... al.[19].Analyticalenzymes
and enzyme substrates were specified in previous papers
[15–18]. Al l other chemicals were of reagent grade and
obtained from Sigma and F luka.
Preparation of apo-
Cc
StP and apo-
Ec
MalP
Screening ... citrate and
0.1
M
cysteine hydrochloride, in short, the resolution buffer.
Various pH values between 5.0 and 8.0 were tested, and a
pH of 7.0 was chosen (...
... Structure function studies of E. cloacae IDPC (Eur. J. Biochem. 270) 2325
indolepyruvate (triplets of 5-H and 6-H at 7.04 and
7.12 p.p.m., doublets of 4-H and 7-H at 7.39 and
7.42 p.p.m and the ... and
spin systems of the indole moiety (triplets of 5-H and 6-H at
7.12 and 7.18 p.p.m., doublets of 4-H and 7-H at 7.43 and
7.75 p.p.m and the singlet of 2-H at 7.81 p.p.m. w...
... ourselves to fea-
tures which are binary functions of the history and
aAssumption (4) has not been discussed very much,
but our investigation with humans showed that it is
true in more than ... listed in
Tables 1 and 2. Each row in Table 1 contains a
feature type, feature values, and an experimental
result that will be explained later. Each feature
consists of a type and a val...
... switch
I. Dı
´
az-Moreno, J. M. Garcı
´
a-Heredia, M. Salzano, M. Orza
´
ez,
E. Pe
´
rez-Paya
´
, M. Teixeira, A. Dı
´
az-Quintana and M. A. De la
Rosa
Instituto de Bioquı
´
mica Vegetal y Fotosı
´
ntesis, ... Rodrı
´
guez-Rolda
´
n V., Garcı
´
a-Heredia J.M., Navarro J.A., De
la Rosa M.A., Herva
´
s M. (2008) Biochemistry 47, 12371–
12379
[2]...
...
with tags and features for training a
conditional random field model. At run-
time, the model is used to extracting
translation candidates for a given term.
Preliminary experiments and evaluation ... BIO notation.
3.1.1 Retrieving and tagging snippets. We use a
list of randomly selected source and target terms as
seed data (e.g., Wikipedia English titles and their
131
Ch...
... node (and all the nodes
above it).
Tree C for Diseases is also homogeneous; the child
nodes are a kind of (hyponym of) the disease at the par-
ent node: Neoplasms (C04) is a kind of Disease C and
Hamartoma ... is, C (a disease) , C10
(Nervous System Diseases), C10.228 (Central Nervous
1
http://www.nlm.nih.gov/mesh/meshhome.html; the work
reported in this paper uses MeSH 2001.
System D...
... three antiparallel strands (1, 2 and 7) and
strand 6, which is parallel to strand 2. b-Strands are
located between the four a-helices (a1, 278–294; a2,
305–313; a4, 374–381; and a5, 397–405), with ... upon
request.
Expression and purification of human RFs
Wild-type human eRF1, its mutants and eRF3c containing
His
6
-tags at the C-termini were produced in
E. coli BL21(DE3), and...
... dependency-grammar-based
parsers (Tapanainen and J¨arvinen, 1997; Br¨oker et al., 1994;
Lesmo and Lombardo, 2000), where functional labels are
treated as first-class citizens as relations between words, and
recent work on ... standard, remain
unattached in the output tree; and nodes contained
in the gold standard for which no match could be
found in the parser output. Our approach foll...
... residues and solvent molecules engaged in
ligand recognition and stabilization are drawn as balls -and- sticks and
spheres, respectively. The major interactions established between
the DHAP inhibitor and ... cross-rotation
and translation functions in the 10–4 A
˚
resolution
range. The solution of the rotation function was used
to perform the translation function calculations in...
... and the human genome. It stands to reason
that it might play critical role in several human pathol-
ogies, in particular age-related disease.
DNA methylation enzymes
In mammalian cells, DNA methylation ... expression by DNA
methylation. Cytosine methylation at the DNA
sequence d(GGCGCC)2 triggers an extended eccentric
double-helix structure called E -DNA. Like B -DNA,
E -DNA...