Báo cáo khóa học: Functional properties of the protein disul

Báo cáo khóa học: Functional properties of the protein disulﬁde oxidoreductase from the archaeon Pyrococcus furiosus A member of a novel protein family related to protein disulﬁde-isomerase doc

... Pedone et al.(Eur. J. Biochem. 271) Ó FEBS 2004 Functional properties of the protein disulfide oxidoreductase from the archaeon Pyrococcus furiosus A member of a novel protein family related to protein ... about protein disulfide oxidoreductases in archaea. A small redox protein w ith a molecular m ass of 12 kDa was purified from the a...

Ngày tải lên : 16/03/2014, 16:20

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Tài liệu Báo cáo khoa học: Functional studies of active-site mutants from Drosophila melanogaster deoxyribonucleoside kinase Investigations of the putative catalytic glutamate–arginine pair and of residues responsible for substrate speciﬁcity docx

... Q81N- fwd:5¢-TGGGCCATGCCCTTT AACAGTTATGTCACG CTG-3¢. Q81N-rev:5¢-CAGCGTGACATAACT GTTAAA GGGCATGGCCCA-3¢. R105H-fwd:5¢-GCTAAAAATAA TGGAGC ACTCCATTTTTAGCGCTCGC-3¢ . R105H- rev:5¢-GCGAGCGCTAAAAATGGAG TGCTCCATTAT TTTTAGC-3¢. ... R105H- rev:5¢-GCGAGCGCTAAAAATGGAG TGCTCCATTAT TTTTAGC-3¢. R105K-fwd:5¢-GCTAAAAATAATGGAG AAATCCATTTTTAGCGCTCGC-3¢. R105K-rev:5¢-GCG AGCGCTAAAAATGGA TTTCTCCATTATTTTTAGC-3¢...

Ngày tải lên : 19/02/2014, 02:20

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Tài liệu Báo cáo khoa học: Functional analysis of the basic helix-loop-helix transcription factor DEC1 in circadian regulation ppt

... 5¢-AAGCT TCACCATGTACCCTGCCCACATGTACCAAGTG TAC-3¢,5¢-AAGCTTCACCATGCCGCACCGG CTC ATCGAGAAAAAGAG-3¢,5¢-AAGCTTCACCATG GCAGTGGTTCTTGAACTTACCTTGAAGC-3¢ or 5¢-AAGCTTCACCATGATTGCCCTGCAGAGTGG TTTACAAGCTG-3¢) ... sets of primers ( 5¢-AAGC TTGAGCGGATCCCCAGCGCGCAACCACC-3¢ and 5¢-GCAGCAGGATCCTCTAGAGAGTTTAGTC TTTG-3¢ for FLAG-DEC1; and 5 ¢-GAATTCGGCGG ACCAGAGAATGGACATTTCCTCAACCATC-3¢ and 5¢-TCTAGACTACAGC...

Ngày tải lên : 19/02/2014, 16:20

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Tài liệu Báo cáo khoa học: Functional dissection of the Schizosaccharomyces pombe Holliday junction resolvase Ydc2: in vivo role in mitochondrial DNA maintenance pptx

... Increasing amounts of total cellular DNA were loaded on the gels, as the hybridization signal in these samples was very low. The general pattern remained the same: the 19.4-kb band was weak and a ... intermediate is therefore crucial for the integrity and maintenance of DNA in all organisms including mitochondrial DNA (mtDNA). Mitochondrial DNA amounts to about 15% of t...

Ngày tải lên : 20/02/2014, 11:20

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Tài liệu Báo cáo khoa học: Functional effects of deleting the coiled-coil motif in Escherichia coli elongation factor Ts pptx

... binds aminoacyl-tRNA (aa-tRNA) and promotes the binding of the aa-tRNA to the A- site of the mRNA-programmed ribosome. Upon codon recognition by a cognate ternary complex (EF-Tu–GTP–aa-tRNA), the ... that the recombinant proteins have the same number of amino acids as the native elongation factors. Activity assays The concentration of EF-Tu active in binding gua...

Ngày tải lên : 21/02/2014, 00:20

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Báo cáo khoa học: Functional analysis of a murine monoclonal antibody against the repetitive region of the fibronectin-binding adhesins fibronectin-binding protein A and fibronectin-binding protein B from Staphylococcus aureus pot

... testing in parallel of up to six different analytes, or up to six different concentrations of the same analyte, over the target surface. Concentrations, ranging from 100 to 1000 nm, of each analyte ... FEBS Monoclonal antibodies against FnBRs of FnBPB A panel of mouse mAbs was produced against the recombinant repetitive region of FnBPB. Analysis of mAbs binding...

Ngày tải lên : 06/03/2014, 22:21

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Báo cáo khoa học: Physicochemical properties and distinct DNA binding capacity of the repressor of temperate Staphylococcus aureus phage /11 doc

... p Bottom O1 O2 O2 cl O3 O2 cro O1 O1 +–– –140 5'CATTTTCTTACCTCCTTAAATTTACCTATAGTATAACCCAATTATTTTTGGTATTCA GTAAAAGAATGGAGGAATTTAAATGGATATCATATTGGGTTAATAAAAACCATAAGT ACAAAAAAATACACGAAAAGCAAACTTTTATGTTGACTCAAGTACACGTATCGTGTAT TGTTTTTTTATGTGCTTTTCGTTTGAAAATACAACTGAGTTCATGTGCATAGCACATA AGTAGGTTTTGTAAGCGGGAGGTGACAACATG TCATCCAAAACATTCGCCCTCCACTGTTGTAC ... Malabika Das*, Amitava Bandhu, Palas K...

Ngày tải lên : 07/03/2014, 00:20

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Báo cáo khoa học: Functional association of the AAA complex and the peroxisomal importomer potx

... 5¢-TTTGCATACCCTCCAAAAGAAAGCGATTATAGTAACATTAATATGCGTACGCTGCAGGTCGAC-3¢ KU231 5¢-ATATATTTACAAATTTACCTATACGCTCTGAGTTGATATTACTTAATCGATGAATTCGAGCTCG-3¢ KU562 5¢-CAGGGCGAAGTAGGTATTAGCCGTTTACATTAGAAAATAAGGTAGCGTACGCTGCAGGTCGAC-3¢ KU699 ... 5¢-CCCCCAGATTGTAGGGTTGCTAAAACTTCTAGCGAGTATACGTACGCTGCAGGTCGAC-3¢ KU1014 5¢-AAATAAGTAGGTAGGGTTTTATAAACTATTCAAATATTTCATCGATGAATTCGAGCTCG-3¢ KU296 5¢-ACCCCGGGTTGAATT...

Ngày tải lên : 07/03/2014, 12:20

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Báo cáo khoa học: Functional characterization of front-end desaturases from trypanosomatids depicts the ﬁrst polyunsaturated fatty acid biosynthetic pathway from a parasitic protozoan ppt

... desaturated by a D4 desaturase to the same ﬁnal 22:5 n-6 and 22:6 n-3 PUFAs. Euglena exhibits another variation in the ﬁrst steps of the pathway. C18 FAs are elongated to C20, and then a D8 desaturase ... cruzi (EAN90580), Euglena gracilis (AAQ19605), Isochrysis galbana (AAV33631), Pavlova lutheri (AAQ98793), Thraustochytrium sp. (AAN75710), Thalassiosira pseu- donana (AAX1450...

Ngày tải lên : 07/03/2014, 12:20

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Báo cáo khóa học: Structural properties of the protein SV-IV potx

... by the mass increase of 42 mass units. The relative level of acetylation of a peptide was estimated on the basis of the intensity ratio of the native and acetylated species. From the data summar- ized ... of the structural organization of SV-IV protein. The amino-acid sequence was analyzed using the BLAST program to ﬁnd similar proteins in the ÔnrÕ dat...

Ngày tải lên : 07/03/2014, 14:20

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