... binding
of IN to the processed DNA product. The stability of
IN during the time-course of the reaction and the
influence of protein aggregation are discussed.
Results
General features of the 3¢-end processing ... 3¢-end processing. In the second step,
DNA strand transfer, a pair of processed DNA ends
of the same viral DNA is inserted into the host...
... the logarithm of
the molecular mass of the proteins in the y-axis and the elu-
tion volumes (V
e
) in the x-axis. Comparison of the elution
volumes of the P. abyssi and E. coli ATCases with the
standard ... consequence of
the cooperativity toward aspartate and of the ordered
binding of the substrates, first CP then aspartate [13].
These results raised the...
... K
d
is the equilibrium dissociation constant of
the 5¢ exon mimic, h is the fraction of 5¢ exon mimic bound
to the ribozyme, and [ribozyme]
u
is the concentration of
unbound ribozyme in the reaction.
Measurement ... and fitting to the equation:
k
obs
¼ k
1
½Eþk
À1
:
Measurement of the dissociation constant, K
d
P
of the ribozyme–product complex
The equilibrium di...
... Mg-chelatase of tobacco: identification of a Chl
D cDNA sequence encoding a third subunit, analysis of
the interaction of the three subunits with the yeast two-
hybrid system, and reconstitution of the ... timepoint. The data for the decay of inter-
mediate in the presence of ChlH were fitted to a three-parameter
exponential (k ¼ 31.7 ± 9.5 s
)1
). The evolution o...
... denaturation
of the enzyme. With the exception of the temperature-
activity profile, the characteristics of the enzyme were
determined from initial velocity measurements in the
direction of the NADH-dependent ... in M. thermoautotrophicum [7].
Although several properties were investigated, there has
been no kinetic study of the mechanism of this enzyme.
Aeropyru...
... the
protease.
In the first experiment, we compared the
15
N-HSQC
spectra of WNV NS2B–NS3pro(K96A) in the presence
of 3 and in the presence of the Bz-nKRR-H inhibitor
used in one of the crystal structure ... assignments
have been reported for the WNV NS2B–NS3 protease.
The poor quality of the NMR spectrum of WNV
NS2B–NS3pro in the absence of inhibitors is rem...
... PCR’, using the first PCR product as
template. To make use of the BamHI site in the vector and
the XbaI site in the EhMGL1 gene, the product of the nes-
ted PCR was replaced with the corresponding ... molecular ratio of 1 : 1000,
and further tested for the Met-degrading activity on
the basis of the detection of a-keto acid after the addi-
tion of 2 mm Met (F...
... is the number of features, is a weight for
feature , and estimates the difference between the
two instances’ values at the th feature. The classes
of the
nearest neighbors then determine the ... consists of the val-
ues for all features (the columns) and the function-
chunk code for the focus word. The features de-
scribe the focus word and its local context. Fo...
... those of the substrates used in the kinetic
experiments. The production of p-nitrophenol was linear
with time for the incubation period, and < 5% of the
available substrate was hydrolyzed. The ... these residues stabilize
the distortion of the NAG3 residue. In these interac-
tions, we particularly focused on the Trp664 residue,
which is located at the bottom...
... this study we used IR spectroscopy to study the
secondary structure of the catalytic domain of PKCf
and the effect of its substrate, MgATP, and also to
study the effect of thermal unfolding in the ... structure of 282 residues of a total of 344
have been solved [19]. The catalytic domain of PKCh
possesses a high degree of homology ( 80%) with the
catalyt...