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struction and characterization of mutations at
codon 751 of the Escherichia coli gyrB gene that confer
resistance to the antimicrobial peptide microcin B1...
... interacts with N10 and E97 of the second
subunit. The backbone NH group of R98 forms a
hydrogen bond with the backbone CO of F102. Fur-
thermore, the orientation of the side chain of the two
residues ... Sweden)
eluted with a linear gradient of 0–1 m NaCl. The fractions
containing the protein were pooled and precipitated by
addition of ammonium sulfate to a concentration of...
... R, Thomson
CT, Sacchettini JC & DePinho RA (1994) Evolutionary
relationships and functional conservation among verte-
brate Max-associated proteins: the zebra fish homolog
of Mxi1. Oncogene ... isoforms at the levels of Myc antagonism in
the REF assay, subcellular localization, and transcrip-
tional activity. Some of these analyses have assigned
differential functions to the two...
... Rochat
H, Harvey AL, Montenay-Garestier T & Menez A
(1990) Delineation of the functional site of a snake
venom cardiotoxin: preparation, structure, and function
of monoacetylated derivatives. ... the
membrane of cells.
Inhibitory effects of VVA1 on the hemolytic and
cytotoxic activity of VVA2
The effects of VVA1 on the hemolytic activity of
VVA2 were studied...
... containing
different concentrations (20–60 l
M
)ofproline.
Cloning of EF-Ts and construction of the EF-Ts mutant
Wild-type E. coli EF-Ts was cloned into expression plasmid
pAB146 (constructed by A. Bjo
¨
rnsson, ... at
the interception point between the exponential and the
Ôstarvation phaseÕ of growth, is 78% of the point of growth
inhibition of UY211 (D
420
¼ 0.60). The point...
... and
COX-2 expression (Figs 1 and 2 ). In ad dition, the ability of
RU486 to antagonize the repressive effects of 0.1 l
M
dexamethasone or budesonide was examined. In each case,
a concentration-dependent ... glucose, amino and fatty
acid mobilization, and loss of bone [2]. In addition,
endogenous glucocorticoids p articipate in feedback inhibi-
tion of the hypothalamo-pituita...
... high con-
centrations and become susceptible to modulation of
both function and expression. This, in turn, could
influence bioavailability, distribution and transport of
various dietary toxins and ... the
following conditions: reverse transcription (20 min at
61 °C), one cycle of denaturation at 95 °C for 30 s, and
PCR of 45 cycles with denaturation (15 s at 95 °C),
anneali...
... (1995)
Studies on the structure and function of glycosylated
and nonglycosylated neu differentiation factors.
Similarities and differences of the alpha and beta
isoforms. J Biol Chem 270, 4784–4791.
55 ... Neuregulin isoforms in dorsal
root ganglion neurons: effects of the cytoplasmic
domain on localization and membrane shedding of
Nrg-1 type I. J Neurosci Res 84,...
... the loss of b-sheet and the onset of
a-helix structure formation. To visualize the trend in
the formation of b-sheet as a function of increasing
SDS concentrations, the ratio between the bands at
1618 ... indicated time and analyzed
under native conditions. Oligomer distribu-
tion of 100 l
M proinsulin C-peptide in the
presence of divalent Ca
2+
and Mg
2+
ions
under nati...
... U2AF
65
interaction upon
phosphorylation of a conserved SF1 serine (Ser20)
within its U2AF
65
interaction domain [21].
Comparison of the structure of an RNA recognition
motif (RRM)-containing domain of the ... concentra-
tions of mock-phosphorylated SF1f or phosphorylated
SF1f with a constant concentration of U2AF
65
(1.2 lm) confirmed the enhancement of ternary
complex formati...