Báo cáo khoa học: Biochemical characterization of the major sorghum grain peroxidase pptx

Báo cáo khoa học: Biochemical characterization of the major sorghum grain peroxidase pptx

Báo cáo khoa học: Biochemical characterization of the major sorghum grain peroxidase pptx

... al. Characterization of sorghum peroxidase FEBS Journal 273 (2006) 2293–2307 ª 2006 The Authors Journal compilation ª 2006 FEBS 2307 Biochemical characterization of the major sorghum grain peroxidase Mamoudou ... which is one of the most stable plant peroxidases with a T m of 90 °Cin the presence of calcium [38]. In conclusion, the major isoenzyme in s...
Ngày tải lên : 16/03/2014, 13:20
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Báo cáo khoa học: Biochemical characterization of the minimal polyketide synthase domains in the lovastatin nonaketide synthase LovB pot

Báo cáo khoa học: Biochemical characterization of the minimal polyketide synthase domains in the lovastatin nonaketide synthase LovB pot

... et al. [29], confirm that the individual domains of the megasynthase may remain folded despite the lack of the KS dimer. Characterization of the standalone MAT protein The LovB MAT active site ... LovB. The recent crystal structures of the animal FAS showed the importance of the dimeric KS in stabilizing the parallel FAS structure [30]. Our results with LovB,...
Ngày tải lên : 30/03/2014, 08:20
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Báo cáo khoa học: Biochemical characterization of the native Kv2.1 potassium channel ppt

Báo cáo khoa học: Biochemical characterization of the native Kv2.1 potassium channel ppt

... to the poor overall purification. An examination of the elution fractions of the third chromatographic steps of Mono- S on SDS ⁄ PAGE showed the major peak of proteins and the peak fractions of ... format on the basis of survival in the presence of G418 (500 lgÆmL )1 ; Sigma, St Louis, MO). The expression and subcellular localization of rat Kv2.1 of the po...
Ngày tải lên : 30/03/2014, 20:20
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Báo cáo khoa học: Biochemical characterization of recombinant dihydroorotate dehydrogenase from the opportunistic pathogenic yeast Candida albicans pot

Báo cáo khoa học: Biochemical characterization of recombinant dihydroorotate dehydrogenase from the opportunistic pathogenic yeast Candida albicans pot

... 30 °C, the oxidation of the substrate DHO with the quinone cosub- strate was coupled to the reduction of the chromogen 2,6-di- chloroindophenol. The K m of DHO was determined by varying the concentration ... contact between the ubiquinone from the inner membrane and the active site of DHODH [23,27]. Although there are some differences in processing and association...
Ngày tải lên : 07/03/2014, 12:20
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Tài liệu Báo cáo khoa học: Kinetic characterization of the first step of the ribozyme-catalyzed trans excision-splicing reaction docx

Tài liệu Báo cáo khoa học: Kinetic characterization of the first step of the ribozyme-catalyzed trans excision-splicing reaction docx

... K d is the equilibrium dissociation constant of the 5¢ exon mimic, h is the fraction of 5¢ exon mimic bound to the ribozyme, and [ribozyme] u is the concentration of unbound ribozyme in the reaction. Measurement ... and fitting to the equation: k obs ¼ k 1 ½Eþk À1 : Measurement of the dissociation constant, K d P of the ribozyme–product complex The equilibrium di...
Ngày tải lên : 18/02/2014, 18:20
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Tài liệu Báo cáo khoa học: Biochemical characterization of human 3-methylglutaconyl-CoA hydratase and its role in leucine metabolism docx

Tài liệu Báo cáo khoa học: Biochemical characterization of human 3-methylglutaconyl-CoA hydratase and its role in leucine metabolism docx

... 4 °C. The supernatant was transferred to an HPLC vial. The products of the reaction were detected at 260 nm using the HPLC system described above for the synthesis of the CoA- esters. The assay ... deficiencies of each of the six dif- ferent steps within the leucine degradation pathway (Fig. 1) cause their own characteristic disease [1]. The enzymes of this pathwa...
Ngày tải lên : 19/02/2014, 07:20
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Tài liệu Báo cáo khoa học: Biochemical characterization of Bacillus subtilis type II isopentenyl diphosphate isomerase, and phylogenetic distribution of isoprenoid biosynthesis pathways doc

Tài liệu Báo cáo khoa học: Biochemical characterization of Bacillus subtilis type II isopentenyl diphosphate isomerase, and phylogenetic distribution of isoprenoid biosynthesis pathways doc

... kunkelii, the genomes of the members of the mollicutes group (total number, 7 out of 10) are devoid of genes for the biosynthesis of IPP and DMAPP. The same is true for the genome of the archaeon N. ... of the enzyme for that cofactor and the apparent absence of a redox process as part of the catalytic cycle. The substrate binding site of the type I...
Ngày tải lên : 19/02/2014, 13:20
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Báo cáo khoa học: Biochemical characterization of USP7 reveals post-translational modification sites and structural requirements for substrate processing and subcellular localization pptx

Báo cáo khoa học: Biochemical characterization of USP7 reveals post-translational modification sites and structural requirements for substrate processing and subcellular localization pptx

... products, the cleavage site protected by the presence of ubiquitin was Ser341, located in the ‘fingers’ region of the catalytic core domain involved in the recognition of the ubiquitin core. These ... superfamily of thiol- and metallo proteases specialized in the process- ing of ubiquitin and ubiquitin-like proteins. They are responsible for the disassembly of ubiqu...
Ngày tải lên : 07/03/2014, 05:20
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Báo cáo khoa học: Biochemical characterization of annexin B1 from Cysticercus cellulosae pdf

Báo cáo khoa học: Biochemical characterization of annexin B1 from Cysticercus cellulosae pdf

... element. Experiments in the presence of calcium were carried out with the addition of 5 mm CaCl 2 in the buffer. For investi- gation of the thermal denaturation of annexin B1, the CD signal at 222 ... Whereas binding of the carbohydrate to the site on the concave surface of the protein most likely does not interfere with the essential annexin– membrane associat...
Ngày tải lên : 07/03/2014, 12:20
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Báo cáo khoa học: Biochemical properties of the human guanylate binding protein 5 and a tumor-specific truncated splice variant Mark Wehner and Christian Herrmann doc

Báo cáo khoa học: Biochemical properties of the human guanylate binding protein 5 and a tumor-specific truncated splice variant Mark Wehner and Christian Herrmann doc

... members of the dynamin family, GBPs are classified as a part of the dynamin superfamily. Although their biochemical characteristics are well understood, there is only an incomplete picture of the ... con- served in the primary structure of the GTPase domain of hGBP5, so the formation of GMP must be impaired in an as yet unknown manner. Sequence alignments of the l...
Ngày tải lên : 15/03/2014, 10:20
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