... FEBS
MD simulations of the RBP molten globule state E. Paci et al.
Characterization of the molten globule state of
retinol-binding protein using a molecular dynamics
simulation approach
Emanuele Paci
1
, ... unfolded states of human
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31 Marchi M & Ballone P (1999) Ad...
... Foun-
dation. SK was supported by the Maj and Tor Nes-
sling Foundation and PR was an Academy Research
Fellow of the Academy of Finland.
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Enzyme purification and assays
To pur...
... lower
than that of isomaltase, respectively. The K
m
for a- pNPG of
Mun/Bpu was the same a s that of isomaltase, whereas the
K
m
for a- pNPG of M un/Bst was about 50 times l ower than
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Saccharomyces cerevisiae
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what information is available about the AS/400 system.
Candidates AJ N V AJ AJ DET N N
for the POS AV AV
of each word PN PP
Phrases what information is available about the
appears in sentences ... parser, and a
matching pattern with a part of speech rather than
an actual word on one side can be regarded as a
relaxation rule, in the sense that syntactic and se-
manti...
... (5¢-GCATAGCGATGTGGACGA-3¢) and
QaCgClp2 (5¢-GAGGACCGAGACCGTGAA-3¢). The
abbreviations ‘Qs’ and ‘Qa’ refer, respectively, to sense and
antisense primers. Accurate amplification of the target
amplicon was checked by obtaining ... sequencing
chemistry.
Real-time quantitative PCR
Quantitative RT-PCR analysis was performed using the
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... 5¢-ATA
CCATGGACAAAACCCACAGTACAATG
P1OH 5¢-
CATATGCACAAAACCCACAGTAC
P2O 5¢-TAT
GGATCCTTAATTAGAAACAAACTGTCTGA
TAAAC
P2OH 5¢-
CTCGAGATTAGAAACAAACTGTCTGATAAACC
P1Z 5¢-ATA
CCATGGCTGGAGAAGACTTTAAGATC
P1ZH ... min, the absorbance of
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2–100 l
M
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tate lyase, releasing unsaturated trigalacturonate as the
major product [20]. We here report on the overproduc-
tion, purification an...