... whether
the aspartic proteinase genes are also expressed in the guard
cells of leaves; if so, this could explain the greater expression
of the AtPaspA1 gene observed in the light.
The aspartic proteinases ... glycosylation; proteinase.
We have been studying the aspartic proteinase genes in
Arabidopsis with the goal of understanding their function in...
... considered to display differences in the fold of
the main chains. None of these includes any of the
binding sites, but some are located in the vicinity.
Three of the regions with different conformation
involve ... similar
arrangement, and the side chain of Ser97 in VPRK is
too short. One side of the loop is involved in the bind-
ing of the P2–P4 residues o...
... To
overcome the fact that the prosa z-score is dependant of the
length of the amino acid sequence, the z-score was
normalized using the natural logarithm of the sequence
length [39]. The resulting ... helix and the FG loop correspond to that region of
the PAS fold that is part of the region which tethers the PAS
Table 4. Backbone root mean square deviation v...
... changed either in the presence or
absence of the metal cofactor. By contrast, the sub-
strate binding affinity of the RNase H domain proba-
bly increases in the presence of the metal cofactor
compared ... alter the interaction between the sub-
strate and metal ion, such that the scissile phosphate
group of the substrate and the Mg
2+
ion are
arranged ideally. T...
... phenylalanine 108 supplied
from the other subunit exists in the proximity of the
x-end [24].
These results led to the hypothesis that the prenyl-
chain of the product elongates in the space enclosed by
a-helices ... in the center of the
cavity and likely to act as the bottom of it in these
structures; however, the structural information is inde-
cisive with respe...
... deter-
mining the kinetic features of PKH. However, the data
for the catalytic efficiency of ginkgotoxin and DPN are
of the same order of magnitude and reflect their antivita-
min character, as they are ... the antivitamin character of both compounds
and explains a depletion of cofactor formation in the
organism in the presence of the antivitamins.
The kineti...
... positions of the corresponding side chain, Arg220,
the NADPH cofactor and the substrate analog hexose
in the MUR1 structure (Fig. 11). Some of the distances
between these groups are rather long ... Stereoview of the RMD monomer. The cofactor-binding domain and the substrate-
binding domain are shown in aqua and light sand, respectively; the APPR portion of the...
... the relationship between the
efficiency of protein N-myristoylation and the identity
of the amino acids at positions 3, 6 and 7 of the con-
sensus motif in plant target proteins with the use of ... proteins of the expected size. The positions of pro-
tein size standards are shown on the left of the gel.
Table 1. Observed and calculated mass values for the...
... the expression of the rat,
mouse and human homologues of ERO1-Like protein a but
not of the isoform ERO1-Lb are stimulated by hypoxia in
rats vivo and in rat, mouse and human cell cultures. The
temporal ... speculate from these findings that
the expression of ERO1-La is probably regulated via the
HIF-pathway and thus belongs to the family of classic
oxygen regulated...
... to
determine to what extent they follow the rules accounting
foridentitiesinprokaryotesandeukaryotes[1,4,5].Inspite
of the universality of the genetic code, there are often
barriers to aminoacylation ... accepted 1.1 nmol of serine per A
260
unit of
tRNA. The following archaeal tRNAs were prepared by
in vitro transcription [20] of their synthetic genes, constructed
accord...