... above, these initial conditions were
rapidly conducive to steady conditions where 1 and 3
were present in very similar concentrations, and the
rates of the forward reaction (conversion of 1 to ... 4-phosphate
[28]. The reductive reaction step has been shown to
involve the transfer of a hydride ion from the pro-S
position at C-4 of NADPH to the RE position of C-1
of rea...
... using the authentic sample from the OPS of
Citrobacter PCM 1487 [12] and PCM 1525 [13]. The
absolute
L
configuration of Rha and
D
configuration of
Fuc and ara4dHex were determined by GLC-MS of the
acetylated ... configurations of the monosaccharides
were established by GLC-MS of the acetylated (S)-2-octyl
glycosides [19].
Methylation of the OPS was performed according...
... correlation of sucrose synthesis
and degradation with the process of cold acclimation.
We conclude that acclimation to low temperature rep-
resents a dynamic process, the investigation of which
therefore ... c
A
ðtÞ
In this reaction kinetic, the reaction rate r
AfiB
(t) depends
on the substrate concentration c
A
(t) and the rate constant k.
The model code and a detailed des...
... structure of the C2-SurE to a resolution of
2.7 A
˚
. In both crystal forms the electron density
accounted for the majority of the residues. There were
no disordered regions in the map. The quality of
refinement ... formed by residues 3 4–5 0 under-
goes conformational change from a ‘closed’ to an
‘open’ form. The shift in the position of the loop away
from the...
... structure-activity studies. The high
selectivity of the a-conotoxins, together with the possibility
of obtaining detailed information on their three dimen-
sional structures and the relative ease of synthesis, ... different
systems is therefore essential to prove the utility of the
oocyte system and the validity of correlations based on the
characterization of...
... are the result of one trial
but are representative of n ¼ 4 determinations from separate pre-
parations of enzyme.
Table 3. The effect of inorganic phosphate concentration on Mg.ATP kinetics of ... as
the data set was typical of noncompetitive inhibition.
Braxton et al. [17] previously defined the effect of an
allosteric ligand on V
max
via the use of Arrhenius pl...
... association constant of SNase( 1–1 26)
bound to pdTp in the presence of Ca
2+
is approxi-
mately threefold lower than that of the wild-type pro-
tein. Therefore, some or all of the residues in the 12 7–
149 ... secondary
structure only random coil. We can therefore postulate
that, when the segment beyond W140 was removed, the
secondary structure of the protein dis...
... chemical chaperones and modu-
lation of the expression conditions to reduce the rate
of protein biosynthesis [9,3 5–3 7], whereas in the second
major efforts are devoted to optimizing the refolding
process ... concerns aggregation propensity.
Reports on the upregulation of the quality control sys-
tem upon the accumulation of misfolded proteins in
the cytoplasm s...
... cell-cycle regulation
The mode of action of RMI1 in the regulation of
energy homeostasis might be based on the regulation of
adipocyte hyperplasia. Studies have shown that siRNA
knockdown of RMI1 resulted ... 567
roles in the control of energy metabolism, leading to
body weight reduction. Further investigations to clarify
the each contribution of adipocyte and cent...
... 505 1–5 060 ª 2010 The Authors Journal compilation ª 2010 FEBS 5051
precise molecular mechanisms underlying the transcrip-
tional control of PP2A genes and the effects of diverse
combinations of ... site. Furthermore, the putative pro-
moter region of the Ppp2r5c gene contained binding
sites for MZF1, CREB, GATA1, Hsf1 ⁄ 2 and CdxA.
A
B
Fig. 1. A conserved region of the...