... biochemical
analysis of the export apparatus. It is also a major problem
that, in most cases, only very limited information is
available about the motifs that target cargo proteins to
the nonclassical ... proteins in mammalian cells revealed the
existence of a machinery that is capable of translocating the
protein across the plasma membrane [103], demonstrati...
... interaction. (A) The sequential binding
of the ligands to each half of the receptor
dimer induces the recruitment of two
molecules of b-arrestin and then the
activation of ERK. (B) Only one molecule
of ... plasma membrane and to
stimulate ERK1 ⁄ 2 phosphorylation. All these data
indicate quite clearly that homo-oligomerization and
hetero-oligomerization have a pi...
... addition of
information to the model. This interaction of the
evaluation of a formula and the expansion of the
model produces several linguistically interesting
side-effects,
and
these
have ... evaluates a formula with
respect to a model has been augmented with a table
that contains each ~-expression and the ima6e of
its denotation in the current...
... including
anaesthetic and surgical procedures, as well as animal man-
agement, have been reviewed and approved, and were
performed in accordance with the relevant China national
legislations and regulations.
Recombinant ... blot analysis showed that the effi-
ciency of PrP
Sc
propagation was enhanced by the
addition of NADPH, which was closely related to
increasing NADPH conc...
... binding at
the active site of RNase A. The r.m.s.d. between the
RNase–AMP complex and the unliganded protein are
0.43, 0.44 and 0.59 A
˚
for the Ca, main chain and side
chain atoms of 123 equivalent ... R
free
value. The second AMP conformation
has the phosphoryl group away from the P
1
subsite
and as it is a minor conformation it was not included
in the s...
... primers
(forward, 5¢-TAATACGACTCACTATAGGTACTATG
TATCGCATGCCAAT-3¢; reverse, 5¢-TAATACGACTC
ACTATAGGTACTTTAAAGTCCCGGGTTGA-3¢). For
PCR, the reaction mix consisted of 1 · Taq buffer contain-
ing 1.5 ... the
tiger prawn Penaeus monodon. As there is a high degree
of sequence similarity and structural conservation of
these genes and gene products, confirmation of the true
identi...
... probe the functional effects of dimeriza-
tion ⁄ oligomerization on mechanisms of ligand binding
and extent of activation (i.e. pharmacological efficacy),
as well as on the involvement of protein protein ... information can be used to predict pro-
bable regions involved in protein protein interactions.
Many of the limitations and considerations of accuracy
and r...
... Plecoptera, Dermaptera, Orthop-
tera, Phasmatodea, Mantodea, Isoptera and Blattaria,
but not in Protura, Diplura, Ephemeroptera, Odonata
and the Eumetabola (Holometabola + Hemiptera)
(Fig. 3). In addition, ... Hexapoda and provides strong evidence
for the monophyly of the Polyneoptera (Plecoptera, Dermaptera, Orthop-
tera, Phasmatodea, Mantodea, Isoptera, Blattaria) and the...
... (DTT), aggregation
and precipitation of a- lactalbumin commenced after
25 min and reached a plateau after 90 min. The
amount of DTT-induced aggregation of a- lactalbumin
was increased in a concentration-dependent ... ordered and disordered
protein aggregation
Heath Ecroyd and John A. Carver
School of Chemistry and Physics, The University of Adelaide, Australia...
... digestion of the DNA to mononucleosome size of samples con-
sisting of a mixture of hepta-, octa- and nonanucleosomes and half the amount of hexa- and decanucleosomes (6- to 10-mer sample). (A) (a)
Nonamer ... mononucleosomes and
the band S. DNA gel electrophoresis of the fractions
from Fig. 3D,E showed that approximately 90% of
the DNA in the main peak as...