Báo cáo khoa học: Structural analysis of the N-glycans of the major cysteine proteinase of Trypanosoma cruzi Identification of sulfated high-mannose type oligosaccharides doc
... (2005) 3803–3815 ª 2005 FEBS 3815
Structural analysis of the N-glycans of the major cysteine
proteinase of Trypanosoma cruzi
Identification of sulfated high-mannose type oligosaccharides
Mariana Barboza
1
, ... Cazzulo JJ
(1993) The reactivity of sera from chagasic patients
against different fragments of cruzipain, the major
cystein proteinase...
... was shown that
the N-glycans of planarian include high mannose -type and pauci-mannose-
type glycans. However, many of the major N-glycans from planarians have
novel structures, as their elution ... map. The diam-
eter of the circles is proportional to the cube root of the detected
amount of PA-glycans. The positions of standard PA-glycans are
indicated by c...
... conformation of loop 99–107 (L2) in the A
protomer as compared to its conformation in the B, C
and D protomers of the tetramer. This loop forms
the roof of the sugar binding pocket of MornigaM
and ... gJRL.
The size and shape of the carbohydrate-binding site
of MornigaM and other JRL essentially depends on
both the conformation of the three loops that delineate...
... the steps in
the biosynthesis of the oligosaccharide portion of the LPS
molecules. The genes lpsA, lic2A and lgtC encode
glycosyltransferase enzymes involved in the addition of
the globoside ... to Hex8)
were of greater abundance in the spectrum of RM6252 than
in the one of RM7290. From ESI-MS data it was also
evident that PCho was not a major substituent in t...
... bond, linkage of the active
site Ser of the protease to the carboxyl group of P
1
by an
ester bond and insertion of the N-terminal part of the RCL
as strand 4 in b-sheet A (s4A) of the serpin. ... cognate
proteases is fuelled by the thermodynamic properties of the
serpin. Accordingly, insertion of the RCL as s4A and
the ensuing structural rearrangements...
... comprising the
N-terminal fragment and the first AAA-cassette of
PEX6 (PEX6N + D1–GAL4-BD). Neither of the cas-
settes alone nor the two AAA-cassettes together or the
N-terminal part together with the ... activation of the reporter gene and thus is suffi-
cient to maintain the interaction with Pex1p. These
findings demonstrate the importance of the first AAA-
cassette...
... assumes that the thiol group of
Cys135 adds to C6 of the pyrimidine ring, favoring
the exit of the aminic group. The subsequent addition
of a water molecule and the expulsion of the active
cysteine ... superposition of the Ca
atoms of one monomer with that of the other members
of the family gives a rmsd of 1.7, 1.3 and 1.4 A
˚
for
models 1RTW, 1UDD an...
... The trend was strengthened by the
inclusion of thermophilic enzymes in the comparison.
Figure 2 shows the percentage of apolar interface,
Table 3. Statistical analysis of the differences in the ... using a set of 512 points located
on a sphere around each atom. The sphere interaction radius
of each atom is equal to the sum of the van der Waals radius
of the...
... respectively, of that of HPRT. This low activity
could be explained by the fact that PRTFDC1 has a Gly in the position of
the proposed catalytic Asp of HPRT. In PRTFDC1, a water molecule at
the position of ... and
regulators of other enzymes in the human nucleotide
metabolism (Table S1). The library can then be seen to
constitute the specific metabolome of the nuc...
... in
NOS, the second electron is provided to the heme-
dioxy species by a bound H
4
B cofactor rather than by
the flavoprotein domain [16]. The H
4
B radical is then
reduced within the enzyme by the ... cytochrome c reduction measurements.
c
The effect of the element is inferred from deletion mutants,
therefore the effects reported in the table are the opposite of the...