Báo cáo khoa học: Contributions to catalysis and potential i

Báo cáo khoa học: Contributions to catalysis and potential interactions of the three catalytic domains in a contiguous trimeric creatine kinase doc

... Contributions to catalysis and potential interactions of the three catalytic domains in a contiguous trimeric creatine kinase Gregg G. Hoffman 1 , Omar Davulcu 2 , Sona Sona 1 and W. Ross ... residue(s) to selectively eliminate catalysis in each of the individual domains and in all possible combinations of domains. Inactivation of this...

Ngày tải lên : 16/03/2014, 06:20

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Báo cáo khoa học: Par j 1 and Par j 2, the two major allergens in Parietaria judaica, bind preferentially to monoacylated negative lipids potx

... Gonza ´ lez-Rioja R, Ferrer A, Arilla MC, Ibarrola I, Viguera AR, Andreu C, Martı ´ nez A & Asturias JA (2007) Diagnosis of Parietaria judaica pollen allergy using natural and recombinant Par ... protein as the major protein in the surface wax of broccoli (Brassica oleracea) leaves. Arch Biochem Biophys 331, 460–468. 48 Takishima K, Watanabe S, Yama da M, Suga T & Mamiy...

Ngày tải lên : 30/03/2014, 02:20

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Báo cáo khoa học: Substrate speciﬁcity and transport mode of the proton-dependent amino acid transporter mPAT2 potx

... substrate spectrum that includes not only t he amino acids glycine, alanine, serine and proline but also osmolytes such as sarcosine and betaine, and the D -enantiomers of serine and alanine. The apparent ... the apparent K m values of glycine and a lanine at pH values of pH 5.5–8.5 (Fig. 4) and a broad range of membrane potentials. At hyperpolarized membrane pot...

Ngày tải lên : 07/03/2014, 16:20

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Báo cáo khoa học: Dual mitochondrial localization and different roles of the reversible reaction of mammalian ferrochelatase ppt

... Shimokawa O, Matsui H & Taketani S (2005) Mechanisms involved in delta-aminolevulinic acid (ALA)-induced photosensitivity of tumor cells: relation of ferrochelatase and uptake of ALA to the accumulation ... trypsin. The amount of ferrochelatase in the mitochondria was decreased by trypsin treatment, suggesting that a part of the ferrochelatase protein is loca...

Ngày tải lên : 16/03/2014, 00:20

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Báo cáo khoa học: nNOS inhibition, antimicrobial and anticancer activity of the amphibian skin peptide, citropin 1.1 and synthetic modiﬁcations pot

... reactive radical NO [21,22]. Nitric oxide synthases are composed of two domains: (a) the catalytic oxygenase domain that binds heme, tetrahydrobiopterin and the substrate arginine, and (b) the ... supplying reductase domain that binds NADPH, FAD and FMN. Communi- cation between the oxygenase and reductase domains is determined by the regulatory protein calmodulin whic...

Ngày tải lên : 17/03/2014, 09:20

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Báo cáo khoa học: Total chemical synthesis and NMR characterization of the glycopeptide tx5a, a heavily post-translationally modiﬁed conotoxin, reveals that the glycan structure is a-D-Gal-(1ﬁ3)-a-D-GalNAc pot

... of N-acetylgalactosamine ( GalNAc) and galactose (Gal), but the interresidue linkage was not characterized. W e h ypothesized that t x 5a contained the T-antigen, b- D -Gal-(1ﬁ3) -a- D -GalNAc, ... on the t hreonine residue of tx 5a was previously investigated [9]. It was shown t hat the peptide contained N-acetylgalactosamine (GalNAc) and galactose ( Gal) in approximatel...

Ngày tải lên : 23/03/2014, 13:20

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Báo cáo khoa học: Site-directed mutagenesis and footprinting analysis of the interaction of the sunﬂower KNOX protein HAKN1 with DNA ppt

... USA). Ampliﬁcations were performed using Pfu DNA polymerase and oligonucleotides MALN1: 5¢-GC GGAATTCAAAAAGAGAAAGAAAGGG-3¢ and MALC: 5¢-GGCCTGCAGCTAGAGAAGTGAAACATC-3¢ with HAKN1 cDNA [28] as the ... radical footprinting and interference assays A more detailed picture of the binding of HAKN1 to its target site was obtained by the analysis of footprinting patterns after...

Ngày tải lên : 23/03/2014, 13:20

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Báo cáo khoa học: Phosphatidylserine induces functional and structural alterations of the membrane-associated pleckstrin homology domain of phospholipase C-d1 docx

... 187 Phosphatidylserine induces functional and structural alterations of the membrane-associated pleckstrin homology domain of phospholipase C-d1 Naoko Uekama, Takio Sugita, Masashi Okada, Hitoshi Yagisawa and ... diffraction. As shown with dotted lines, the chemical shifts of the sig- nals of Ala116 and Ala118 are not affected by addition of PS, indicating that conformat...

Ngày tải lên : 30/03/2014, 10:20

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Báo cáo khoa học: Comparative metal binding and genomic analysis of the avian (chicken) and mammalian metallothionein potx

... (chicken) and mammalian metallothionein Laura Villarreal 1 , Laura Tı ´o 2 , Merce ` Capdevila 1 and Sı ´lvia Atrian 2 1 Departament de Quı ´ mica, Facultat de Cie ` ncies, Universitat Auto ` noma de ... involving a simultaneous intermolecular formation (N a1 –Cd a2 and N a2 –Cd a1 ) and intramolecu- lar loss (N a1 –Cd a1 and N a2 –Cd a2 ) of two Cd–NHis bonds, via...

Ngày tải lên : 30/03/2014, 11:20

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Báo cáo khoa học: Structure, mRNA expression and linkage mapping of the brain-type fatty acid-binding protein gene (fabp7 ) from zebraﬁsh (Danio rerio) potx

... 0.915 tcaCCAAttga V$ECAT/NFY.01 nuclear factor Y )1203 (+) 1.000 0.937 aggacCCAAtaaggga V$GATA/GATA2.02 GATA-binding factor 2 )177 (–) 1.000 0.912 agcGATAtta V$GATA/GATA1.03 GATA-binding factor 1 ... taaaGATAaacaa V$GATA/GATA1.02 GATA-binding factor 1 )940 (+) 1.000 0.965 taagaGATAatcgg V$SORY/SOX5.01 Sox-5 )200 (–) 1.000 0.860 attaCAATtg V$SORY/SOX5.01 Sox-5 )561 (–) 1.000 0.989 caaaCAATgc V...

Ngày tải lên : 31/03/2014, 07:20

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