... claim to original US government works
Mutational analyses of human eIF5A- 1 – identification
of amino acid residues critical for eIF5A activity and
hypusine modification
Veridiana S. P. Cano
1,2,
*, ... struc-
tures of both the N-terminal and C-terminal domains
of eIF5A (Fig. 1, amino acids 1 7–8 2 and 8 5–1 46) are
necessary for the biol...
... products for discovering active compounds and target
information. Curr. Medical Chem. 10, 232 7–2 342.
Ó FEBS 2003 Binding analyses of human PPARc–LBD to ligands (Eur. J. Biochem. 271) 397
Binding analyses ... energies of the ligands binding to PPARc. The binding free energies (kcalÆmol
)1
) of the protein–ligand complex were
estimated by the scoring function of
AUTODOCK
3...
... cleft. The arginase loop (residues 12 6–1 43 in
the sequence of human liver arginase) contains Asn130
and other residues proposed as ligands for the a-carb-
oxylate group of the substrate arginine. ... shown in Table 1, upon mutation of Asn130 to
aspartate, the arginase activity of human arginase I was
reduced to about 17% of the wild-type activity, and the
K
m
val...
... 172
Consensus – G K I – D
E
LL
V
– – V
L
I
D––T
PAI-1 K G M I S N L L G K G A V D Q L T
a
1
PI QGKI VDLVK– – ELDRDT
ATIII E G R I T D V I P S E A I N E L T
Ó FEBS 2003 Mutational analysis of PAI-1 ... substitution of the residues F100, V126, F128
and I137, respectively, led to increased substrate beha-
viour and a low unstable inhibitory activity, and for
I13...
... H. (1993) Identification of glutamic acid
204 and aspartic acid 200 in chitinase A1 of Bacillus circulans WL-
12 as essential residues for chitinase activity. J. Biol. Chem. 268,
1856 7–1 8572.
27. ... being formed, and covalent bonds being formed
and broken). Thus, the calculated effect of rotation of
Asp142 on the pK
a
of Glu144 only gives an indication of
what...
... 45
Nicotinonitrile
k
cat
(s
)1
) 131 – – – –
K
m
(mM) 0.12 – – – –
k
cat
/K
m
(s
)1
Æm
M
)1
) 1090 – – – –
Isonicotinonitrile
k
cat
(s
)1
)9 0– –
K
m
(m
M
) 0.079 – – – –
k
cat
/K
m
(s
)1
Æm
M
)1
) 1140 – – – –
Cobalt content
Co/ab ... various inhibitor concen-
trations ( 0–1 0 m
M
for n-butyric acid, 0–5 0 m
M
for
prop...
... [19], and NMR
spectroscopic data of p25a homologs from mice,
Caenorhabditis elegans and humans [2 0–2 2] demon-
strate a folded central core and unfolded N- and C-ter-
minal extensions of 45 and ... [28]. The instability of
DA at the neutral pH of the cytosol causes the forma-
tion of toxic aminochromes, and these species are not
formed when DA is protonated at the a...
... Positions
of E665 and E555 of the CBP–KIX domain, and residues K291 and R294 of the c-Myb transactivation domain are indicated. (C) The
CBP–KIX:cMyb:MLL1 TAD ternary complex (drawn form PDB code: ... TAD is shown in green and the colors for
the CBP–KIX:cMyb are as in (B). Upon formation of the ternary complex, residues E665 and E666 of the CBP–KIX domain become order...