... experiment
we examined the readers’ rating of the same re-
views based on reading single sentences ex-
tracted from these reviews: the last sentence or
the second one. The second sentence could ... frequently appear in
final sentences. Thus, they first focused on the
last sentence of posts as representing the polarity
of the entire posts. Then, they div...
... autoinhibited conformation, the
SH2 domain binds to pY
i
on the C-terminal tail, and
the Src homology 3 (SH3) domain binds to the linker
between the SH2 and kinase domains at the back of
the small ... domain). Regardless
of the slope, the active Src fractions increase during
the Off to On transition, whereas the value of the inac-
tive fraction (s
i
) decreas...
... Ab(L1–40) is the dominant
protein in the inclusion bodies. In contrast, the major
protein in the Ab(M1–42) inclusions was not Ab, but
was the small heat shock protein IbpB (accession num-
ber ... correspondence with the
expected composition, indicating that the peptide is of
the correct sequence and free of contaminating pro-
teins. Five cycles of N-terminal se...
... 1c contributed to the
5¢ nontranslated region of isoform 1 mRNA in which
the common exon 2 contains an initiation codon.
Intronic 5¢ consensus GT was located adjacent to the
3¢ end of each exon. ... around the transcriptional initi-
ation sites.
Determining the translation initiation site of
isoform 2 KLK11
Messenger RNA for isoform 2 contains two initiation
codons near the...
... 6); the G-A transition concurs with the use of
hydroxylamine as the mutagenic agent [14]. Therefore, the
dfp-1 mutation is within the AAVAD motif of the
C-terminal PPC synthetase (CoaB) domain of ... second-half reaction and are part of
the active-site of CoaB; these residues may be directly
involved in binding of one of the substrates, the amino acid
cystein...
... (F340) increases, whereas the
fluorescence intensity at 380 nm (F380) decreases upon
the binding of Ca
2+
. The change in the ratio of
F340 ⁄ F380 determined the change in Ca
2+
concentration
inside ... CaM-binding
IQ domain, did not increase the basal or serotonin-
induced ERK1 ⁄ 2 phosphorylation. The ERK1 ⁄ 2 phos-
phorylation was inhibited in the presence of...
... bFGF in
the absence of FSH (data not shown).
Direct implic ation o f cAMP increase, the second mes-
senger of FSH, in the bFGF regulation was evaluated by
addition to the culture medium of either ... was
performed using the
SECURIA
program from the Packard
Instrument Company (Meriden, CT, USA).
DNA quanti®cation
The DNA content of the cell layer at the end of...
... Microinjection of NLS-containing substrates
in a myoblast. The movie represents the NLS microin-
jection experiment shown in Fig. 6A.
Video S2. Microinjection of NLS-containing substrates
in a myotube. The ... represents the NLS microin-
jection experiment shown in Fig. 6A.
Video S3. Microinjection of NES-NLS-containing sub-
strates in myoblasts. The movie represents...
... (domain A) and the
four domains B, C, D and E. In the case of four-domain
proteins without an N-domain, only domain E (but not
domain D) is present. It should be noted that the function of
domain ... enzymes
containing such a domain. Throughout this paper, domain
E is referred to as SBD, the starch-binding domain.
In a classification of carbohydrate-binding modules, this
s...
... regulation, the blocked green lines indicate negative
regulation, and the dashed lines indicate indirect inhibition. The
black lines indicate substance conversion or transportation flow.
The dashed lines ... GBE50 intake, although
Foxa3 remained upregulated in group HG. In addi-
tion, the decreased expression of Pc in group HG fur-
ther suggested impaired glyconeogenesis. Upr...