Báo cáo khoa học: Puriﬁcation and cloning of a Delta class g

Báo cáo khoa học: Puriﬁcation and cloning of a Delta class glutathione S-transferase displaying high peroxidase activity isolated from the German cockroach Blattella germanica pptx

... Merck and Co., Inc. Journal compilation ª 2007 FEBS 1803 Puriﬁcation and cloning of a Delta class glutathione S-transferase displaying high peroxidase activity isolated from the German cockroach Blattella ... 2007) doi:10.1111/j.1742-4658.2007.05728.x A highly active glutathione S-transferase was puriﬁed from adult German cockroaches, Blattel...

Ngày tải lên : 23/03/2014, 09:21

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Tài liệu Báo cáo khoa học: Puriﬁcation and characterization of a sialic acid speciﬁc lectin from the hemolymph of the freshwater crab Paratelphusa jacquemontii pdf

... [34]. An evaluation of the literature revealed that purification of lectin from the hemolymph of crustaceans was most successful by affinity chromatography as it gave a higher fold of purification and percentage ... Purification and characterization of a sialic acid specific lectin from the hemolymph of the freshwater crab Paratelphusa jacquemontii Maghil Deni...

Ngày tải lên : 21/02/2014, 00:20

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Tài liệu Báo cáo khoa học: Puriﬁcation and characterization of a membrane-bound enzyme complex from the sulfate-reducing archaeon Archaeoglobus fulgidus related to heterodisulﬁde reductase from methanogenic archaea pdf

... with an apparent molecular mass of 53 kDa appears as a double band in unboiled samples (lanes A1 and B1). Table 1. N-Terminal sequences of the polypeptides of the puriﬁed enzyme. N-Terminal sequen ... 3 bp). The region 81–65 bp upstream of t he start c odon of AF499 was identiﬁed as an archaeal promoter element by seque nce analysis. The sequ ence AAAGGTTAATATA shows a...

Ngày tải lên : 21/02/2014, 03:20

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Báo cáo khoa học: Puriﬁcation and properties of a new S-adenosyl-Lmethionine:ﬂavonoid 4¢-O-methyltransferase from carnation (Dianthus caryophyllus L.) pot

... in the biosynthesis of methylated dianthramide-derivatives, the carnation phyto- alexins. However, no data are available regarding the role of this enzymatic activity in the biosynthesis of methylated phenols ... activity was weak and did not change statistically along the 72 h of the observation period. A statistically signiﬁcant increase of the F 4¢-OMT acti...

Ngày tải lên : 08/03/2014, 08:20

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Tài liệu Báo cáo khoa học: Puriﬁcation and characterization of glutamate N-acetyltransferase involved in citrulline accumulation in wild watermelon doc

... number AI563351) was used to design four GAT-speciﬁc primers; CLGAT 5a (5¢-GGCATCAACAT- CACAAGCAACAAGTGCAAG-3¢), CLGAT5b (5¢-TCCT- CCATCAATCTGCTTCCATGGACCATC-3¢), CLGAT 3a (5¢-GCTGTGGCTACGAATGAGGCCGCC-3) ... FEBS Puriﬁcation and characterization of glutamate N-acetyltransferase involved in citrulline accumulation in wild watermelon Kentaro Takahara, Kinya Akashi and Akiho Yokota Gra...

Ngày tải lên : 20/02/2014, 03:20

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Báo cáo khoa học: Puriﬁcation and characterization of zebraﬁsh hatching enzyme – an evolutionary aspect of the mechanism of egg envelope digestion pot

... forward: 5¢-CTGAACT TCTCTACACACTGAGG-3¢, reverse: 5¢-CCTTATCACC ATCACCTCACTTC-3¢; ZHE2 forward: 5¢-CTCCACACA CTGAGACTAAATGG-3¢, reverse: 5¢-GGAAATAAGAG CACGTACTGTGG-3¢. The cycle number of PCR was ... using a sense primer, 5¢-CATATGAATGCTCTCATCTG CGAGG ACA-3¢, containing a 5¢ NdeI site and start methionine resi- due, and an antisense primer, 5¢-GGATCCTAGTGATG GTGATGGTGGCATCCATACAG...

Ngày tải lên : 07/03/2014, 04:20

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Báo cáo khoa học: Puriﬁcation and characterization of the cysteine proteinases in the latex of Vasconcellea spp. ppt

... translation showed that they hold the characteristic features of all known papain -class cysteine proteinases, and a phylogenetic analysis revealed the existence of several papain and chymopapain ... common ancestor of the genera Carica and Vasconcellea already contained at least two different cysteine proteinases in its latex (papain and chymopapain). After the div...

Ngày tải lên : 07/03/2014, 11:20

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Báo cáo khoa học: Puriﬁcation and characterization of cathepsin B-like cysteine protease from cotyledons of daikon radish, Raphanus sativus docx

... Hiroko Saika, Keizo Yuasa and Masami Nagahama Department of Biological Science and Technology, University of Tokushima Graduate School, Japan Genome analysis has indicated that plants, like animals, ... most of the activity was lost. CBCP had optimal activity at 40 °C. The activity of the puriﬁed enzyme was also examined against various synthetic substrates and compa...

Ngày tải lên : 16/03/2014, 04:20

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Báo cáo khoa học: Puriﬁcation and characterization of Helicobacter pylori arginase, RocF: unique features among the arginase superfamily doc

... arginase and H. pylori arginase- containing extracts with divalent cations Mammalian arginases require manganese for optimal catalytic activity, although some of these arginases may be activated ... puriﬁed and characterized, most are from the genus Bacillus; like eukaryotic arginases, these prokaryotic arginases were found to have an optimal catalytic activity with manganese an...

Ngày tải lên : 16/03/2014, 16:20

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Báo cáo khoa học: Puriﬁcation and characterization of NTPDase1 (ecto-apyrase) and NTPDase2 (ecto-ATPase) from porcine brain cortex synaptosomes doc

... changed their afﬁnity towards ATP and ADP [11–15]. NTPDase1 differs from NTPDase2 in respect to reaction products of ATP hydrolysis and in the ratio of the rate of ATP hydrolysis to the rate of ... conditions and in a 10% acrylamide SDS gel (Figs 1, 2A) . Ion exchange chromatography on a MonoQ HR 5/5 column was a crucial step of puriﬁcation and separation of...

Ngày tải lên : 17/03/2014, 10:20

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