... microdomains (GEM) of the plasma membrane
and to be involved in the regulation of epidermal growth
factor receptor (EGFr or ErbB1) activity. To gain further
insight into the mechanisms involved in ... masses) of recombinant proteins are indicated.
(B) Protein profile of the gradient, visualized by Ponceau S staining of
the nitrocellulose membrane.
Ó FEBS 2004 EGF recep...
... Hung, L.M. & Lin, C.C. (1997) cGMP-elevating agents
suppress proliferation of vascular smooth muscle cells by inhibit-
ing the activation of epidermal growth factor signaling pathway.
Circulation ... Spain
Nitric oxide (NO
•
) strongly inhibits the proliferation of
human A431 tumour cells. It also inhibits tyrosine phos-
phorylation of a 170-kDa band corresponding to the...
... diglucosamine ring vibrational
bands at 1170 and 1045 cm
)1
, allowing calculation of
the inclination angle of the diglucosamine ring plane
with respect to the membrane plane [14]. In Table 1,
the ... liposomes corresponding to the com-
position of the macrophage membrane, phosphatidyl-
choline, phosphatidylserine, phosphatidylethanolamine and
sphingomyelin in a molar ratio of...
... showing that P2X
2–4
receptor proteins were induced by nerve growth factor
in these same cells [45,46]. In an additional cellular
model system for PD, consisting of SN4741 inducible
dopaminergic ... likely
depending on the intensity of the microglia reaction,
the kind of stimuli received and other local factors,
including cross-talk with neighbouring neuronal cells,
or induc...
... onformational changes
involving protein loops or backbone. T hus, the role of OBP
in signal triggering remains unresolved.
3
Keywords: crystal str ucture; domain swapping; odorant-
binding protein; olfaction.
Odorant-binding ... func-
tional human retinol-bin ding protein i n Escherichia coli using a
secretion v ector. Biochem. J. 296, 209–215.
41. Monaco, H.L. ( 2000) The transthyretin–r...
... local-
ization of iNOS to apical domains of intestinal epithe-
lial cells.
Particulate fraction association of iNOS in vivo
In order to determine the distribution of iNOS in
intestinal epithelial cells in ... enrichment in iNOS activity
within the particulate fraction of epithelial cells.
Apical distribution of iNOS in intestinal epithelial
cells
To get mor...
... extracellular matrix;
laminin binding protein; topological distribution; integral
membrane protein.
One of the primary events in the initiation of a disease is
thought to be the attachment of the causative ... anti-(laminin IgG), goat anti-(rabbit IgG), BCIP and NBT.
Lane 3, transferred proteins were incubated with BSA instead of
laminin.
Fig. 3. Radiolabeled laminin binding to (...
... tropomyosin
and actin, the IANBD probe retained its typical
responses to changes in Ca
2+
and S1 binding (Fig. 6).
Fitting binding curves to obtain binding parameters
is difficult in the case of Ca
2+
because ... troponin-tropomyosin:
inhibition of actomyosin ATPase activity without
inhibition of myosin binding to actin. J Biol Chem
256, 575–578.
36 Tobacman LS & Adelstein RS (19...
... FEBS 235
A
B
C
D
0 min 2 min 10 min 30 min
0 min 2 min 10 min 30 min
0 min 2 min 10 min 30 min
0 min 2 min 5 min 30 min
0 min 10 min 20 min 30 min
0 min 2 min 10 min 30 min
0 min
PKC-α
PKC-δ
PKC-α
PKC-δ
PKC-α
PKC-δ
PKC-α
PKC-δ
10 ... pattern of substitution
can contribute to the orientation, either sn-1orsn-2,
of the insertion of the DAG lactone into the binding
cleft of the C1 domain...
... the
untreated pellet after correcting for adhering medium, which
was determined from the amount of associated [
14
C]inulin.
Measurement of protein content
This was by a bicinchoninic acid assay kit (Sigma).
Statistical ... must rely on
an internal, luminal pool of CoASH.
Transport of
L
-carnitine into sealed microsomal vesicles
Transport of
L
-[
3
H]carnitine was studied by measure...