... Kinetic analysis of zymogen autoactivation in the presence
of a reversible inhibitor
Wei-Ning Wang, Xian-Ming Pan and Zhi-Xin Wang
National Laboratory of Biomacromolecules, Institute of Biophysics, ... the
autoactivation of zymogens, which is a particular case of the
activation of zymogens. As the autocatalytic activation of
zymogens plays a key...
... in various organ systems. Even in organisms
lacking a brain, such as Caenorhabditis elegans, the
nervous system plays a key role in maintaining energy
balance [1–4]. In more advanced, mammalian ... intracerebroventricular administration
of fatty acid synthase (FAS) inhibitors causes a dra-
matic decrease in food intake and body weight, con-
comitant with an increase in...
... the minimal transcrip-
tionally active fragment and are required simulta-
neously to maintain transcription.
The PHF3 protein was recovered in initial analyses
and also contains a TFS2M domain ... protein domain architectures that
share a canonical bipartite nuclear localization signal
and a PHD domain (Zn finger) at the N-terminal
region. The long isoform also contains transcr...
... recombinant
enzymes free of albumin and any other AAAs. It was
found that there was no fundamental difference in the
mechanisms of inhibition and activation for either the
AAA or esterase activities ... hydrolases that catalyse the
deacylation of N-acyl arylamines [20,21]. Several
AAAs have been identified in mammalian tissues
[22,23]. Certain acryl acylamidases are identical t...
... measure-
ment of the remaining activity towards casein or the
synthetic substrate 2-aminobenzoyl-Ala-Gly-Leu-Ala-4-
nitrobenzylamide (Abz-AGLA-Nba), as described in the
Experimental procedures. Inactivation ... stearothermophilus and its
variants have been based on thermoinactivation meas-
urements assuming that inactivation is determined
by autoproteolysis as a consequence of r...
... depending on the input, interconnections
to other information, and an automatic activity
decay scheme. In addition, the decay scheme is
based on the state of the information, whether it
has reached ... French
Because of the parallel activation of all
meanings of each recognized word in HOPE, the
determination of the phonetic representation of a
recogniz...
... Soaplab
Soaplab (Senger et al., 2003)
2
allows a WSP to
deploy a command line tool as a WS just by writ-
ing a metadata file that describes the parameters
of the tool. Soaplab takes care of the ... translators when they start
translating documents for a new domain. As at
that early stage they still do not have any content
in their TM, having the automatically acquired
T...
... disturbance
Fig. 6. Diagram of the surface of duck d-crystallin (1AUWÆpdb). (A) Each subunit of the tetrameric d-crystallin shows a surface with several convex
and concave areas as indicated by a , ... refraction [1–3]. Thermodynamic stability
for crystallins is essential in maintaining lens transparency
[4]. Determining the mechanism of folding and assembly of
these prote...
... the absence of a B chain [152]. The degrada-
tion of ricin A chain is brefeldin A- insensitive and is
inhibited by the proteasome inhibitor clasto-lactacystin
b-lactone, resulting in the accumulation ... of ricin
A chains. These stabilized ricin A chains are partly
deglycosylated by a peptide–N-glycanase-like activity.
Taken together, these results indicate that...
... Castilla, Alejandra Duarte, Paula Maloberti,
Cristina Paz and Ernesto J. Podesta
´
Department of Biochemistry, School of Medicine, University of Buenos Aires, Argentina
Arachidonic acid (AA) is a fatty ... cells as an
experimental system, we described an alternative
releasing mechanism for AA as a mediator of hormone
action with the participation of an acyl-CoA synthe-
tase (A...