Báo cáo khoa học: Mutational analysis of functional domains in Mrs2p, the mitochondrial Mg2+ channel protein of Saccharomyces cerevisiae ppt

Báo cáo khoa học: Mutational analysis of functional domains in Mrs2p, the mitochondrial Mg2+ channel protein of Saccharomyces cerevisiae ppt

Báo cáo khoa học: Mutational analysis of functional domains in Mrs2p, the mitochondrial Mg2+ channel protein of Saccharomyces cerevisiae ppt

... 2006) doi:10.1111/j.1742-4658.2006.05157.x The nuclear gene MRS2 in Saccharomyces cerevisiae encodes an integral protein (Mrs2p) of the inner mitochondrial membrane. It forms an ion channel mediating in ux of Mg 2+ into mitochondria. ... feature of Mrs2p is the existence of two transmembrane -domains and a short connect- ing sequence of about 7–8 amino acids. Thi...
Ngày tải lên : 23/03/2014, 10:21
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Tài liệu Báo cáo khoa học: Mutational analysis of plasminogen activator inhibitor-1 Interactions of a-helix F and its neighbouring structural elements regulates the activity and the rate of latency transition pdf

Tài liệu Báo cáo khoa học: Mutational analysis of plasminogen activator inhibitor-1 Interactions of a-helix F and its neighbouring structural elements regulates the activity and the rate of latency transition pdf

... linkage of the active site Ser of the protease to the carboxyl group of P 1 by an ester bond and insertion of the N-terminal part of the RCL as strand 4 in b-sheet A (s4A) of the serpin. Consequently, the ... behaviour of the serpin. Complex formation between serpins and their cognate proteases is fuelled by the thermodynamic properties of the serpin. Acc...
Ngày tải lên : 20/02/2014, 11:20
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Báo cáo khoa học: Mutational analysis of substrate recognition by human arginase type I ) agmatinase activity of the N130D variant pot

Báo cáo khoa học: Mutational analysis of substrate recognition by human arginase type I ) agmatinase activity of the N130D variant pot

... intermediate of NO synthesis [26]. On the other hand, in contrast with Asn130 in arginase I, the corres- ponding Asn149 of arginase II was not found to be a ligand for the a-carboxylate group of the transition state ... ligand for the a-carboxyl group of arginine in arginase I [9,23,24]. On this basis, direct repulsion of the negatively charged Asp130 would explain...
Ngày tải lên : 16/03/2014, 12:20
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Báo cáo khoa học: Mutational analysis of the C-domain in nonribosomal peptide synthesis pot

Báo cáo khoa học: Mutational analysis of the C-domain in nonribosomal peptide synthesis pot

... bound in the binding poc ket o f a nother e nzyme molecule within the active homomultimer. This could explain the distance in sequence homology with NRPSs C -domains displayed in the alignment of the ... [5]. Furthermore, a chromosomal point mutatio n in srfA-B, changing a D to A in the His-motif (italic) of the corresponding Asp domain of the surfactin synthe...
Ngày tải lên : 17/03/2014, 11:20
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Báo cáo khoa học: Mutational analysis of the preferential binding of human topoisomerase I to supercoiled DNA pot

Báo cáo khoa học: Mutational analysis of the preferential binding of human topoisomerase I to supercoiled DNA pot

... shortened linker in the mutant enzyme destabilized the interaction between the COOH-terminal and core domains, enabling the COOH-terminal domain of one protein to occupy its binding site in the core ... second DNA-binding site on the protein. Fourth, clusters of basic residues in core subdomain III, and the linker on the side of the protein distal from the...
Ngày tải lên : 29/03/2014, 22:21
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Báo cáo khoa học: Mutational analysis of a type II thioesterase associated with nonribosomal peptide synthesis pdf

Báo cáo khoa học: Mutational analysis of a type II thioesterase associated with nonribosomal peptide synthesis pdf

... removal of short acyl chains originating from aberrant decarboxylation of chain extender units from the thiol moiety of the 4¢-Ppant cofactors of acyl carrier proteins [15,16]. In NRPSs there is ... acetyl-S-Ppant-PCP. Interestingly, after 5–10 min of incubation at 37 °C, no further hydrolysis of the remain- ing substrate was observed, indicating clear instability of thi...
Ngày tải lên : 30/03/2014, 13:20
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Tài liệu Báo cáo khoa học: Control analysis as a tool to understand the formation of the las operon in Lactococcus lactis doc

Tài liệu Báo cáo khoa học: Control analysis as a tool to understand the formation of the las operon in Lactococcus lactis doc

... all the genes involved in the pathway in the same operon; the cell can then respond quickly to changes in the environ- ment by changing the expression of only a few genes and using the protein- synthesizing ... by the second gene in the las operon (Fig. 1). In order to obtain strains with increased PK activity an additional copy of the pyk gene was recombine...
Ngày tải lên : 19/02/2014, 17:20
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Báo cáo khoa học: Ibuprofen binding to secondary sites allosterically modulates the spectroscopic and catalytic properties of human serum heme–albumin doc

Báo cáo khoa học: Ibuprofen binding to secondary sites allosterically modulates the spectroscopic and catalytic properties of human serum heme–albumin doc

... December 2010) doi:10.1111/j.1742-4658.2010.07986.x The ibuprofen primary binding site FA3–FA4 is located in domain III of human serum albumin (HSA), the secondary clefts FA2 and FA6 being sited in domains I and II. Here, the thermodynamics of ... effects, combining the actions of ibuprofen and warfarin [8]. It is worth noting that the modular architecture of HSA allow...
Ngày tải lên : 06/03/2014, 01:20
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Báo cáo khoa học: Eukaryotic class 1 translation termination factor eRF1 ) the NMR structure and dynamics of the middle domain involved in triggering ribosome-dependent peptidyl-tRNA hydrolysis pptx

Báo cáo khoa học: Eukaryotic class 1 translation termination factor eRF1 ) the NMR structure and dynamics of the middle domain involved in triggering ribosome-dependent peptidyl-tRNA hydrolysis pptx

... rearrangement of the protein during the docking to the binding site on the ribosome or other ligands. Strikingly, the second most flexible part of the protein (if one does not take into account the N-termi- nal ... coli in vivo can be explained by their competitive inhibition at the ribosome-binding site [32]. Together, the M and C domains of human eRF1, in t...
Ngày tải lên : 07/03/2014, 05:20
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Báo cáo khoa học: Retrocyclin RC-101 overcomes cationic mutations on the heptad repeat 2 region of HIV-1 gp41 ppt

Báo cáo khoa học: Retrocyclin RC-101 overcomes cationic mutations on the heptad repeat 2 region of HIV-1 gp41 ppt

... number of RC-101 binding conforma- tions. Docking of RC-101 to HR1 alone did not result in a strong binding energy [Fig. 2]. Conversely, the minimum energy of binding to HR2 is predominantly lower ... binding of RC-101 to HR1 and the specific binding to HR2 seen in this work. RC-101 binds reversibly but with high affinity to glycoproteins and associates with the cellular lipid...
Ngày tải lên : 07/03/2014, 05:20
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