... Membrane transport of fatty acylcarnitine and free
L
-carnitine
by rat liver microsomes
Jason M. Gooding, Majid Shayeghi and E. David Saggerson
Department of Biochemistry and Molecular ... trans-ER membrane transport of palmitoylcarni-
tine together with an ER transport process for CoASH,
the cosubstrate for microsomal CAT [12]. In support of
the notion...
... N-terminal
sequencing.) At 8 h of incubation (lane 2), the bands
at 19 and 20 kDa had disappeared, and two clear
bands appeared at 15.0 and 13.3 kDa. These bands
are thought to be fragments of hTNF-a.At20hof
incubation ... After 8 h of incu-
bation, the bands at 19 and 29 kDa had disappeared, and two clear
bands appeared at 15.0 and 13.3 kDa. These bands are thought to
be fr...
... must be that F , so by static
typability, the lemma holds.
Intro F and Intro F : and
F , so and F are con-
sistent. By bounded completeness, F
and F . By upward closure,
F F and by static typability,
F ... unifying the representatives of
and , one ( ) for unifying the representatives of
and , and one ( ) for unifying the representatives of
and . Notice that the...
... understanding and written consent of the
subject) [7] and subsequently maintained in BALB/c mice by
intravenous passage every 6 weeks. Promastigotes, obtained
by in vitro transformation of liver and ... was terminated by chilling on ice
and adding 4 lLof0.25
M
PMSF and 60 lLof0.5%(w/v)
egg white trypsin inhibitor in 0.15
M
NaCl. From the
protease treated and untreated...
... constrained analogues of diacylglycerol (DAG). 27.
Modulation of membrane translocation of protein
kinase C (PKC) isozymes a and d by diacylglycerol
Membrane interactions of diacylglycerol derivatives ... initially to
the plasma membrane and only later to the nuclear
membrane and other internal membranes (Fig. 1C and
D, top rows). Additionally, the PKC-a translocatio...
... presently of the nature of the FtsY membrane
interaction in Archaea. Moreover, the steps leading to
deliveryofSRPtothearchaealmembranehaveyettobe
described.
Towards an understanding of the membrane ... soluble and a
membrane- associated protein [11]. While the precise roles
and temporal positions of SRP and FtsY in the bacterial
SRP cycle remain topics of on-going investig...
... glycolipid composition
of the plasma membrane by altering the ganglioside
biosynthetic activity of the cell, while maintaining the
normal process of intracellular transport and membrane
insertion ... changes
of membrane distribution associated with its activation
status.
EGFr and GD3 localization in CHO-K1 cell membranes
Having demonstrated a converse segregation of GD...
... PAGE (15% gel) and analyzed by WB with mAb against GFP
to detect proteolysed fragments of mGFP–calpain 7–Strep. Bands
of full-length WT and C290S are indicated by the arrow, and those
of 30 K are ... bands were detected by the chemiluminescence
method. Membranes were also stained with CBB. Bands of GST–
MIT–pHis and GST–pHis in the pulldown products are indicated by
ar...
... NADPH, and 4 mm NDMA in a 1.0 mL final
volume. The reaction mixture was incubated at 37 °C for
30 min, and the reaction was stopped by the addition of
0.1 mL of 25% zinc sulfate and 0.1 mL of saturated ... concentrations of digitonin.
It is known that low concentrations of digitonin (about
0.05%) selectively damage the outer membrane, and
higher concentrations (about 0.1%)...
... including proliferation, apopto-
sis, differentiation, and so on. Isoforms of numerous
proteins have been studied and compared in this man-
ner, including alternative isoforms of members of the
p53 ... Expression constructs were
generated encoding Myc-tagged versions of these two
isoforms, as well as of the Mxi1-WR isoform that lacks
the SID, and shown to give rise to proteins...