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Báo cáo khoa học: Structural studies of the capsular polysaccharide and lipopolysaccharide O-antigen of Aeromonas salmonicida strain 80204-1 produced under in vitro and in vivo growth conditions docx

Báo cáo khoa học: Structural studies of the capsular polysaccharide and lipopolysaccharide O-antigen of Aeromonas salmonicida strain 80204-1 produced under in vitro and in vivo growth conditions docx

Báo cáo khoa học: Structural studies of the capsular polysaccharide and lipopolysaccharide O-antigen of Aeromonas salmonicida strain 80204-1 produced under in vitro and in vivo growth conditions docx

... Ó FEBS 2004 Structural studies of the capsular polysaccharide and lipopolysaccharide O-antigen of Aeromonas salmonicida strain 80204-1 produced under in vitro and in vivo growth conditions Zhan ... study we have established the structure of the CPS and LPS O-chain polysaccharide of A. salmonicida strain 80204-1 produced under in vitro growth conditions on TSA.Both polysaccharides were shown ... sample of A. salmonicida (data not shown). The proposed structure of CPS and O-chain polysaccharide of A. salmonicida strain 80204-1 is depicted in Fig. 5. The biological significance of these findings...
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Tài liệu Báo cáo khoa học: Structural insights into the substrate specificity and activity of ervatamins, the papain-like cysteine proteases from a tropical plant, Ervatamia coronaria ppt

Tài liệu Báo cáo khoa học: Structural insights into the substrate specificity and activity of ervatamins, the papain-like cysteine proteases from a tropical plant, Ervatamia coronaria ppt

... of binding of E-64 with ervatamin-A and ervatamin-C The modes of binding of E-64 with ervatamin-A and ervatamin-C have been analyzed and compared with the structures of other complexes of the ... to that of ervatamin-B, ervatamin-C [27,35] and other members of the papain family, and is made up of two domains, L and R, with a V-shaped active sitecleft at the interface of the domains (Fig. ... for investigation of the catalytic mechanism and substrate specificity and to understand the differences therein for this class of enzymes. In order to identify the subsites of the ervatamins and...
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Tài liệu Báo cáo khoa học: Comparative studies on the functional roles of N- and C-terminal regions of molluskan and vertebrate troponin-I pdf

Tài liệu Báo cáo khoa học: Comparative studies on the functional roles of N- and C-terminal regions of molluskan and vertebrate troponin-I pdf

... probably induced by streng-thening of the interaction between the structural TnC-binding site and the C-domain of TnC accompanyingCa2+binding to site IV of TnC. In vertebrate tropo-nin, the ... TnC-bind-ing site interacts with the C-domain of TnC in both the relaxed and contractile states, which plays a role in maintaining the structural integrity of the troponincomplex [17,18]. These ... and then run on SDS ⁄ PAGE. Lanes a and d, in the absence of both TnC and Ca2+; lanes b and e, in the presence of TnC and the absence of Ca2+; lanes c and f, in the presence of bothTnC and...
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Báo cáo khoa học: Structural features in the C-terminal region of the Sinorhizobium meliloti RmInt1 group II intron-encoded protein contribute to its maturase and intron ppt

Báo cáo khoa học: Structural features in the C-terminal region of the Sinorhizobium meliloti RmInt1 group II intron-encoded protein contribute to its maturase and intron ppt

... 6097–6100.Supplementary information The following supporting information is available:Fig. S1. Retrohoming in vivo of wild-type intron and C-terminal region mutant derivatives using RmInt1-engineering constructs ... for the insertion of the intron into DNA targets in the orientation that would make it possible to use the nascent leading strandas a primer. These findings suggest that these group II intron intron-encoded ... analyses of LtrA mutants (the IEP of the Ll.ltrB intron of Lactococcus lactis) have suggestedthat the N-terminus of the RT domain is required forprotein interactions with the high-affinity binding...
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Báo cáo khoa học: Structural basis for the erythro-stereospecificity of the L-arginine oxygenase VioC in viomycin biosynthesis docx

Báo cáo khoa học: Structural basis for the erythro-stereospecificity of the L-arginine oxygenase VioC in viomycin biosynthesis docx

... 1). These findings can be explained by the coor-dination of l-arginine in the active site of VioC(Figs 4, 5 and 6B). As the stereochemistry of the Ca-atom is crucial to allow the manifold interactionsbetween ... methylene group in the side chain of l-arginine, as exemplified by the binding and catalyticturnover of l-homoarginine. Concerning l-canavanineturnover by VioC, the modified guanidinium group islikely ... chain of bound hAsn in AsnO points towards the centre of the enzyme complex. The differentsubstrate-binding mode results from conformationalcontrol of the enzyme on the side chain rotamer of the bound...
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Báo cáo khoa học: Structural studies of nucleoside analog and feedback inhibitor binding to Drosophila melanogaster multisubstrate deoxyribonucleoside kinase doc

Báo cáo khoa học: Structural studies of nucleoside analog and feedback inhibitor binding to Drosophila melanogaster multisubstrate deoxyribonucleoside kinase doc

... zalcita-bine) and relate them to the binding of substrate and feedback inhibitors.dCTP and dGTP bind with the base in the substrate site, similarly to the binding of the feedback inhibitor dTTP. All ... deoxynucleo-side kinases with distinct, but overlapping, substrateaffinities. Thymidine kinase 1 (TK1) and deoxycytidinekinase (dCK) are found in the cytosol, and thymidinekinase 2 (TK2) and deoxyguanosine ... 3¢-azido-2¢,3¢-dideoxythymi-dine), zalcitabine (ddC, 2¢,3¢-dideoxycytidine) and brivudin [BVDU, (E)-5-(2-bromovinyl)-2¢-deoxyuri-dine]. The kinetic parameters for these are given in Table 1. When discussing the binding...
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Báo cáo khoa học: NMR studies on the interaction of sugars with the C-terminal domain of an R-type lectin from the earthworm Lumbricus terrestris pot

Báo cáo khoa học: NMR studies on the interaction of sugars with the C-terminal domain of an R-type lectin from the earthworm Lumbricus terrestris pot

... pseudo-threefoldsymmetry of the three subdomains designated a, b and c, and that the protein has two sugar-binding sites in subdomains a and c [24,25]. Therefore, determination of the sugar-binding ability and specificity ... and corresponds tothat of the higher binding site by NMR, even if the sugar-binding ability of one of the two binding sites ismuch higher than that of the other. However, the hemagglutinating ... determined by nonlinearregression fitting of the chemical-shift change versus the sugarconcentration to the binding isotherm describing the binding of oneligand molecule to a single protein site...
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Báo cáo khoa học: Structural insight into the evolutionary and pharmacologic homology of glutamate carboxypeptidases II and III ppt

Báo cáo khoa học: Structural insight into the evolutionary and pharmacologic homology of glutamate carboxypeptidases II and III ppt

... with the occupancy of the S1 pocket by a ligand and the ‘open–close’ transition exploits the hinges on the twosides of the lid made up of Asn540 ⁄ Trp541 and Gly548 (GCPII numbering). In the ... reloca-tion upon inhibitor binding and, in addition, a rota-tion of the Asn257 side chain is necessary toaccommodate the bulky oxodiazolidine ring. Theseobservations are in line with the four-fold ... GCPIII and GCPII, studies addressing the structural and physiological properties of GCPIII are crucial for obtaining a deeperunderstanding of the GCPII ⁄ GCPIII system. In the present study,...
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Báo cáo khoa học: Structural studies of thymidine kinases from Bacillus anthracis and Bacillus cereus provide insights into quaternary structure and conformational changes upon substrate binding pot

Báo cáo khoa học: Structural studies of thymidine kinases from Bacillus anthracis and Bacillus cereus provide insights into quaternary structure and conformational changes upon substrate binding pot

... thymine and the enzyme are to main-chainatoms such that O2 and N3 form hydrogen bonds tomain-chain atoms of residues in the lasso-domain and O4 to main-chain atoms in the a ⁄ b-domain. The methyl ... lasso-domain and a ⁄ b-domain, and the c-phosphate bound to the P-loop as described previ-ously [8,9]. Interestingly, there is no electron density for the inhibitor in the substrate-binding site. Instead, ... nucleo-tide binding in the phosphate-binding site. Bc-TK wascocrystallized with the feedback inhibitor dTTP, hencewe expected it to bind as thymine in the substrate-bind-ing site between the lasso-domain...
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Báo cáo khoa học: Structural basis for the recognition of complex-type biantennary oligosaccharides by Pterocarpus angolensis lectin ppt

Báo cáo khoa học: Structural basis for the recognition of complex-type biantennary oligosaccharides by Pterocarpus angolensis lectin ppt

... In the case of con Aon the other hand, binding of GlcNAc to the )1 subsitedoes not affect affinity [30]. This was attributed to astrained conformation of the disaccharide in the com-plex of ... forGlcNAc binding in the +3 subsite [30], while for the lectin:ligand complexes of PAL this is neutral. In conclusion, the combination of structural biology and thermodynamics has allowed us to obtain ... mix-ture in the binding site of subunit B. Accommodation of the b-configuration in the binding site of PALrequires a change in the rotamer conformation of Glu221 (Fig. 2B). Steric hindrance is...
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