Báo cáo khoa học: The chloroplast ClpP complex in Chlamydomonas reinhardtii contains an unusual high molecular mass subunit with a large apical domain doc
... chloroplast ClpP complex in Chlamydomonas
reinhardtii contains an unusual high molecular mass
subunit with a large apical domain
Wojciech Majeran
1
, Giulia Friso
2
, Klaas Jan van Wijk
2
and Olivier Vallon
1
1 ... Cyanobacteria
[22] and in the genome of the Cyanophora cyanelle, an
ancestral chloroplast. In the green alga Chlamydomonas
reinh...
... isolated TnC are
well known. Sites III and IV in the C -domain (carboxy
terminal) bind Ca
2+
with higher affinity, while sites I and II
in the N -domain (amino terminal) bind Ca
2+
with lower
affinity ... are sensitive to Ca
2+
binding to the
two classes of sites, and the authors interpreted the high
affinity sites being in the C -domain and the low affinity in
the...
... similar abundances and
can be assessed quantitatively) in the dinucleosome
sample is indistinguishable from that of high- molecular
weight chromatin. Furthermore, in the DNase I foot-
print of the ... that approximately 90% of
the DNA in the main peak as well as the mononucleo-
somes and band S are all in the 140–160 bp size
interval (not shown). The high percenta...
... CYLD in air-
way epithelial A5 49 and NHBE cells in vitro and in vivo.
As shown in Fig. 5A, B, NTHi up-regulated CYLD in
A5 49 and NHBE cells as well as in the lungs of mice
intratracheally inoculated ... 4 signaling pathway acts as a
positive regulator for TLR2 induction by bacteria via a
dual mechanism involving functional cooperation with
NF-kappaB and MAPK phosphatas...
... osmoregulation and sporulation. Isola-
tion and characterization of yeast mutants blocked at various sta-
ges of transport pathways are an invaluable method for
unravelling the molecular details of vacuolar ... binding of apoE. (iii) ABCA1,
syntaxin-13 and flotillin-1 operate in both loading conditions
with different response rates and downstream signalling involving
an inward rect...
... formulate an operational defini-
tion of 'affix'.
The words in Class
II, typified by REPLACE, have the
property that the internal-consonant string is an ad-
missible initial-consonant ... examine the Class
IV
words.
The Class
IV words are distinguished by the property
that the internal consonant string is neither an admis-
sible initial- nor an admis...
... choice rather than by search ~lsing general
inference.
The paper also indicates what additional requirements
are needed for a full treatment of pronominal anphora. These
include use of a representation ... research done at the Artificial
Intelligence Laboratory of the Massachusetts Institute of
Technology.
Support for the laboratory's artificial intelligence
research i...
... of the authors of the pa-
per and another human annotator (not a project
member) corrected the automatic annotations. The
annotators had a reference manual and trained
on trial sessions of the ... dialogues. To test inter-
annotator reliability, the author and external anno-
tator labeled the same 757 examples taken from
non-training data; the resulting inter-annotator re-...
... ananatis, as the template, the crtE gene
encoding GGPS was amplified using PCR with KOD DNA
polymerase (Toyobo, Osaka, Japan) and the primers: PaG
GPS-Fw, 5¢-AAGAAA
CATATGACGGTCTGCGCAAA
AAAACACG-3¢, ... such as
geranyl diphosphate synthase (GPS) and longer-chain
prenyl diphosphate synthases.
Results
Refolding and purification of recombinant
P. ananatis GGPS
P. ananatis GGPS and the mu...