Báo cáo khoa học: Structural studies of the capsular polysaccharide and lipopolysaccharide O-antigen of Aeromonas salmonicida strain 80204-1 produced under in vitro and in vivo growth conditions docx
... Ó FEBS 2004
Structural studies of the capsular polysaccharide and
lipopolysaccharide O-antigen of
Aeromonas salmonicida
strain
80204-1 produced under
in vitro
and
in vivo
growth conditions
Zhan ... study we have established the structure of the CPS
and LPS O-chain polysaccharide of A. salmonicida strain
80204-1 produced under...
... of binding of E-64 with ervatamin-A and
ervatamin-C
The modes of binding of E-64 with ervatamin-A and
ervatamin-C have been analyzed and compared with
the structures of other complexes of the ... to that of ervatamin-B, ervatamin-C [27,35] and
other members of the papain family, and is made up
of two domains, L and R, with a V-shaped active site
cleft at...
... probably induced by streng-
thening of the interaction between the structural TnC-
binding site and the C-domain of TnC accompanying
Ca
2+
binding to site IV of TnC. In vertebrate tropo-
nin, the ... TnC-bind-
ing site interacts with the C-domain of TnC in both
the relaxed and contractile states, which plays a role
in maintaining the structural integrity o...
... 6097–6100.
Supplementary information
The following supporting information is available:
Fig. S1. Retrohoming in vivo of wild-type intron and
C-terminal region mutant derivatives using RmInt1-
engineering constructs ... for the insertion of the intron into DNA targets in the
orientation that would make it possible to use the nascent leading strand
as a primer. These findin...
... 1). These findings can be explained by the coor-
dination of l-arginine in the active site of VioC
(Figs 4, 5 and 6B). As the stereochemistry of the
Ca-atom is crucial to allow the manifold interactions
between ... methylene group in the side chain of
l-arginine, as exemplified by the binding and catalytic
turnover of l-homoarginine. Concerning l-canavanine
turno...
... zalcita-
bine) and relate them to the binding of substrate and feedback inhibitors.
dCTP and dGTP bind with the base in the substrate site, similarly to the
binding of the feedback inhibitor dTTP. All ... deoxynucleo-
side kinases with distinct, but overlapping, substrate
affinities. Thymidine kinase 1 (TK1) and deoxycytidine
kinase (dCK) are found in the cytosol,...
... pseudo-threefold
symmetry of the three subdomains designated a, b and
c, and that the protein has two sugar-binding sites in
subdomains a and c [24,25]. Therefore, determination
of the sugar-binding ability and specificity ... and corresponds to
that of the higher binding site by NMR, even if the
sugar-binding ability of one of the two binding sites is
much hi...
... with
the occupancy of the S1 pocket by a ligand and the
‘open–close’ transition exploits the hinges on the two
sides of the lid made up of Asn540 ⁄ Trp541 and
Gly548 (GCPII numbering). In the ... reloca-
tion upon inhibitor binding and, in addition, a rota-
tion of the Asn257 side chain is necessary to
accommodate the bulky oxodiazolidine ring. These
observa...
... thymine and the enzyme are to main-chain
atoms such that O2 and N3 form hydrogen bonds to
main-chain atoms of residues in the lasso-domain and
O4 to main-chain atoms in the a ⁄ b-domain. The
methyl ... lasso-domain and a ⁄ b-domain, and
the c-phosphate bound to the P-loop as described previ-
ously [8,9]. Interestingly, there is no electron density for
the inhibit...
... In the case of con A
on the other hand, binding of GlcNAc to the )1 subsite
does not affect affinity [30]. This was attributed to a
strained conformation of the disaccharide in the com-
plex of ... for
GlcNAc binding in the +3 subsite [30], while for the
lectin:ligand complexes of PAL this is neutral.
In conclusion, the combination of structural biology
a...