... generate. The human
Tom70 receptor is an important example of such refractory
targets, and the generation of a high-affinity reagent specific
for the cytosolic domain is a potentially valuable tool in the
study ... antigen receptor antibody from sharks;
VNAR, single variable domain of the IgNAR antibody; Tom70,
human 70 kDa component of the transl...
... lL of a 1% erythrocyte
solution was added from the animal that showed the high-
est hemagglutination titer for each isoform, human
erythrocytes for P I and P II, and rabbit for P III and IV.
The ... indicate the M
r
values of the standards in thousands. Lanes 2–9, 5 lg of peak
P I, P II, P III ⁄ P IV and a mixture of other isoforms (*), respec-
tively, in th...
... demonstrated
for plants. Therefore the tentative annotation as an
ACC deaminase is probably not correct and the
deduced protein might be a good candidate for the
first d-CDes enzyme in higher plants of ... [56] using
BSA as a protein standard. The DNA and amino acid
sequence analyses and prediction of the molecular masses
were performed with the programs mapdraw an...
... combinations of degenerate and specific primers
and by rescreening the cDNA libraries. The partial clones
of XHIVEP2 and -3 covered 5.9 and 3.2 kb of the respective
3¢ ends and included 3¢-UTRs and poly(A)-tails.
A ... response,
and the HIVEP1 protein has been shown to activate
transcription of the human immunodeficiency virus
enhancer in human [46] and...
... Nakamura
2
and Yoshihiro Katayama
1
1
Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology, Koganei, Tokyo,
Japan;
2
Forestry and Forest Products ... separated into supernatant and residue by
centrifugation at 60 000 g for 60 min at 4 °C. The resultant
supernatant and residue were designated the cytoplasm
fraction (C...
... 689
Soret and Q (a and b) bands of the oxy form and the
CO-bound form do not show specific differences from
those of mammalian or bacterial HOs. The small band
at 634 nm seen in the spectrum of the ... exhibiting the bands of the remaining oxy
complex. The 340 nm-band of NADPH decreases in
proportion to the decrease of Soret band at 410 nm and
to the i...
... with financial support from
the Research Foundation of Raisiogroup (Raisio, Fin-
land), the Finnish Funding Agency for Technology and
Innovation (TEKES) and the Commission of the Euro-
pean Communities, ... assay.
Stereospecificity and substrate specificity
The stereospecificity of T. reesei TYR2 was studied by
following the activities on 15 mml-dopa, dl-dopa and
d-dop...
... showing the nuc-
leus, and Fig. 7C, F, K and N are the merged images
of A and B, D and E, I and J, and L and M, respect-
ively.
Discussion
Nature has developed many pathways for combating
and tolerating ... treatment of pea seedlings were cold (A and B), salinity (C and D),
wounding (E and F), drought (G and H), SA (I and J), ABA (K and L) and calcium (...
... with the sequences of zebrafish (z), chicken (c),
mouse (m) and human (h) HSF1. The three domain structures, the DBD and the hydrophobic heptad repeats (HR-A/B and HR-C), are boxed. Open
and filled ... zebrafish,
chicken, and human HSF1 (Fig. 3B); for instance, the
DBD of rainbow trout HSF1b shared 90.7% identity with
the DBD of human HSF1. By contrast, reg...
... of
Bip-[
3
H]Pro.
Results and Discussion
Synthesis, chemical characterization and stability
of Bip-Pro
Figure 1 shows the structure (Fig. 1A) and the synthe-
sis strategy (Fig. 1B) of Bip-Pro. The purity of the
compound ... probes are required for
further studies on the mechanism of transport func-
tion and the structure of the carrier proteins. For
exa...