Báo cáo khoa học: Mutational analysis of substrate recogniti

Báo cáo khoa học: Mutational analysis of substrate recognition by human arginase type I ) agmatinase activity of the N130D variant pot

... further corro- borate the existence of active site differences between the isoenzymic forms of human arginase. In fact, whereas partial arginase activity is retained by the N130D variant of arginase ... ligands for the a-carb- oxylate group of the substrate arginine. As this is the Fig. 4. Effects of dialysis against EDTA on the catalytic activity o...

Ngày tải lên : 16/03/2014, 12:20

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Tài liệu Báo cáo khoa học: Mutational analysis of plasminogen activator inhibitor-1 Interactions of a-helix F and its neighbouring structural elements regulates the activity and the rate of latency transition pdf

... proteolysis is involved. PAI-1 belongs to the serine protease inhibitor (serpin) family whose antiproteolytic activity is governed by their structural ﬂexibility. In the active serpin conformation, the reactive ... terms of speciﬁc inhibitory activity (Table 2). Thus, the introduced mutations did not compromise the correct folding of PAI-1 in its active conformation. Com...

Ngày tải lên : 20/02/2014, 11:20

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Tài liệu Báo cáo khoa học: Structural basis for cyclodextrin recognition by Thermoactinomyces vulgaris cyclo⁄maltodextrin-binding protein ppt

... structures of the maltose-maltodextrin-binding protein of the thermo- acidophilic bacterium Alicyclobacillus acidocaldarius provide insight into acid stability of proteins. J Mol Biol 335, 261–274. 23 ... C-domain part of the sugar-binding site. The C-domain part consists of four regions designated region C -I (residues 170–17 7), region C-II (residues 227–23 2), regio...

Ngày tải lên : 19/02/2014, 00:20

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Báo cáo khoa học: A novel ErbB2 epitope targeted by human antitumor immunoagents ppt

... Federico II, Italy 2 CEINGE Biotecnologie avanzate, Napoli, Italy 3 Dipartimento di Chimica Organica e Biochimica, Universita ` di Napoli Federico II, Italy 4 Dipartimento di Chimica, Universita ` di ... ⁄ substrate ratio, temperature and pH conditions in order to maintain the stability of the complex and drive the hydrolysis towards the regions of the protein not involved in...

Ngày tải lên : 06/03/2014, 00:21

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Báo cáo khóa học: Mutational and structural analysis of cobalt-containing nitrile hydratase on substrate and metal binding pdf

... l M , respectively. Therefore, the enzyme exhibited signiﬁcantly stronger inhibition by aromatic organic acids than by small aliphatic organic acids. n-Butyric acid is known not only as a competitive inhibitor ... greater, compared the wild -type enzyme (Table 3). Discussion Substrate speciﬁcity comparison with other NHases The kinetic parameters of P. thermophila NHase, wit...

Ngày tải lên : 16/03/2014, 16:20

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Báo cáo khóa học: Mutational and computational analysis of the role of conserved residues in the active site of a family 18 chitinase docx

... this role, explaining the residual activity of the D215N mutant. The possibility to interact is obviously lost in the D215A mutant which in fact is one of the least active mutants described in ... catalysis in family 18 chitinases proceeds via formation of an oxazolinium ion. This intermediate is formed upon nucleophilic attack of the carbonyl oxygen of the N-acetyl...

Ngày tải lên : 07/03/2014, 14:20

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Tài liệu Báo cáo khoa học: Proteomic analysis of dopamine and a-synuclein interplay in a cellular model of Parkinson’s disease pathogenesis docx

... University of Insubria, Varese, Italy 4 Department of Neuroscience, University of Torino, Italy Introduction Parkinson’s disease (PD) is a sporadic neurodegenera- tive disorder of unknown etiology ... signiﬁcantly affected by a-synuclein overexpression (Fig. S 2). Discussion Proteins differentially expressed in this model are individually linked to PD Most of the identiﬁed pr...

Ngày tải lên : 15/02/2014, 01:20

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Tài liệu Báo cáo khoa học: Kinetic analysis of effector modulation of butyrylcholinesterase-catalysed hydrolysis of acetanilides and homologous esters pdf

... 0, inhibition is competitive; if a = 1, inhibition is noncompetitive; if a > 1, inhibition is mixed. If the ternary complex ESL is nonproductive (b = 0), there is linear mixed inhibition. If ... parabolic competitive inhibition. Parabolic inhibition suggests that the binding of more than one benzalkonium con- tributes to the inhibition (Fig. 4). The multiplicity of cation b...

Ngày tải lên : 18/02/2014, 17:20

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Tài liệu Báo cáo khoa học: Quantitative analysis of the experimental O–J–I–P chlorophyll ﬂuorescence induction kinetics Apparent activation energy and origin of each kinetic step Steve Boisvert, David Joly and Robert Carpentier doc

... DCMU would inhibit completely the activity of PSII by preventing the reduction of the PQ pool [21,22], drastically channging the typical FI trace of thylakoids by eliminating the J–P rise [4,24]. ... reactions involved in the reduction of the acceptor side of PSII [33]. In this simulated model, the J I phase was calculated to be simultaneous with the initial...

Ngày tải lên : 19/02/2014, 05:20

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Tài liệu Báo cáo khóa học: Quantitative analysis, using MALDI-TOF mass spectrometry, of the N-terminal hydrolysis and cyclization reactions of the activation 2 process of onconase pdf

... the matrix and their ionization properties, will not differ signiﬁcantly. Thus, the relative intensities of the different peaks constitute a fair estimate of the fraction of each species in a particular ... group of the Lys9 side-chain, which simultaneously interacts with the main phosphate of the substrate [32]. The closer positioning of the N-terminus to the...

Ngày tải lên : 19/02/2014, 12:20

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