... molar ratio of Ca
2+
⁄ CaM to PhK5
had been reached. The inset shows an expanded view of the indi-
cated area of the spectrum. (Bottom) An overlay of spectra from
the titration of Ca
2+
⁄ CaM ... spectrum indicative
of a large conformational change upon binding of
PhK5 (Fig. 4A) . These changes are observed early in
the titration, at as little as 20% saturation chang...
... (phosphocholine), 165.13 and lipid A- OH (O-deacylated lipid A) , 953.02. Relative
abundance was estimated from the area of molecular ion peak relative to the total area (expressed as percentage). Peaks representing ... H-1 and H-2 (a- configuration). On the
basis of the chemical shift data and the large J
2,3
, J
3,4
and
Table 4. Structures of the Hep4-glycoforms of nont...
... et al.(Eur. J. Biochem. 271) Ó FEBS 2004
Structural characterization of the human Nogo -A functional domains
Solution structure of Nogo-40, a Nogo-66 receptor antagonist enhancing injured
spinal ... ¢-CG
CGCGCGCGGATCCACTGGTACAAAGATTGCT-3¢
(forward) a nd 5¢-CGCGCGCGCCTCGAGCTAAAAT
AAGTCAACTGGTTC-3¢ (reverse). A DNA fragment
encoding human Nogo-66 c...
... evolutionarily one of the most p rimitive
eukaryotic algae. The photosynthetic apparatus of red algae
appears to represent a transitional state between cyanobac-
teria and photosynthetic eukaryo ... ultrastructure of
red algal chloroplasts is similar to t hat of cyanobacteria.
Thylakoid membranes of red algae are not differentiated
into stacked and unstacked regions as fou nd...
... Columbia, Canada
The structure of the antigenic O-chain polysaccharide of
Flavobacterium columnare ATCC 43622, a Gram-negative
bacterium that causes columnaris disease in warm water fish,
was determined ... possible candidates are
LPS, capsule, fimbriae or other appendages of the bacterium,
a hypothesis requiring further investigation.
It is of note that the structure o...
... specific domains suggests that a
detailed characterization of the intrinsic structural and
dynamic characteristics of the individual domains has
the potential to throw valuable light on the interac-
tions ... dextran, molecular mass
2000 kDa also appeared at the same place), and there
was also a small peak seen corresponding to the mono-
mer (Fig. 1A) . This meant t...
... proteases
from a tropical plant, Ervatamia coronaria
Raka Ghosh, Sibani Chakraborty, Chandana Chakrabarti, Jiban Kanti Dattagupta and
Sampa Biswas
Crystallography and Molecular Biology Division, Saha Institute ... studies of the cysteine protease erva-
tamin A from Ervatamia coronaria. Acta Crystallogr F
61, 562–564.
37 Kundu S, Sundd M & Jagannadham MV (2000) Purifi-
cation and c...
... domain X motifs are found at or near the
C-termini of a2 and a3 , respectively. The a- helices a1 ,
a2 and a3 potentially correspond to a- helices aH, aI
and aJ in the thumb of HIV-RT [13].
The domain ... single-stranded DNA substrate, whereas the panel on the right shows cleavage in a 5¢-labeled top-strand, 70 bp
double-stranded DNA substrate. DNA cleavage activity w...
... specific for the
(S)-enantiomers [33,34]. In addition, the nonheme
oxygenase AspH from P. syringae hydroxylates free
l-aspartate, l-aspartate-SNAC, and a linear nonapep-
tide containing an asparagine ... presence
of the two a- helical subdomains in addition to the
catalytic jelly roll fold. Although the presence of these
a- helical subdomains might be an evolutionary relic,...
... requires
the application of multiple analytical approaches [8].
MS has become a powerful analytical tool for the struc-
tural characterization of oligosaccharides. Of several
MS techniques, the soft ... Laboratories
(Mumbai, India). Wheat bran was procured from the local
market. All other chemicals and reagents were of analyti-
cal grade.
Animals, induction of diabetes a...