... further definition of the ligand recognition
pocket on the extracellular side of the receptors. This
has become a major obstacle to the further understanding
of the molecular mechanism of the ... conformation
change of the receptor and triggering the coupling of the
receptor with the G protein in the intracellular domain s.
The first step of bindi...
... (B) structure alignment of
the fragment 4–7 of the 13 ensemble structures of AII to the fragment 3–6
of the X-ray structure of AII.
Fig. 6. Structure of a representative folded conformer of AII ... Lennard-
Jones potentials are used. From the family of the 48
structures, 13 structures were selected having the best
allowed regions in the Ramachandran plot...
... [26]. These findings
lead to the hypothesis that Fe-bound ApeSOD mimics
the product-inhibited form and the shift of Tyr39 sup-
presses the release of the peroxide product. This may
be one of the ... the ter-
tiary structure of ApeSOD has not been elucidated.
In the present study, for the first time, we describe the
crystal structure of ApeSOD. In particular, we...
... Lys224–
Tyr228 (the back of the pocket) and the disulfide bridge
Cys191–Cys220 (the front of the pocket) (Fig. 4A). The
backbones of these segments form a deep hydrophobic
pocket with the negatively ... according to the residue in the mid-
point of the respective loop, as shown in Fig. 2. To
the east of the active site the 37- and 60-loops border
the S2¢...
... corresponding to the
N-terminus of the a2- helix and to the preceding loop
results in a rmsd of 1.0 A
˚
for the Ca atoms. Thus,
the movement of the a2 helix accounts for 23% of the
rmsd value (i.e. for ... determines the shape of the
active site entry. (e) The structure of the active site is
essentially identical with the active sites of the MshB
a...
... one-turn helix on the other face. It is thought
that the two inner strands of the b-sheet lie within the major
groove of the DNA and that a hydrophobic pocket formed
by the side chains of Y123 and ... [18]. The structure of the helical
coil a
2
/a
3
allows us to interpret the consequences of the
six mutations. As P153(152) and G162(161) are buried in
the pr...
... regrowth
upon the optic nerve crush, and also expressed in the
germinal neuroepithelium of retina, which generates
new neurons throughout the lifespan of the fish [11].
The cloning of the RICH proteins ... structures. The lowest-energy structure from the
RICH NMR ensemble is used for the overlay. (D) The surface of the RICH catalytic domain shows several negat...
... towards the
interior of the protein. The space that in the wild-type
molecule is occupied by the large hydrophobic side chain of
Fig. 3. Structure ensemble of HPr(I14A). The average structure of the
10 ... was
computed as the ratio of the s tandard deviations of the
chemical shifts of the amide nitrogen and p roton nuclei.
Results
Determination of th...
... part, and (b) the variability of the angle of
approach of the inhibitor relative to the catalytic
cleft of the enzyme. The latter factor may reflect not
so much the geometry of the catalytic site ... PW),
despite the lack of overall sequence similarity. The
role of the proline residue appears to be to maintain
the specific shape of the loop. The aroma...
... relative
orientations of the a and b domains. In one struc-
ture, the catalytic cysteines face each other; in the
other, the catalytic residues of the a domain face
away from the a¢ domain. The crystal structures ... mm of
monomers at 30 °C. Most of the
1
H-
15
N HSQC signals
of the dimeric form of PDI-bb¢ coincide with the signals
of the monomeric form or...