... outer
membrane proteins that are structurally related but highly
polymorphic. These proteins were originally identified as
colony -opacity- associated (Opa) proteins [1]. Opa proteins
appear to play a critical ... conformation. In this
study, we describe the isolation and structural analysis of
opacity proteins OpaJ129 and OpaB128 derived from
Neisseria meningitidis strai...
... amounts of sialylated Hex3 glycoforms were indicated.
b
Minor amounts of Hex2 and Hex3 glycoforms lacking acetyl groups or
containing a second glycyl group were indicated.
c
Very minor amounts of ... flow
rate of 1 mLÆmin
)1
.
Preparation of oligosaccharides
O-Deacylation of LPS with hydrazine. O-Deacylation of
LPS was achieved as previously described [ 21]. Brie y, LPS
(10 mg) w...
... al. (Eur. J. Biochem. 269) Ó FEBS 2002
Conformational analysis by CD and NMR spectroscopy of a peptide
encompassing the amphipathic domain of YopD from
Yersinia
Tobias Tengel
1
, Ingmar Sethson
1
and ... class of
Yersinia Yop proteins. Infect. Immun. 61, 71–80.
39. Slupsky, C.M., Kay, C.M., Reinach, F.C., Smillie, L.B. & Sykes,
B.D. (1995) Calcium-induced dimerization of tr...
... anti-
body-based Western array that can rapidly analyze
the expression levels of many proteins at once. We
selected 40 apoptosis-related proteins and determined
that 22 of those proteins were ...
various proteins is essential. Recent advances in mo-
lecular biology and biochemistry have enabled analy-
sis of the expression profiles of numerous proteins at
once.
In this...
... of hCDC4 can also be observed in
Acute Myeloid Leukemia (AML) or high risk
Myelodysplastic Syndrome (MDS). Therefore we
carried out a mutational analysis of the hCDC4 gene in
35 samples of ... Big Dye Terminator
system (Applied Biosystems, Germany). Sequences
were analyzed using the Chromas software (Technely-
sium Pty Ltd, Tewantin, Australia) and GeneDoc
software (http://www....
...
levels of many of these genes identified in the array
analysis. Moreover, Western blot analysis of thymo-
cyte lysates also confirmed that IgM increases with
age (Figure 3). Flow cytometry analysis ... and array analysis. For each array
sample, the RNA was prepared from the purified
thymocytes. The thymocytes were processed by using
the RNEasy Mini Kit (Qiagen, Valencia, CA...
... capacity of DM64 to inhibit the myotoxicity induced
by myotoxins I (Asp49) and II (Lys49) from B. asper venom
was analyzed in vivo and in vitro. DM64 effectively neut-
ralized the myotoxic and cytotoxic ... as marker.
Chemical deglycosylation
DM64 was chemically deglycosylated with anhydrous
trifluoromethanesulfonic acid (TFMS) using the GlycoFree
kit k-500 from Oxford GlycoSystems, USA...
... chaperoning in vitro [66], and disassembly of active units
from an oligomeric (storage) state of a-crystallin was
proposed, upon structural analysis of aA-crystallin by site-
directed spin labelling, ... example of stress resistance, fully hydrated cysts
survive several years at physiological temperature in the
complete absence o f o xygen [48,51,52,54], an unusual degree
of tol...
... reactivity of anti-tTG Igs. The
cloned human antibodies to tTG, expressed as scFv, were
previously selected from phage antibody libraries obtained
from intestinal biopsy lymphocytes from three ... lymphocytes of CD patients
[16]. We were able to isolate single-chain antibody
fragments (scFv) to tTG from all intestinal lymphocyte
libraries but not from those obtained from perip...
... N-acylated with acyl
2
. All acyl C-1 signals were
identified from H-2:C-1 HMBC correlations. C-1 of acyl
2
gave HMBC correlation to H-2 of GlcN B; C-1 of acyl
4
showed correlation to H-3 of acyl
2
. ... Acyl
1
,acyl
2
and acyl
3
residues had hydroxy or acyloxy groups at C-3 (
13
C signals
of C-3 at 69.0–72.4 p.p.m.), while acyl
4
had no substituents.
The signals of acyl chains c...