... Functional site of endogenous phospholipase A
2
inhibitor
from python serum
Phospholipase A
2
binding and anti-in¯ammatory activity
Maung-Maung Thwin
1
, Ramapatna ... IC
50
, concentration of the inhibitor that inhibits PLA
2
activity by 50%; PIP, phospholipase inhibitor from python; PLI,
phospholipase A
2
inhibitor; sPLA
2
, secretory phospholipa...
... ligand binding, and the role of
storage proteins, the cupins constitute one of the most
diverse superfamilies of proteins known. They have
been described from all three domains of life [1,2], and
usually ... constitute a large and widespread superfamily of b-barrel proteins
in which a mononuclear metal site is both a conserved feature of the struc-
ture and a source...
... between
helices 3 and 4 at the junction crossover – and the unpairing
of four base-pairs in the centre of the junctions [19]. Detailed
understanding of the functional role of these interactions
would ... well-defined dimer. The cata-
lytic site consisted of two aspartates ) Asp46 and
Asp230 ) which mapped to the catalytic site of RuvC.
Protruding from opposite sides...
... vitro,an
activity characteristic of molecular chaperones and shown here to be shared
by apoferritin and ferritin. This is the first report that apoferritin ⁄ ferritin
may protect cells from stress ... denaturation of citrate synthase in vitro,a
capability shared by apoferritin and ferritin, and con-
fers stress tolerance on transfected mammalian cells.
Artemin and ferritin ma...
... be built, suggesting conservation of ligand and metal
binding sites. Comparison with corresponding sequences from a variety of
organisms shows more than 1 ⁄ 6 of the sequence is completely conserved.
Mutants ... sequence from P. Sher-
manii (MCEE_PROFR) are also labeled.
Fig. 6. Model of MCE-1 structure. View along the length of the
eight-stranded beta-barrel onto the putati...
... Baker
EN (2003) Structural and functional consequences of
binding site mutations in transferrin: crystal structures
of the Asp63Glu and Arg124Ala mutants of the
N-lobe of human transferrin. Biochemistry ... absence or presence of increasing concentrations of unlabeled Ggly. The
ApoTf–Ggly complex was separated from the unreacted Ggly by SDS ⁄ PAGE, and the extent of...
... between the base and
substrate -binding amino acid affected the binding of
dThd and deoxycytidine (dCyd).
Results
We performed site- directed mutagenesis of four active
site residues of Dm-dNK. Residue ... Structural alignment of wild-type Dm-dNK (blue) (PDB ID:
1OE0) and Dm -dNK-E52D (red) illustrating the binding of the feed-
back inhibitor dTTP and the position...
... levels of amino
acid sequence similarity ranging from 45% to 53%, the
architecture of the aglycone -binding site of Zm-p60.1
differs distinctly from that of Bgl4:1 and its homologs.
These findings offer ... analysis of structural and catalytic prop-
erties of the mutants, and simulations of the sub-
strate–enzyme interactions of the wild-type and one
of the mut...
... quantitation of microgram quantities of protein,
utilizing the principle of protein-dye binding. Anal
Biochem 72, 248–254.
38 Segel IH (1993) Enzyme Kinetics: Behavior and Analysis
of Rapid Equilibrium and ... may act
as an allosteric inhibitor of m-NAD-ME [18,21,25],
and the allosteric properties of this isoform may relate
to its particular role in the pathways of mala...
... other h and,
deletion of up to 273 residues from the C-terminal region
of DEC1 (DEC1:1–309, DEC1:1–232 and DEC1:1–139)
had little effect on the suppressive activity of DEC1.
Expression of these ... resulted in
adequate levels of expression. The transcriptional activity
of FLAG-BMAL1, and the suppressive activity of FLAG-
DEC1, were s imilar to those of intact BMA...