... al. Characterization of sorghum peroxidase
FEBS Journal 273 (2006) 2293–2307 ª 2006 The Authors Journal compilation ª 2006 FEBS 2307
Biochemical characterization of the major sorghum grain
peroxidase
Mamoudou ... which is one of the
most stable plant peroxidases with a T
m
of 90 °Cin
the presence of calcium [38].
In conclusion, the major isoenzyme in s...
... et al. [29], confirm that the
individual domains of the megasynthase may remain
folded despite the lack of the KS dimer.
Characterization of the standalone MAT protein
The LovB MAT active site ... LovB. The
recent crystal structures of the animal FAS showed
the importance of the dimeric KS in stabilizing the
parallel FAS structure [30]. Our results with LovB,...
... to the poor
overall purification. An examination of the elution
fractions of the third chromatographic steps of Mono-
S on SDS ⁄ PAGE showed the major peak of proteins
and the peak fractions of ... format on the
basis of survival in the presence of G418 (500 lgÆmL
)1
;
Sigma, St Louis, MO). The expression and subcellular
localization of rat Kv2.1 of the po...
... 30 °C,
the oxidation of the substrate DHO with the quinone cosub-
strate was coupled to the reduction of the chromogen 2,6-di-
chloroindophenol. The K
m
of DHO was determined by
varying the concentration ... contact between the ubiquinone from the inner
membrane and the active site of DHODH [23,27].
Although there are some differences in processing and
association...
... K
d
is the equilibrium dissociation constant of
the 5¢ exon mimic, h is the fraction of 5¢ exon mimic bound
to the ribozyme, and [ribozyme]
u
is the concentration of
unbound ribozyme in the reaction.
Measurement ... and fitting to the equation:
k
obs
¼ k
1
½Eþk
À1
:
Measurement of the dissociation constant, K
d
P
of the ribozyme–product complex
The equilibrium di...
... 4 °C.
The supernatant was transferred to an HPLC vial. The
products of the reaction were detected at 260 nm using the
HPLC system described above for the synthesis of the CoA-
esters. The assay ... deficiencies of each of the six dif-
ferent steps within the leucine degradation pathway
(Fig. 1) cause their own characteristic disease [1]. The
enzymes of this pathwa...
... kunkelii, the genomes of
the members of the mollicutes group (total number, 7 out of
10) are devoid of genes for the biosynthesis of IPP and
DMAPP. The same is true for the genome of the archaeon
N. ... of the enzyme for that cofactor and the
apparent absence of a redox process as part of the catalytic
cycle. The substrate binding site of the type I...
... products, the cleavage site protected by
the presence of ubiquitin was Ser341, located in the
‘fingers’ region of the catalytic core domain involved
in the recognition of the ubiquitin core. These ... superfamily of
thiol- and metallo proteases specialized in the process-
ing of ubiquitin and ubiquitin-like proteins. They are
responsible for the disassembly of ubiqu...
... element.
Experiments in the presence of calcium were carried out
with the addition of 5 mm CaCl
2
in the buffer. For investi-
gation of the thermal denaturation of annexin B1, the CD
signal at 222 ... Whereas binding of the carbohydrate to
the site on the concave surface of the protein most
likely does not interfere with the essential annexin–
membrane associat...
... members of
the dynamin family, GBPs are classified as a part of
the dynamin superfamily. Although their biochemical
characteristics are well understood, there is only an
incomplete picture of the ... con-
served in the primary structure of the GTPase domain
of hGBP5, so the formation of GMP must be impaired
in an as yet unknown manner. Sequence alignments of
the l...