... Role of
Acinetobacter calcoaceticus
3,4-dihydrocoumarin hydrolase
in oxidative stress defence against peroxoacids
Kohsuke Honda, Michihiko Kataoka, Eiji Sakuradani and Sakayu Shimizu
Division ... 3,4-dihydro-
coumarin hydrolase; perhydrolase; peroxoacid.
In a previous study, we found and isolated a novel
lactonohydrolase, 3,4-dihydrocoumarin h...
...
motif
Arg40
Cys84
Tyr349
Tyr347
Lys370
Ala36
Ala36
Ala36
Val357
Phe518
B
Laccase
Tyrosinase
β
-sheet protein
Fig. 1. Overview of the tyrosinase gene
and surrounding genes in the genome of
V. spinosum. (A) Showing the tyrosinase
gene and those in its immediate vicinity in
the ... domain structure of the V. spino-
sum tyrosinase in more detail, we determined protein
stability...
... shown that interactions between
KCs and endotoxin comprise the initiating event lead-
ing to hepatotoxicity in liver injury, including endotox-
emia and ischemia ⁄ reperfusion injury [14]. In our
studies, ... mediator of lethal sepsis, triggering the
production of proinflammatory cytokines and liver
injury [13].
Therefore, the present study aimed to elucidate the
role of KCs...
... have indi-
cated that the CagA
PY
-induced cell elongation pheno-
type also involves tyrosine dephosphorylation of
cortactin, vinculin and ezrin, which are three well-
known actin-binding proteins ... the amount of intracellu-
lar CagA
PY
. Cortactin, ezrin and vinculin are all Src
substrates, and Src inactivation causes dephosphoryla-
tion of these proteins and is crucial for t...
... CerK results
in decreased levels of intracellular Cer, and
the inhibition of stress- induced cell death.
The interaction between CerK and PPARb
may play an important role in regulating epi-
dermal ... survival resulting
from activation of PPARb.
Activation of PPARb by LD induces CERK mRNA
expression and increases CerK activity in SP1
cells
To investigate the role of...
... compartmentalization and cytotoxic action of cholera toxin in
rat liver parenchyma. Following administration of a saturating dose of
cholera toxin to rats, rapid endocytosis of both cholera toxin subunits ... prepared
from CT and CT-B-injected rats (Fig. 3A). An increase
in ARF content was observed in endosomal fractions
isolated 5 min postinjection of CT-B, and this inc...
... vector by using Lipofectamine (Invitrogen).
Cells were selected in neomycin, and clones were isolated
and screened for Bcl-X
L
expression.
Creation of TAM67-overexpressing cell line
The dominant-negative ... transcription, were increased in response to
EGF. Pretreatment of the cells with PD98059, an inhibitor of MAP kinase
kinase, inhibited the EGF-induced c-Fos and c-Jun expr...
... charged amino acid side
chains, R397 and R120, are also involved in fosfomycin
binding, and in view of the multitude of interactions, it was
assumed that deletion of a single interaction would not ... chain of K22 participates in
fosfomycin binding, thus providing a rationale for the loss of
fosfomycin binding to the K22V and K22E mutant proteins.
However, two other posi...
... on
conformation of inhibitor main chain. Thus, different
inhibitors differ not only in t he binding affinity, bu t a lso
in the degree o f freedom of the inhibitor inside the binding
tunnel. Some s pecial inhibitors ... that t he strain imposed on inhibitor during
docking into the binding site of pr otease does not allow
correct orientation of the hydroxyl group to catalytic
aspart...
... five amino acids of the
extracellular domain and devoid of any tag was not
functional [27], while a mutant containing four amino acids
of the ectodomain and including a rhodopsine tag was
found ... periods of times (0–60 min)
at 37 °C. The concentration of the biotinylated Ig remaining
on the cell surface was then quantified by measuring the
binding of [
125
I]Streptavidin. In...