... tyrosin-
ase and promotes melanin synthesis in the ink gland of the
cuttlefish Sepia officinalis through the nitric oxide/cGMP signal
Ó FEBS 2003 Tyrosinase from the mollusk, Illex argentinus (Eur. ... Naraoka et al. (Eur. J. Biochem. 270) Ó FEBS 2003
Purification, characterization and molecular cloning of tyrosinase
from the cephalopod mollusk,
Illex...
... formation of the disulfide pairing [44] and is
also conserved throughout these sequences of all the
members of subfamily 1 of a-KTx, including PBTx3.
Because of these similarities and conservation of the
consensus ... we
present the purification, primary structure and functional
characterization of PBTx3, a novel peptide inhibitor from
the venom of the P....
... where the presence of McsA enhances the
autophosphorylation of McsB several-fold. We studied
the binding pattern and the thermodynamic aspects of
this interaction. The thermodynamics of the McsB–
McsA ... dehydra-
tion of the protein interfaces, provide thermodynamic
stability for the complex and drive the interaction
[33,34].
In order to estimate the kinetic...
... purified
from the gut of larvae of the yellow-spotted longicorn beetle
Psacothea hilaris by acetone precipitation and elution from
gels after native PAGE and SDS/PAGE with activity
staining. The purified ... (Amersham
Bioscience) was used for isolation of mRNA from P. hilaris
larval midguts. First-strand cDNA synthesis from the
isolated mRNA and the following ampli...
... to the stimulation of the cytosolic isoen-
zyme observed in spinach and other plants [27]. These
data suggest that the purified protein was the chloro-
plast isoenzyme, although the level of inhibition ... protein of 52 kDa (Fig. 1).
The 52 kDa protein was excised from a Coomassie-
stained gel and digested with trypsin, and the resulting
peptides were analyzed by ES...
... we
report the purification, enzymatic characterization and
analysis of the composition of the diol dehydratase of
L. collinoides.
MATERIALS AND METHODS
Bacteria and culture conditions
The lactic ... obtained from the previous
construction. The new construction (pQE30DD) contained
the sequence of the a, b and c subunits genes and their Shine
Dalgarno sequ...
... first introns and exons of
Y1, Y2andY3.
An alignment of the peptides predicted from the
pleurocidin genes and pseudogenes is shown in Fig. 1. All
of the genes, and even most of the pseudogenes, encode
highly ... promoters from WF1 and WFX relative to WF2
and WFYT, the sequences differed at only five locations
and four of these were in a stretch of eight pos...
... induced against the linear isoform of the HNE
peptide. Although the binding pattern may be somewhat
blurred by these antibodies and by the polyclonal nature of
the sera, the importance of residues ... truncated from the N- and
the C-termini and the shortened analogues were assessed for
binding of mAb BH216 (Fig. 4). The five first amino acids
of the N-ter...
... cloned from venom glands of
one of the snakes. Comparison of their mass and N-terminal sequence
revealed that all the PLAs were conserved in both KBf venoms, but that
two of their 3FTx isoforms ... the prey of Bf
and king cobra consists mainly of snakes and reptiles,
which are distinct from those of other kraits (e.g.
B. candidus and B. multicinctus, which prey...
... Apart from
cleavage of the signal peptide and the propeptide,
which happens in the maturation process for every
conotoxin [8], the removal of the two additional resi-
dues Leu and Val at the C-terminus ... elution
conditions, the retention times of SFWT and S
FWT
were clearly not the same as the retention time of
the C-terminal tetrapeptide fragment from...