Báo cáo khoa học: Structural flexibility of the methanogenic-type seryl-tRNA synthetase active site and its implication for specific substrate recognition pptx
... FEBS
Structural flexibility of the methanogenic-type seryl-tRNA
synthetase active site and its implication for specific
substrate recognition
Silvija Bilokapic
1
, Jasmina Rokov Plavec
1
, Nenad Ban
2
and ... consists
of a long a-helical arm, and extensions in the N- and
C-termini of the enzyme ensure recognition of two
unusual mitochondrial t...
... Asn51 and Cys54 (at the
N-cap of the second helix); Ala64 and Ala65 (in the
b-turn between the second and third helices); and Thr72
and Ala73 (at the C-cap of the third helix) (Fig. 4A).
The first ... in the monomeric structure of CAC,
and (b) the inherent flexibility for the majority of the
residues. Thus, the conformational entropy appears to...
... estimated that the a-helix
content is 24% in the monomeric form and 45% in the
trimeric form, and b-structure is absent from both forms.
The CD spectra of the 1–70 and 70–90 regions of the protein
are ... in the trimeric
form is due to subunit association and the consequent loss
of exposed surface. Arg57 and Arg81 may be located in the
region of interaction...
... study the
secondary structure of the catalytic domain of PKCf
and the effect of its substrate, MgATP, and also to
study the effect of thermal unfolding in the presence
and the absence of MgATP. ... esti-
mation of the bandwidth and the intensity of the bands
making up the amide I or I¢ region. Thereafter, curve fitting
was performed and the heig...
... Zn
2+
.
Furthermore, the d etermination of the Nogo-40 solution
structure offers a starting point for further understanding
the interaction between NgR and Nogo-40, and for
future designs of molecules ... clue for
future functional studies of Nog o-A.
On the other hand, the recent identification of Nogo-40
as a potent NgR antagonist suggests a promising starting
poi...
... and
2-amino-2,6-dideoxygalactose, the
1
H-NMR spectra of
which were identical with those of authentic reference
glycoses, and further confirms their characterization. The
combined MS data and the isolation of the two aminoglyc-
oses ... spectra and the characterization of the glycose
components in the O-PS were determined from the appli-
cation of COSY, TOCSY,...
... oligomers
Because of the large size of the core of the oligomer,
the NMR spectra do not show any signals from the
interior of the core and thus do not give any informa-
tion on its structural details. Nevertheless, ... structural and
dynamic characteristics of the individual domains has
the potential to throw valuable light on the interac-
tions of the...
... repeating units.
Fig. 4. CE-ES-MS analysis (+ ion mode) of
LPS (A) and carboxyl-reduced LPS (B) of
A. salmonicida. The inset sho ws the ratio of
the native and amidated forms of LPS and
CPS. Separation ... 2-acetamido-2-deoxy-
D
-galacturonic acid. The
sequence of constituent glycoses and the location of
L
-Ala
were confirmed by 2D NMR analysis performed on both
C...
... essential for the thermal stability a nd
biological functions of the bacteria i n extreme environments
[9–11]. The polar lipids found in Thermus aquaticus,
Thermus filiformis, Thermus scotoductus,andThermus ... study is
the first to determine the full structure of glycoglycerolipid
in thermophilic bacteria Meiothermus spp. The structural
information will be very useful for...
... 6B).
Altogether, these data confirm the presence of sul-
fated high-mannose type oligosaccharides in cruzipain
and in its C-terminal domain. This is the first report of
the use of nor-harmane as matrix for ... or from the whole protein) and using
different equipment. Therefore, the retention of water
can be explained taking into account its strong interac-
tion with...