... motif of FhaC. The VRGY
tetrad is highly conserved in the TpsB family and, in FhaC, it is located at
the tip of L6 reaching the periplasm. Replacement by Ala of the invariant
Arg dramatically affects ... against a major con-
formational change of the loop. Structural data are available in the Protein Data Bank under the accession numbers 2QDZ (FhaC
WT
) a...
... Consequently, the PaaF and PaaH proteins appear to
catalyse reactions in the downstream part of phenylacetate
degradation. In the absence of the PaaF and PaaH proteins,
catabolism of phenylacetate is ... suggests a role for the PaaG and PaaZ
proteins early in the pathway. The other major finding is
that C
6
dicarboxylic acids formed later in the pathway are
deri...
... Schwartzberg
National Human Genome Research Institute, National Institutes of Health, Bethesda, MD, USA
Introduction
Among the key players in intracellular signaling in lym-
phocytes are the Tec family kinases ... cascade of signal-
ing events initiated by the activation of the Src-family
kinase Lck, which phosphorylates immunoreceptor
tyrosine activation motifs on the int...
... Discussion
Sulfonimides as inhibitors of RNase A
We began by determining the ability of three backbone
analogs of RNA to inhibit catalysis by RNase A.
These analogs have a simple polyanionic backbone
with neither a ... USA
Introduction
Upon catalyzing the cleavage of RNA, RNases operate
at the crossroads of transcription and translation.
Bovine pancreatic RNase A (EC...
... PCR with GAGCGG
CATATGGA
AATCAAACCGAAGGTTCGCGA and GAGCGG
CATA
TGGAAATCAAACCGAAGGTTCGCGA as the forward
and reverse oligonucleotide primers, respectively. The
primers were designed to introduce ... coordi-
nated by an oxygen donor group derived from the
carboxylate side chain of either a glutamate or an
aspartate. The apparent conflict of this finding with
the absence of a c...
... staphy-
lococcal adhesins, comprising an N-terminal region that binds fibrinogen
and elastin, and a C-terminal domain that interacts with fibronectin. The
C-terminal domain of fibronectin-binding ... FEBS
Monoclonal antibodies against FnBRs of FnBPB
A panel of mouse mAbs was produced against the
recombinant repetitive region of FnBPB. Analysis of
mAbs binding to the recombina...
... are
parasitic protozoa belonging to the order Kinetoplast-
ida. They are causative agents of several highly disab-
ling and often fatal diseases, including African sleeping
sickness, Chagas disease ... exhibits another variation in the first steps
of the pathway. C18 FAs are elongated to C20, and
then a D8 desaturase produces the same kind of C20
FAs that will be the substr...
... isolated
from strains retaining CrtO, this carotene appears to
be in the all-trans form.
A characteristic difference in the carotenoid content
of the PS1-less mutant and the derived strain lacking
echinenone ... Koyama Y, Takii T, Saiki K & Tsukida K (1983) Con-
figuration of the carotenoid in the reaction center of
photosynthetic bacteria. 2. Comparison of the...
... been
partially induced against the linear isoform of the HNE
peptide. Although the binding pattern may be somewhat
blurred by these antibodies and by the polyclonal nature of
the sera, the importance ... [32].
Evenasmallsidechaininposition388wouldresultinsteric
hindrance with the main-chain nitrogen atom of K389,
damaging the shape of the loop. The above spatial...