... The activation of gelsolin by low pH
The calcium latch is sensitive to calcium but not pH
Emeline Lagarrigue
1
, Diane Ternent
2
, Sutherland K. Maciver
2
, Abdellatif Fattoum
3
, Yves ... induced by lowering pH does not allow a
tight interaction of G4–6 with G-actin.
Discussion
The pH- dependence of the G2/G4–6 interface
Activation of gel...
... catalyses the phosphorylation of a-d-
glucose (Glc) to form glucose 6-phosphate, using
MgATP
2)
as the phosphoryl donor. It is a key regula-
tory enzyme in the pancreatic b-cell [isoform 1 of
human ... states of
hGK [12], the residue contacts that make essential con-
tributions to the binding of Glc to the super-open con-
formation have not been identified. The...
... conserved
motif of Gly–Gly–X–Gly. The second glycine of this
motif (Gly374) is known to make a hydrogen bond
with the pyrophosphate moiety of the FAD of the next
monomer in the tetramer [16]. However, the ... t-test)
(data not shown). Therefore, it is likely that the differ-
ence in the regulation of BmIVD expression between
+
sku
and sku alleles is not r...
... popula-
tion. The inflammatory process is characterized by
infiltration of inflammatory cells into the joints, leading
to the proliferation of fibroblast-like synoviocytes (FLS)
and the destruction of cartilage ... polyarticular joint dis-
ease that primarily affects the small joints of the hands and feet. The
inflammatory process is characterized by infiltration of...
... modulate the fundamental biophysical
protein properties. By applying this methodology to
the study of the kinetics of catalysis by the serine pro-
tease a-CT we were able to statistically correlate the
structurally ... directions. From this analysis the dynamics of the
protein are described by a set of N
)1
normal modes, the k
th
eigenvector (u
k
)ofG gives...
... (EGF),
which bind to dedicated receptors. Upon EGF binding, its
receptor (EGFR) dimerizes, leading to autophosphoryla-
tion of tyrosine residues on the cytoplasmic domain of the
receptor, thereby creating ... time
point of 60 min in order to measure accurately the
synergistic effect.
EGF concentration-dependency of synergistic activation
To investigate the synergisti...
... 2002
The proton signal of His57 Ne2-Ser194 Oc was also
detected at around 14.0 p.p.m. at pH 9.1–6.9. The
His57 Ne2 proton signal disappeared below pH 6.0 due
to the protonation of His57 Ne2. These ... the pK
a
of His210 is 2 pH units higher than that of His57
is the buried charge interaction with Asp113. The shielding
effect of Asp113-His210 by Trp169 was s...
... [11]. In another, noncompetitive
inhibitors with IC
50
values of 0.1 lm were found, but
these were prone to hydrolysis with deactivation half-
lives of 1–2 h. The latter are thought to bind to
NS3pro, ... identified low molecular
mass inhibitor. Our results show that all these inhibitors act by occupying
the substrate-binding site of the protease rather than by an al...
... proteins in the cytosol of the parasite,
leading to the observed toxicity to the cell.
The fact that EhMGL2, which is more active in the
degradation of TFM, is less sensitive than EhMGL1
seems to contradict ... isozymes, and that the degradation
of TFM is less sensitive to alterations of these residues than is the degrada-
tion of physiological su...
... v) Triton X-100, pH 8 [18]. At 30 °C,
the oxidation of the substrate DHO with the quinone cosub-
strate was coupled to the reduction of the chromogen 2,6-di-
chloroindophenol. The K
m
of DHO ... proteins of family 2 must be translo-
cated from the cytosol to the inner membrane of mito-
chondria. The proteins are directed by targeting
sequences, which usuall...