... Journal 272 (2005) 1291–1304 ª 2005 FEBS
Isolation and characterization of a D-cysteine
desulfhydrase protein from Arabidopsis thaliana
Anja Riemenschneider, Rosalina Wegele, Ahlert Schmidt and ... value, and
safety of d-amino acids. Adv Exp Med Biol 289, 447–
481.
39 Lohmann KN, Gan S, John MC & Amasino RM
(1994) Molecular analysis of natural leaf senescence...
... Mare
Â
chal, P.,
Miginiac-Maslow, M. & Meyer, Y. (1994) Arabidopsis thaliana
NADPH thioredoxin reductase: cDNA characterization and
expression of the r e combinant protein in Escherichia ... Storz, G. &
Rhee, S.G. (1994) Cloning and sequencing of thiol-speci®c
antioxidant from m ammalian brain: alkyl reductases and thiol-
speci®c antioxidant de®ne a large family...
... within
the last 30 years. The greatest number of RIPs have
been found in the Caryophyllaceae, Sambucaceae,
Cucurbitaceae, Euphorbiaceae, Phytolaccaceae and
Poaceae [1]. Although many are potentially ... Roberts
3
and Ana Paula U. Arau
´
jo
1,2
1 Programa de Po
´
s-graduac a o em Gene
´
tica e Evoluc a o, Universidade Federal de Sa˜o Carlos, Brazil
2 Instituto de Fı
´
sica de Sa˜o Carlos...
... (Forward: 5¢-ACAAGGG
TAGACCAAACACCAAGAACAGCAACAAAAGAG
ACGGGCGAATCACTGACCATCAACgccGTCCTGA
GAGAT-3¢) and N8518 (Reverse: 5¢-TTTCACGGTTAA
TGCGGTGCCAGCTCCCCAACTGTAATAAATACC
AGACAAATTATATGCTCCaacCCTATACGTGCCA
CTG-3¢); ... of two protein chains, each containing
one variable and five constant domains, and functions as an
antibody. In order to assess the antigen-binding capabilities
of iso...
... temperature (1 h). Sequences
of the upper strand of the duplexes are listed below.
Sites of mutation are underlined: wt, 5¢-AGAGAGAA
TGAGAGGCTTCCCAATAGC-3¢;mut1,5¢-AGAGAG
AATGA
TAGGCTTCACAATAGC-3¢;mut2,5¢-AGAG
AGAATGA
TAGGCTTCCCAATAGC-3¢;mut3,5¢-AGA
GAGAATGAGAGGCTTC
ACAATAGC-3¢.
Binding ... XHIVEP1,
5¢-ATCCAGAGGCAGAAGCAG-3¢ and 5¢-CTGCATT
CAGAGTAAGCC-3¢,60°C, 29 cycles; XHIVEP2,
5¢-AA...
... fungal
tyrosinases, from both a structural and a functional
point of view, are from Agaricus bisporus [10] and
N. crassa [1]. Also, a few bacterial tyrosinases have
been reported, of which Streptomyces tyrosinases ... tyro-
sinases from N. crassa [26–28] and Agaricus [28] and
Pycnoporus species [25] have an additional C-terminal
domain that is proteolytically released...
... inves-
tigation because of their physiological importance and
their pharmaceutical relevance as drug carriers [1–6].
Both transporters catalyse the uptake of most dipep-
tides and tripeptides and a variety ... was inhibited not only by
unlabeled Bip-Pro itself, but also by well known sub-
strates of H
+
⁄ peptide cotransporters, such as Gly-Sar,
Ala-Ala, Lys-Lys, Ala-Asp, d-Phe-...
... act as an NADH oxidase in vivo, instead act-
ing as a CoADR. This is only the second demonstra-
ted CoA reductase activity, and the first appearance of
this activity in both the Archaea and in a ... concentration
(as determined at 460 nm). blast and tfasta analysis
of the phCoADR revealed a significant level of identity
to putative NADH oxidases from hyperthermophiles
and...
... (5¢-GCGCG
GGATCCTCATTTAAGCAT
GAAAACAACTTTGCC, antisense), containing NcoI and
BamHI sites (underlined in the sequences). In order to
introduce an NcoI restriction site, an extra alanine codon
(GCA) was ... A short-
chain AdhA and an iron-containing AdhB encoded by
the lamA operon [10], and an oxygen-sensitive, iron
and zinc-containing alcohol dehydrogenase which has
been purified fr...