Báo cáo khoa học: Functional site of endogenous phospholipase A2 inhibitor from python serum Phospholipase A2 binding and anti-in¯ammatory activity ppt

Báo cáo khoa học: Functional site of endogenous phospholipase A2 inhibitor from python serum Phospholipase A2 binding and anti-in¯ammatory activity ppt

Báo cáo khoa học: Functional site of endogenous phospholipase A2 inhibitor from python serum Phospholipase A2 binding and anti-in¯ammatory activity ppt

... Functional site of endogenous phospholipase A 2 inhibitor from python serum Phospholipase A 2 binding and anti-in¯ammatory activity Maung-Maung Thwin 1 , Ramapatna ... IC 50 , concentration of the inhibitor that inhibits PLA 2 activity by 50%; PIP, phospholipase inhibitor from python; PLI, phospholipase A 2 inhibitor; sPLA 2 , secretory phospholipa...
Ngày tải lên : 23/03/2014, 21:21
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Tài liệu Báo cáo khoa học: Functional characterization of an orphan cupin protein from Burkholderia xenovorans reveals a mononuclear nonheme Fe2+-dependent oxygenase that cleaves b-diketones ppt

Tài liệu Báo cáo khoa học: Functional characterization of an orphan cupin protein from Burkholderia xenovorans reveals a mononuclear nonheme Fe2+-dependent oxygenase that cleaves b-diketones ppt

... ligand binding, and the role of storage proteins, the cupins constitute one of the most diverse superfamilies of proteins known. They have been described from all three domains of life [1,2], and usually ... constitute a large and widespread superfamily of b-barrel proteins in which a mononuclear metal site is both a conserved feature of the struc- ture and a source...
Ngày tải lên : 18/02/2014, 06:20
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Tài liệu Báo cáo khoa học: Functional dissection of the Schizosaccharomyces pombe Holliday junction resolvase Ydc2: in vivo role in mitochondrial DNA maintenance pptx

Tài liệu Báo cáo khoa học: Functional dissection of the Schizosaccharomyces pombe Holliday junction resolvase Ydc2: in vivo role in mitochondrial DNA maintenance pptx

... between helices 3 and 4 at the junction crossover – and the unpairing of four base-pairs in the centre of the junctions [19]. Detailed understanding of the functional role of these interactions would ... well-defined dimer. The cata- lytic site consisted of two aspartates ) Asp46 and Asp230 ) which mapped to the catalytic site of RuvC. Protruding from opposite sides...
Ngày tải lên : 20/02/2014, 11:20
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Báo cáo khoa học: Functional characterization of artemin, a ferritin homolog synthesized in Artemia embryos during encystment and diapause doc

Báo cáo khoa học: Functional characterization of artemin, a ferritin homolog synthesized in Artemia embryos during encystment and diapause doc

... vitro,an activity characteristic of molecular chaperones and shown here to be shared by apoferritin and ferritin. This is the first report that apoferritin ⁄ ferritin may protect cells from stress ... denaturation of citrate synthase in vitro,a capability shared by apoferritin and ferritin, and con- fers stress tolerance on transfected mammalian cells. Artemin and ferritin ma...
Ngày tải lên : 16/03/2014, 11:20
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Báo cáo khoa học: Functional analysis of the methylmalonyl-CoA epimerase from Caenorhabditis elegans docx

Báo cáo khoa học: Functional analysis of the methylmalonyl-CoA epimerase from Caenorhabditis elegans docx

... be built, suggesting conservation of ligand and metal binding sites. Comparison with corresponding sequences from a variety of organisms shows more than 1 ⁄ 6 of the sequence is completely conserved. Mutants ... sequence from P. Sher- manii (MCEE_PROFR) are also labeled. Fig. 6. Model of MCE-1 structure. View along the length of the eight-stranded beta-barrel onto the putati...
Ngày tải lên : 23/03/2014, 13:20
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Báo cáo khoa học: Definition of the residues required for the interaction between glycine-extended gastrin and transferrin in vitro pptx

Báo cáo khoa học: Definition of the residues required for the interaction between glycine-extended gastrin and transferrin in vitro pptx

... Baker EN (2003) Structural and functional consequences of binding site mutations in transferrin: crystal structures of the Asp63Glu and Arg124Ala mutants of the N-lobe of human transferrin. Biochemistry ... absence or presence of increasing concentrations of unlabeled Ggly. The ApoTf–Ggly complex was separated from the unreacted Ggly by SDS ⁄ PAGE, and the extent of...
Ngày tải lên : 07/03/2014, 02:20
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Tài liệu Báo cáo khoa học: Functional studies of active-site mutants from Drosophila melanogaster deoxyribonucleoside kinase Investigations of the putative catalytic glutamate–arginine pair and of residues responsible for substrate specificity docx

Tài liệu Báo cáo khoa học: Functional studies of active-site mutants from Drosophila melanogaster deoxyribonucleoside kinase Investigations of the putative catalytic glutamate–arginine pair and of residues responsible for substrate specificity docx

... between the base and substrate -binding amino acid affected the binding of dThd and deoxycytidine (dCyd). Results We performed site- directed mutagenesis of four active site residues of Dm-dNK. Residue ... Structural alignment of wild-type Dm-dNK (blue) (PDB ID: 1OE0) and Dm -dNK-E52D (red) illustrating the binding of the feed- back inhibitor dTTP and the position...
Ngày tải lên : 19/02/2014, 02:20
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Báo cáo khoa học: Functional analysis of the aglycone-binding site of the maize b-glucosidase Zm-p60.1 pot

Báo cáo khoa học: Functional analysis of the aglycone-binding site of the maize b-glucosidase Zm-p60.1 pot

... levels of amino acid sequence similarity ranging from 45% to 53%, the architecture of the aglycone -binding site of Zm-p60.1 differs distinctly from that of Bgl4:1 and its homologs. These findings offer ... analysis of structural and catalytic prop- erties of the mutants, and simulations of the sub- strate–enzyme interactions of the wild-type and one of the mut...
Ngày tải lên : 16/03/2014, 04:20
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Báo cáo khoa học: Functional role of fumarate site Glu59 involved in allosteric regulation and subunit–subunit interaction of human mitochondrial NAD(P)+-dependent malic enzyme pptx

Báo cáo khoa học: Functional role of fumarate site Glu59 involved in allosteric regulation and subunit–subunit interaction of human mitochondrial NAD(P)+-dependent malic enzyme pptx

... quantitation of microgram quantities of protein, utilizing the principle of protein-dye binding. Anal Biochem 72, 248–254. 38 Segel IH (1993) Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and ... may act as an allosteric inhibitor of m-NAD-ME [18,21,25], and the allosteric properties of this isoform may relate to its particular role in the pathways of mala...
Ngày tải lên : 23/03/2014, 06:20
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Tài liệu Báo cáo khoa học: Functional analysis of the basic helix-loop-helix transcription factor DEC1 in circadian regulation ppt

Tài liệu Báo cáo khoa học: Functional analysis of the basic helix-loop-helix transcription factor DEC1 in circadian regulation ppt

... other h and, deletion of up to 273 residues from the C-terminal region of DEC1 (DEC1:1–309, DEC1:1–232 and DEC1:1–139) had little effect on the suppressive activity of DEC1. Expression of these ... resulted in adequate levels of expression. The transcriptional activity of FLAG-BMAL1, and the suppressive activity of FLAG- DEC1, were s imilar to those of intact BMA...
Ngày tải lên : 19/02/2014, 16:20
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