Báo cáo khoa học: Fast set-up of doxycycline-inducible protein expression in human cell lines with a single plasmid based on Epstein– Barr virus replication and the simple tetracycline repressor ppt
... Fast set-up of doxycycline-inducible protein expression in
human cell lines with a single plasmid based on Epstein–
Barr virus replication and the simple tetracycline repressor
Markus Bach
1
, ... origin of replication for
stable and efficient episomal propagation in human cell lines, a cassette for
continuous expression of...
... effect in human galactokinase.
(A) The variation of k
cat
with mole fraction of deuterium oxide. These
values were obtained in an experiment in which the concentration of
ATP was varied and galactose ... galactose was maintained at a saturating level
(5 m
M
). Similar results were obtained when ATP was the saturating
ligand and galactose concentration was varied (n...
... pneumoniae strain contains only a single NADH oxi-
dizing enzyme, one can expect a different regulation of the
nuo-operon in K. pneumoniae M 5a1 . Based on preliminary
sequence analysis of the promoter ... upstream regions of the
K. pneumoniae nuoA gene and determination of the NADH
oxidation rate in the K. pneumoniae fnr mutant strain, we
speculate that in...
... protein and from each other. The main effect
seen was in the turnover, k
cat
, and in the specificity
constant, k
cat
⁄ K
m
. Again, this contrasts with disease-
causing mutations in galactokinase ... protease inhibi-
tors in the solutions used during purification.
Kinetic analysis of disease-causing mutations
The steady-state kinetic parameters of each of the...
... investigate
whether the observations made thus far are based on a
global inhibition of the sodium pump, the uptake of
86
Rb
+
into endothelial cells was determined as a function of the
ouabain concentration. ... serum components might induce MAP
kinase activation independently of ouabain.
Ouabain was added to the cells at various concentra-
tions in ECGM-SF, and...
... consist of two domains, a large a ⁄ b
domain and a small a domain with the catalytic site at
the interface of the two domains [4,5]. HAPs can initi-
ate hydrolysis of phytate at either the C
3
(EC ... The k
cat
⁄ K
m
values increase from G1P over
PhyK to AppA by a factor of 2200. The confor-
mational changes of AppA upon substrate binding
facilitate a f...
... concentra-
tion was determined using the Coomassie Plus Protein Assay
Reagent (Pierce) with bovine serum albumin as the standard.
Expression and purification of recombinant protein was
confirmed by SDS ⁄ PAGE ... characterization of IVD deficiency in metazoa.
Considering that IVD acts in the third step of leucine degradation and the
sku mutant accumulates branched-c...
... could indicate that the corre-
sponding hydroxyl groups are involved in protein
binding.
The results for the linebroadening measurements of
protons H 1a in the alpha and beta configurations,
aHa1 and ... com-
monly appeared in alternate positions in the carbohy-
drate oligomers chain (above and below the plain of
the sugar rings) even if the initial calcula...
... in am-
matory disease that occurs in about 1% of the popula-
tion. The in ammatory process is characterized by
in ltration of in ammatory cells into the joints, leading
to the proliferation of ... in ltration of in ammatory cells
into the joints, leading to proliferation of synoviocytes and destruction of
cartilage and bone. In RA synovial tissue, the in...
... VVA1 (VVA2
beads) was incubated with increasing amounts of
VVA2, and visualization on an SDS ⁄ PAGE gel showed
that the adsorbed VVA2 had oligomerized. As the
amount of VVA2 in the reaction was ... Characteristics of volvatoxin A1 (VVA1) structure. (A) Alignment of the deduced amino acid sequence of VVA1 N-terminal domain
(VVA1-NTD) and VVA1 C-terminal domain (VVA...