... 2008)
doi :10 .11 11/ j .17 42-4658.2008.06372.x
Mammalian target of rapamycin complex 1 (mTORC1) phosphorylates
proteins such as eukaryotic initiation factor 4E-binding protein 1 (4E-BP1)
and the S6 kinases. ... of initiation
factor 4E-binding protein 1 in vivo. J Biol Chem 278,
40 717 –40722.
20 Wang X & Proud CG (2006) The mTOR pathway in
the contr...
... revealed that these three proteins dif-
fused with diffusion constants of 11 .7, 12 .9 and
5.4 lm
2
Æs
)1
, respectively, in the part of the cytoplasm
outside of the granules. FRAP analysis in the gran-
ules ... from
fluctuations of probes provides the diffusional proper-
ties of proteins [13 ] and binding interactions [14 ].
FRAP is a conventional technique used...
... YXXXK),
RDH2
1) 137
(oligonucleotides 10 14 ; amino acids 1 13 7,
truncated after the GLVNNAG region), and RDH2
1) 58
(oligonucleotides 10 15 ; amino acids 1 58, truncated just
after the cofactor-binding sequence ... activity
[12 ]. The specific activity of each amphioxus enzyme
was 0.25 nmol of retinolÆmin
)1
Æ(mg of microsomes)
)1
for RDH1 incubated with 15 lm all-tra...
... 0.5% Triton X -10 0,
10 lgÆmL
)1
aprotinin). After determination of the protein
concentration, cell lysate containing 10 0 lgofproteinwas
diluted to 300 lL with lysis buffer, added 15 lLof
immobilized ... the cDNA encoding
human P-LAP [3]. Analysis of the cDNA indicates that the
enzyme is a type II membrane-spanning protein, which
belongs to the M1 family of zinc-met...
... the accessi-
bility of the active site was better in the protein bear-
ing the N2 domain than in the protein bearing the Nc
domain. Thus, the accessibility of the active site in the
N2 proteins ... the protein in
the presence of the water–lipid interface. Also, both
the N-terminal and C-terminal domains of hoPL were
involved in the inactivat...
... 607
213 49
1. 1
36.9
10 87.3
759.9
B
16 2 16 8
2 910
14 854
18 .0
88.4
313 5.5
836.4
C
16 2
16 6
3435
16 166
21. 2 97.4
3228.9
837.7
estimated. In the second approach, on the other
hand, as described in the ... con-
structed from the result of cross-language retrieval
of relevant news articles in section 3 .1. Referring
to the definition of the set D of r...
... combination of
a potent quinolone combined with clindamycin. This strategy
is commonly followed in our facility for infection of unknown
origin and generally corresponds to the guidelines of the ... decompression [12 ]; (ii) increasing the vascularity of
the wound, thus improving cutaneous perfusion [13 ,14 ]; (iii)
promoting granulation tissue formation [15 ,16 ]; and/or (i...
... 4490
Tel: + 81 29 853 5600 (5466)
E-mail: yokokawa@ims.tsukuba.ac.jp
(Received 9 March 2 011 , revised 24 May
2 011 , accepted 16 June 2 011 )
doi :10 .11 11/ j .17 42-4658.2 011 .08222.x
Studies of ion pumps, ... ion
transport reaction [14 ,16 19 ].
The single line scanning method [8 ,15 ], in which an
AFM probe repeatedly scanned on a single line
(along the y-axis direction in F...