Báo cáo khoa học: Fast set-up of doxycycline-inducible protein expression in human cell lines with a single plasmid based on Epstein– Barr virus replication and the simple tetracycline repressor ppt

Báo cáo khoa học: Fast set-up of doxycycline-inducible protein expression in human cell lines with a single plasmid based on Epstein– Barr virus replication and the simple tetracycline repressor ppt

Báo cáo khoa học: Fast set-up of doxycycline-inducible protein expression in human cell lines with a single plasmid based on Epstein– Barr virus replication and the simple tetracycline repressor ppt

... Fast set-up of doxycycline-inducible protein expression in human cell lines with a single plasmid based on Epstein– Barr virus replication and the simple tetracycline repressor Markus Bach 1 , ... origin of replication for stable and efficient episomal propagation in human cell lines, a cassette for continuous expression of...
Ngày tải lên : 23/03/2014, 09:21
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Báo cáo khoa học: Functional analysis of disease-causing mutations in human galactokinase potx

Báo cáo khoa học: Functional analysis of disease-causing mutations in human galactokinase potx

... effect in human galactokinase. (A) The variation of k cat with mole fraction of deuterium oxide. These values were obtained in an experiment in which the concentration of ATP was varied and galactose ... galactose was maintained at a saturating level (5 m M ). Similar results were obtained when ATP was the saturating ligand and galactose concentration was varied (n...
Ngày tải lên : 08/03/2014, 02:20
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Báo cáo khoa học: Oxygen control of nif gene expression in Klebsiella pneumoniae depends on NifL reduction at the cytoplasmic membrane by electrons derived from the reduced quinone pool doc

Báo cáo khoa học: Oxygen control of nif gene expression in Klebsiella pneumoniae depends on NifL reduction at the cytoplasmic membrane by electrons derived from the reduced quinone pool doc

... pneumoniae strain contains only a single NADH oxi- dizing enzyme, one can expect a different regulation of the nuo-operon in K. pneumoniae M 5a1 . Based on preliminary sequence analysis of the promoter ... upstream regions of the K. pneumoniae nuoA gene and determination of the NADH oxidation rate in the K. pneumoniae fnr mutant strain, we speculate that in...
Ngày tải lên : 17/03/2014, 10:20
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Báo cáo khoa học: Functional analysis of disease-causing mutations in human UDP-galactose 4-epimerase pot

Báo cáo khoa học: Functional analysis of disease-causing mutations in human UDP-galactose 4-epimerase pot

... protein and from each other. The main effect seen was in the turnover, k cat , and in the specificity constant, k cat ⁄ K m . Again, this contrasts with disease- causing mutations in galactokinase ... protease inhibi- tors in the solutions used during purification. Kinetic analysis of disease-causing mutations The steady-state kinetic parameters of each of the...
Ngày tải lên : 23/03/2014, 11:20
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Báo cáo khóa học: Ouabain stimulates endothelin release and expression in human endothelial cells without inhibiting the sodium pump Robert Saunders and Georgios Scheiner-Bobis pptx

Báo cáo khóa học: Ouabain stimulates endothelin release and expression in human endothelial cells without inhibiting the sodium pump Robert Saunders and Georgios Scheiner-Bobis pptx

... investigate whether the observations made thus far are based on a global inhibition of the sodium pump, the uptake of 86 Rb + into endothelial cells was determined as a function of the ouabain concentration. ... serum components might induce MAP kinase activation independently of ouabain. Ouabain was added to the cells at various concentra- tions in ECGM-SF, and...
Ngày tải lên : 30/03/2014, 13:20
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Tài liệu Báo cáo khoa học: Crystal structure of Klebsiella sp. ASR1 phytase suggests substrate binding to a preformed active site that meets the requirements of a plant rhizosphere enzyme doc

Tài liệu Báo cáo khoa học: Crystal structure of Klebsiella sp. ASR1 phytase suggests substrate binding to a preformed active site that meets the requirements of a plant rhizosphere enzyme doc

... consist of two domains, a large a ⁄ b domain and a small a domain with the catalytic site at the interface of the two domains [4,5]. HAPs can initi- ate hydrolysis of phytate at either the C 3 (EC ... The k cat ⁄ K m values increase from G1P over PhyK to AppA by a factor of  2200. The confor- mational changes of AppA upon substrate binding facilitate a f...
Ngày tải lên : 16/02/2014, 09:20
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Tài liệu Báo cáo khoa học: Molecular defect of isovaleryl-CoA dehydrogenase in the skunk mutant of silkworm, Bombyx mori ppt

Tài liệu Báo cáo khoa học: Molecular defect of isovaleryl-CoA dehydrogenase in the skunk mutant of silkworm, Bombyx mori ppt

... concentra- tion was determined using the Coomassie Plus Protein Assay Reagent (Pierce) with bovine serum albumin as the standard. Expression and purification of recombinant protein was confirmed by SDS ⁄ PAGE ... characterization of IVD deficiency in metazoa. Considering that IVD acts in the third step of leucine degradation and the sku mutant accumulates branched-c...
Ngày tải lên : 18/02/2014, 04:20
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Tài liệu Báo cáo khoa học: Molecular determinants of ligand specificity in family 11 carbohydrate binding modules – an NMR, X-ray crystallography and computational chemistry approach doc

Tài liệu Báo cáo khoa học: Molecular determinants of ligand specificity in family 11 carbohydrate binding modules – an NMR, X-ray crystallography and computational chemistry approach doc

... could indicate that the corre- sponding hydroxyl groups are involved in protein binding. The results for the linebroadening measurements of protons H 1a in the alpha and beta configurations, aHa1 and ... com- monly appeared in alternate positions in the carbohy- drate oligomers chain (above and below the plain of the sugar rings) even if the initial calcula...
Ngày tải lên : 18/02/2014, 17:20
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Tài liệu Báo cáo khoa học: Molecular aspects of rheumatoid arthritis: chemokines in the joints of patients pdf

Tài liệu Báo cáo khoa học: Molecular aspects of rheumatoid arthritis: chemokines in the joints of patients pdf

... in am- matory disease that occurs in about 1% of the popula- tion. The in ammatory process is characterized by in ltration of in ammatory cells into the joints, leading to the proliferation of ... in ltration of in ammatory cells into the joints, leading to proliferation of synoviocytes and destruction of cartilage and bone. In RA synovial tissue, the in...
Ngày tải lên : 18/02/2014, 18:20
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Tài liệu Báo cáo khoa học: Inhibitory effects of nontoxic protein volvatoxin A1 on pore-forming cardiotoxic protein volvatoxin A2 by interaction with amphipathic a-helix doc

Tài liệu Báo cáo khoa học: Inhibitory effects of nontoxic protein volvatoxin A1 on pore-forming cardiotoxic protein volvatoxin A2 by interaction with amphipathic a-helix doc

... VVA1 (VVA2 beads) was incubated with increasing amounts of VVA2, and visualization on an SDS ⁄ PAGE gel showed that the adsorbed VVA2 had oligomerized. As the amount of VVA2 in the reaction was ... Characteristics of volvatoxin A1 (VVA1) structure. (A) Alignment of the deduced amino acid sequence of VVA1 N-terminal domain (VVA1-NTD) and VVA1 C-terminal domain (VVA...
Ngày tải lên : 19/02/2014, 06:20
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