances in the blLAP. These average ... bovine and prokaryotic LAPs and a limited mutational
analysis of the E. coli PepA have suggested Lys262 and
Arg336 have a role in catalysis [22,23].
The residues...
... was secondary to
the activation of the adenylyl cyclase/cAMP pathway, the
same experiments were performed using 10
)5
M
forskolin,
an adenylate cyclase activator. A pattern similar to the
thyrotropin ... cytoplasmic
staining with a clear perinuclear accumulation (Fig. 4B).
Moreover p76
RBE
is, at least partially, located in the cell
plasma membrane as indicated by arrows...
... DISCUSSION
Phosphorylation a nd dephosphorylation of protein su b-
strates by k inases and phosphatases are enzymatic activities
that play prominent roles in many biological processes
including synaptic vesicle ... residue( s) a re located
within t he N-terminal moiety of syntaxin- 1A (Fig. 2). Our
data support previous reports indicating that CKII phos-
phorylates the N-termina...
... Biological Research/Graduate School of
Integrated Science, Japan, personal communication) iden-
tified N-acetylation of many of the yeast 26S proteasome
subunits (a1 , a2 , a3 , a4 , a5 , a6 , a7 , a3 , a5 , ... 9S
regulatory particles (RP; 700 kDa). The 20S proteasome
has a cylindrical structure, consisting of a and b rings
stacked in the order of abba, and each...
... amino-acid analyzer combines the OPA and
FMOC as derivatization reagents for the complete detection
of all residues. BSA was used as a standard protein to
calculate the amount of protein in the ... create a more pronounced change than the
spatially and electrostatically moderate change of a histidine
to a glutamine and thereby give additional information into
its pot...