Báo cáo khoa học: Effect of valine 106 on structure–function relation of cytosolic human thymidine kinase Kinetic properties and oligomerization pattern of nine substitution mutants of V106 ppt
... Effect of valine 106 on structure–function relation of cytosolic human
thymidine kinase
Kinetic properties and oligomerization pattern of nine substitution mutants of V106
Hanne Frederiksen*†, ... nine
mutations: V106A, V106G, V106H, V106I, V106K, V106L,
V106M, V106Q and V106T. We then characterized the
enzymatic properties of the mutant e...
... linezolid and glycopeptides only, and Stenotropho-
monas maltophilia, sensitive to ofloxacin only. Polymicrobial
wound contamination also included contamination with fungi
(for instance, Candida ... Near-drowning
involved the aspiration of immersion fluids, marine and soil
debris into the respiratory tract and all patients displayed signs
of pneumonitis and pneumonia upon arriva...
... attentional failures after they had been working for
more than 16 hours on the traditional schedule [2].
Letter
Effect of intern’s consecutive work hours on safety, medical
education and professionalism
Christopher ... hours or more consecutive work
are unsafe. Efforts to reduce work hours should focus first
and foremost not on the frequency of extended-duration work
shifts, b...
... Characterization of siRNA–TRBP complex
formation after TRBP knockdown.
Fig. S4. Additional characterization of Dicer and
TRBP complexes.
Fig. S5. Effect of a terminal mismatch at the guide
strand 5¢ end on ... impact of silencing
TRBP and Dicer on the function of the fully paired
and mismatched sequences, we wished to examine
Fig. 1. Effect of guide strand 5¢ end mis...
... whether the
P-loop moves upon oxidation of the catalytic cysteine is
the ability of the conserved active site arginine to move
Effect of ionic strength and oxidation on PhyAsr R. J. Gruninger ... absence
of ligand, the WPD loop of PTP1B and RPTPa adopts
an open (inactive) conformation, and upon ligand bind-
ing, it adopts a closed (active) conformation (Fig. 3A).
In oxidiz...
... role of this region in the transcription of the
Ig-b gene. Deletion of region III decreased the expres-
sion of Ig-b mRNA to 40% of the expression in prede-
letion cells (Fig. 4A). Region III ... hybridization. Position of the probes, the size and position of restriction fragments hybridizable with the probe, and exon ⁄ introns are
shown as in Fig. 2A. Positions of the DT4...
... several cytoplasmic ligands. It is activa-
ted by millimolar concentrations of ATP and micromo-
lar Ca
2+
, and inhibited by higher concentrations
(> 100 lm)ofCa
2+
and by millimolar Mg
2+
. ... pore [10]. Results about the effect of the
luminal (trans) calcium on the activity of the RyR1 are
controversial depending on experimental conditions.
Inhibition of the channel...
... hydrolysis
of the substrates harboring these mutations was more
pronounced for the wild-type enzyme and nonactive-site
mutants (L90M and M46L) than for the active-site mutants.
Consequently, mutation of ... constant showed a strong
correlation with the volume of the P1¢ residue (correlation
coefficient R ¼ 0.90) and the number of hydrophobic
contacts the side chain formed wit...
... term
correspondences are estimated, in terms
of both computational complexity and
the performance of precise estimation of
bilingual term correspondences.
1 Introduction
Translation knowledge acquisition ... situ-
ation. One limitation of the corpus-based trans-
lation knowledge acquisition approach is that the
techniques of translation knowledge acquisition
heavily rely on avai...
... excitation and 520 nm emission filters. The
wells were loaded with solutions of a fixed concentration of
preformed quadruplex (50 nm) and increasing concentra-
tions of complementary strand (0–1 ... is composed of quartets
and interspersed loops, in the genome it is also flanked on each side by
numerous bases. The effect of loop length and composition on quadruplex
confor...