Báo cáo khoa học: Characterization of the bga1-encoded glycoside hydrolase family 35 b-galactosidase of Hypocrea jecorina with galacto-b-D-galactanase activity pdf
... a fit of the phosphorus chemical shifts of
the without ionic, monoionic and diionic forms of the
phosphate group as a function of pH [33,34]. The
phosphorus chemical shift change reflecting the ... site.
Identification of the hydrogen-bonding partners
depends on detailed investigations into the pH depen-
dence of their NMR parameters over the pH range
3–8. Obviously,...
... sequence alignment of Cg-Clp2 with members of the GH18 family. (A) The predicted amino acid sequence of Cg-Clp2 is aligned with the amino acid sequence of three
CLPs from the oyster C. gigas ... characterized
by the degeneration of larval tissues, such as the velum
and the foot, and the remodelling of larval tissues to
produce adult tissues (i.e. the develop...
... of pGL3–basic vector and
was half that of pGL3–promoter vector. These findings
demonstrate the promoter activity of the 5¢-flanking
region of exon 1 in DNASE1. Deletion of the
upstream end of the ... product, of which the 3¢ 380 bp were identical to
exons 1–3 from the 5¢-end of the reverse primer
DN+231 to the 5¢-end of the region where the
sequence of...
... of the first 88
amino acids of PPI1 may participate in the interaction
with the H
+
-ATPase and makes PPI1
588
His
6
a suitable
tool to study the mechanism of action of PPI1.
The analysis of the ... Thus,
all the following experiments were performed with
PPI1
588
His
6
.
The analysis of the effect of PPI1
588
His
6
on the
dependence of PM H
+
-ATPase ac...
... primers. Both of the
genes were constructed either with or without a His-tag at
the 3¢ terminus of the coding region. Thus, the stop codon of
SNO1 and SNZ1 was replaced with bases coding for the
sequence ... complement the defect. The
relationship of all of these genes remains to be clarified.
Expression and purification of Sno1p and Snz1p
In light of the simil...
... unknown but in view of the extensive differences
between the TP isoforms in terms of their mechanisms
of signaling [18,19], modes of regulation⁄ desensitiza-
tion [20–23] and patterns of expression ... element in the regulation of basal Prm3
activity. It is anticipated that the functional characteri-
zation of Prm3 reported herein should provide critical
knowledge of...
... depolymerases. The latter can be
classified into lyases (b-elimination) and hydrolases [2].
All hydrolases involved in degradation of pectin are
classified as members of family 28 of the glycoside
hydrolases, ... moving towards the reducing end
of the substrate, away from the catalytic site. Along
with its exocleaving activity, thereby liberating mono-
galacturonic acid...
... degrees of inhibition were
detected. The most efficient inhibition of the binding of
ICAM-1 L cell transfectants to the I domains was
obtained with the ICAM-1 mAb LB-2 reacting with the
first domain of ... contrast to the CD11b I domain
GST, the majority of the CD11a I domain GST was found
in the insoluble fraction of the E. coli lyzate. Modification
of the...
... factor of
the Ets -family is involved in the regulation. The members of
the Ets -family of transcription factors bind to specific
purine-rich sequences with a core motif of GGAA/T and
control the expression ... mutation in one of the Ets-binding
sequences greatly decreased its promoter activity, suggesting
that the transcription of the a3 integrin gene in these...
... The
occurrence of the C
17
dialdehyde confirmed the novel
site at the C7¢–C8¢ double bond observed with apoly-
copenals and implied the formation of geranial.
Accordingly, the GC-MS analyses of ... the
C5¢–C6¢ and C7¢–C8¢ double bonds depends on the
nature of the substrate.
The cleavage of a sole double bond in the cyclic moi-
ety of 3-OH-c-carotene and of...