... against the logarithm of the relative
molecular mass (M
r
) of the standard proteins (s), or of the M
r
cal-
culated from the sequence for a dimeric *MtCM (d). Inset: appar-
ent oligomeric state of ... transfer of a nonmembrane protein
from the cytoplasm must occur directly into the sur-
rounding medium rather than into the periplasmic space,
the target compart...
... of the first 88
amino acids of PPI1 may participate in the interaction
with the H
+
-ATPase and makes PPI1
588
His
6
a suitable
tool to study the mechanism of action of PPI1.
The analysis of the ... 14-3-3s.
Results
The C-terminus of isoform 1 of the PM H
+
-ATPase
of A. thaliana (AHA1) interacts with the first 88 amino
acids of PPI1 [22], indicating that th...
... min, the absorbance of
the supernatant was read at 363 nm. The absorbance of
the reduced form of APAD (APADH) was linear over the
2–100 l
M
range of glutamate with a molar extinction
coefficient of ... complement the defect. The
relationship of all of these genes remains to be clarified.
Expression and purification of Sno1p and Snz1p
In light of the similarity i...
... CCAAT box at 324 bp upstream of
the major transcription start site. The presence of a CCAAT
box and the absence of a TATA box seem to be one of the
characteristics of the mouse integrin a3 gene.
We ... We
previously reported the structures of the mouse a3integrin
subunit gene including the exon/intron organization and the
alternative exon usage for the generati...
... products 1 and
3 from II, and products 3 and 4 from III. The cleavage of (b) leads
to product 4 from II, and product 6 from III. The cleavage of the
C5–C6 double bond (c) leads to product 5 from II, ... that the cleavage of the
C9–C10 double bond occurs independently of the
chain length of monocyclic apocarotenals, pointing to
the ionone ring as a determinant...
... oligomer
of the small form. The different influences of DPI on
the two forms could be caused by its failure to access
the flavin cofactor in the large form of complex I,
whereas the cofactor remains ... higher up in the gel
(Fig. 1B, lane 6). A subsequent incubation of the gel
in DPI resulted in inhibition of the lower band only
(Fig. 1B, lane 8). On the basis of...
... to all of the tRNA binding sites on the ribosome
(i.e. A, P and E sites) [13,14]. As expected, the addi-
tion of SF to the system resulted in pppGpp synthesis
(Fig. 2). The speed of synthesis, ... template,
the activity of the enzyme is similar to that in the
absence of tRNA. This is not surprising because the
template is needed to position tRNAs on the ribosome...
... structure. The confor-
mations of residues 8–9 are ill defined in the peptides. Thus,
the introduction of a prolinoamino acid in the C-terminal
tail of SP does not affect the overall structure of the ... log(DR–1)–log[B].DRisthedose-ratio[A]¢/[A] where [A]¢ is the concentration of agonist A, in the presence
of the antagonist B, which is equiactive to [A], the co...
... starting from Leu1. Therefore, the
requirement of Val at the N-terminus might be depen-
dent on the conformation of each protease. It would
be interesting to test whether or not the substitution of
the ... postulated in the experiment of
Fig. 2.
Mutation of the essential amino acid Ser237
Establishment of the E. coli expression system of
GluV8 enabled the rol...
... quality of
the end product (softer texture of ice cream, faster
ripening of cheese, etc.) and also lessens the problem
of lactose intolerance, which is prevalent in more than
half of the world’s ... ions (Fig. 2A). This
is higher than the theoretical value calculated from
the amino acid sequence of the mature protein
(109 301 Da). The asymmetric peak of the sin...