... CYP17 5A1 is used as an inter-
mediate for the synthesis of thermozeaxanthins and
thermobiszeaxanthins, which are the main carotenoids
of T. thermophilus [15]. The insertion of thermozeax-
anthins and ... TSIPGVYACGDIVTYPGKLPLIVLGFGEAAIAANHAAAYAN-PALKVNPGHSSEKAAPGT 335
E.
coli 277 TSIPGVFAAGDVMDHI YRQAITSAGTGCMAALDAERYLD GLADAK 321
A.
pernix 283 TSIPGIFAAGDCTSMWPGFRQVVTAAAMG...
... (sense) and
5¢-GAAAAAACGCGATCCTACTT-3¢ (antisense). Primers
for unmethylated DNA were: 5¢-GAAGTAGGTGGAGT
ATTGAAT-3¢ (sense) and 5¢-CAAAAAAACACAATCCT
ACTT-3¢ (antisense).
Caspase 3 activity
Cells ... Relative
fluorescence intensity was determined using imagemaster
2d elite software 4.01 (Amersham Bioscience, Uppsala,
Sweden).
Statistical analysis
Data in bar graphs are expressed as the...
... evolutionary aspects of invertebrate tachykinin
and tachykinin-related peptide
Atsuhiro Kanda, Kyoko Takuwa-Kuroda, Masato Aoyama and Honoo Satake
Suntory Institute for Bioorganic Research, Osaka, Japan
Tachykinins ... Kanda A, Iwakoshi-Ukena E, Takuwa-Kuroda K &
Minakata H (2003) Isolation and characterization of
novel tachykinins from the posterior salivary gland of
the com...
... selenium-
containing GPX mimics. We also studied the catalytic
mechanism using steady-state kinetics of 6-CySeCD
catalysis and investigated the antioxidant ability of
6-CySeCD using a mitochondria injury ... NADPH
were also obtained from Sigma. Sephadex G-25 was pur-
chased from Pharmacia (Uppsala, Sweden). All the other
materials were of analytical grade and obtained from
Beiji...
... Thomas-Oates, J.E. & Brade, H. (1994) Preparation
and structural analysis of oligosaccharide monophosphates
obtained from the lipopolysaccharide of recombinant strains of
Salmonella minnesota ... which links the O-7 of a Hep residue, and a
2-amino-2-deoxy galactose (GalN), which is the branching
point of the oligosaccharide. The amino function of the GalN
res...
... significant levels of identity to
sequences of 2-aminomuconate deaminases [6,8,27] or to
any other sequences available in FASTA and BLAST
database programs at the DNA Data Bank of Jap an.
Recently, ... the
determination of released ammonia. The coupled enzyme
assay revealed the mechanism of the deamination reaction
and the subsequent metabolism, including the deaminat...
... to clone the
MABO gene. The DNA fragment carrying the MABO
ORF was amplified with the primer pair 5¢-GAC
CTGAGTAGAAATGGATCCCTGA TGGACAGG-3¢
and 5¢-GGAATGGCTCGAGGGATCATCACC-3¢ bear-
ing the restriction ... role in the
biodegradation of a n a lmost unlimited spectrum of natural
and man-made organic compounds, among them the
tobacco alkaloid nicotine. Perhaps analysed in...
... least at
current stage. In this paper, we study a feature-based
approach that basically integrates entity related
information with context information.
3.1 Classification Features
The classification ... classifiers and the
remaining to evaluate results.
The aim of the first set of experiments is to examine
the role of structure features. In these experiments, a...
... 17 base pairs. The
schematic structure of each substrate is shown on the left side of the
autoradiogram of the gel. The percentage unwinding is shown on the
topofeachpanel.Ineachpanel,lane1isthereactionwithout
enzyme, ... VI)
showed a linear rate up to 30 min (Fig. 4A) . After further
incubation it deviated from the linearity and became
saturated at 60 min. Titrati...