... Chaperone activity of recombinant maize chloroplast protein synthesis
elongation factor, EF-Tu
Damodara Rao
1
, Ivana Momcilovic
1
, Satoru ... hypothesized that, in maize, this protein may show
chaperone activity similar to prokaryotic EF-Tu [5,9–11].
Fig. 6. Effect of recombinant m aize pre -EF-Tu ( EF-Tu) on the activity
of citra te synthase ... prot...
... scattering can be used as a measure of
effectiveness of the chaperone. Using this assay we tested the
relative activity of the two wheat proteins in suppressing
aggregation of MDH. As shown in Fig. 5, ... its chaperone
activity, and likewise, data on activity of the class II pro-
teins is very limited. We prepared purified, recombinant
TaHsp16.9C-I and TaHsp17.8C-II and find...
... Rockford, IL, USA). Bands of GST-fusion proteins
were detected by staining the PVDF membranes with CBB.
In vitro binding assay using recombinant
proteins
Ten micrograms of GST (negative control) ... epidermal growth factor, suggesting involvement
of calpain 7 in the ESCRT system [28].
On the basis of the resolved 3D structure, the MIT
domain of VPS4 forms three-helix bundles. ES...
... the
existence of several types of hemin conformation in the
protein pocket is consistent with this non a-specific
production of biliverdin. We expected that DmDHO
would produce the c-isomer of biliverdin ... spectrum of hexa-
coordinated heme protein. The absorption spectrum of the
ferric complex was not influenced by changing the pH of the
solution. Interestingly, an EPR study...
... for the
function of BchI among the various functions of AAA
+
proteins. The AAA
+
proteins represent one type of
molecular chaperones and their function is to control the
fate of proteins or DNA. ... This is done by facilitating protein
folding and unfolding, assembly and disassembly of protein
complexes, degradation of protein, replication and tran-
scription of DNA, etc....
... the
mechanism of action of chymotrypsin: the role of the low barrier
hydrogen bond. Biochemistry 36, 4576–4584.
46. Warshel, A. (1998) Electrostatic origin of the catalytic power of
enzymes and the role of ... general features of the
recently determined X-ray structure of human BuChE [7,8].
In particular, most of the essential features of the catalytic
site (i.e. a catalyt...
... refer to amino-acid residues of the C. albicans protein. (B) Alignment of
the catalytic centre of the recombinantly expressed C. albicans DHODH and amino-acid sequences of different DHODH family ... of recombinant DHODH should expedite discov-
ery of more potent agents for growth control
strategies in C. albicans, and permit the screening of
a large number of compounds, the...
... Devices).
Results
Identification of histone proteins in the hemocytes
In the course of an initial proteomic inve stigation of shrimp
immune function, simple one-dimensional SDS/PAGE
analysis of the hemocytes soluble proteins ... the results of a proteomic investigation
of hemocyte antimicrobial peptides of the P acific white
shrimp, L. vannamei, and the r ole of histones as pote...
... supernatant.
Fig. 6. Autophosphorylation of recombinant Ndk and mutant proteins.
(A) Ndk and mutant proteins (1 lg) were incubated in the presence of
10 lCi of [c-
32
P]ATP in 20 lL of reaction volume. The reaction ... of 2 lL of 10% SDS. The samples were boiled
for 10 min and separated by 15% SDS/PAGE. Analysis
was by autoradiography.
Enzymatic activity of Ndk
Enzymatic ac...
... region, one copy of a
double stranded oligonucleotide spanning the region from
)70 to )36 was fused upstream of pTATALUC. Evaluation
of reporter activity following transfection of A6 cells
revealed ... detected in bone, cartilage and in soft tissues such
as he art, kidney, and lung in a variety of species [4,6,7].
MGP is also secreted in vitro by a number of cell lines of
diff...