Báo cáo khoa học: Mutational analyses of human eIF5A-1 – identification of amino acid residues critical for eIF5A activity and hypusine modification doc

Báo cáo khoa học: Mutational analyses of human eIF5A-1 – identification of amino acid residues critical for eIF5A activity and hypusine modification doc

Báo cáo khoa học: Mutational analyses of human eIF5A-1 – identification of amino acid residues critical for eIF5A activity and hypusine modification doc

... claim to original US government works Mutational analyses of human eIF5A- 1 – identification of amino acid residues critical for eIF5A activity and hypusine modification Veridiana S. P. Cano 1,2, *, ... struc- tures of both the N-terminal and C-terminal domains of eIF5A (Fig. 1, amino acids 1 7–8 2 and 8 5–1 46) are necessary for the biol...
Ngày tải lên : 16/03/2014, 06:20
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Báo cáo khoa học: Insulin resistance in human adipocytes occurs downstream of IRS1 after surgical cell isolation but at the 1 level of phosphorylation of IRS1 in type 2 diabetes pot

Báo cáo khoa học: Insulin resistance in human adipocytes occurs downstream of IRS1 after surgical cell isolation but at the 1 level of phosphorylation of IRS1 in type 2 diabetes pot

... 1. 1–1 .8 1. 1–1 .8 IRS1 0. 6–0 .7 0. 6–0 .7 1. 8–2 .0 1. 8–2 .0 1. 8–2 .0 1. 8–2 .0 PKB 0. 9–1 .1 0. 3–0 .4 0. 6–0 .7 0. 6–0 .7 0. 6–0 .7 0. 6–0 .7 Glucose transport 0. 1–0 .2 0.0 2–0 .03 0. 1–0 .2 0. 1–0 .2 0. 1–0 .2 0. 1–0 .2 A. Danielsson ... (MAP) kinases – extracellular signal-related kinase (ERK) 1 and 2 [3], and p38 [4,5] – protein k...
Ngày tải lên : 07/03/2014, 16:20
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Báo cáo khóa học: Binding analyses between Human PPARc–LBD and ligands Surface plasmon resonance biosensor assay correlating with circular dichroic spectroscopy determination and molecular docking ppt

Báo cáo khóa học: Binding analyses between Human PPARc–LBD and ligands Surface plasmon resonance biosensor assay correlating with circular dichroic spectroscopy determination and molecular docking ppt

... products for discovering active compounds and target information. Curr. Medical Chem. 10, 232 7–2 342. Ó FEBS 2003 Binding analyses of human PPARc–LBD to ligands (Eur. J. Biochem. 271) 397 Binding analyses ... energies of the ligands binding to PPARc. The binding free energies (kcalÆmol )1 ) of the protein–ligand complex were estimated by the scoring function of AUTODOCK 3...
Ngày tải lên : 16/03/2014, 16:20
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Báo cáo khoa học: Mutational analysis of substrate recognition by human arginase type I ) agmatinase activity of the N130D variant pot

Báo cáo khoa học: Mutational analysis of substrate recognition by human arginase type I ) agmatinase activity of the N130D variant pot

... cleft. The arginase loop (residues 12 6–1 43 in the sequence of human liver arginase) contains Asn130 and other residues proposed as ligands for the a-carb- oxylate group of the substrate arginine. ... shown in Table 1, upon mutation of Asn130 to aspartate, the arginase activity of human arginase I was reduced to about 17% of the wild-type activity, and the K m val...
Ngày tải lên : 16/03/2014, 12:20
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Tài liệu Báo cáo khoa học: Mutational analysis of plasminogen activator inhibitor-1 Interactions of a-helix F and its neighbouring structural elements regulates the activity and the rate of latency transition pdf

Tài liệu Báo cáo khoa học: Mutational analysis of plasminogen activator inhibitor-1 Interactions of a-helix F and its neighbouring structural elements regulates the activity and the rate of latency transition pdf

... 172 Consensus – G K I – D E LL V – – V L I D––T PAI-1 K G M I S N L L G K G A V D Q L T a 1 PI QGKI VDLVK– – ELDRDT ATIII E G R I T D V I P S E A I N E L T Ó FEBS 2003 Mutational analysis of PAI-1 ... substitution of the residues F100, V126, F128 and I137, respectively, led to increased substrate beha- viour and a low unstable inhibitory activity, and for I13...
Ngày tải lên : 20/02/2014, 11:20
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Báo cáo khóa học: Mutational and computational analysis of the role of conserved residues in the active site of a family 18 chitinase docx

Báo cáo khóa học: Mutational and computational analysis of the role of conserved residues in the active site of a family 18 chitinase docx

... H. (1993) Identification of glutamic acid 204 and aspartic acid 200 in chitinase A1 of Bacillus circulans WL- 12 as essential residues for chitinase activity. J. Biol. Chem. 268, 1856 7–1 8572. 27. ... being formed, and covalent bonds being formed and broken). Thus, the calculated effect of rotation of Asp142 on the pK a of Glu144 only gives an indication of what...
Ngày tải lên : 07/03/2014, 14:20
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Báo cáo khóa học: Mutational and structural analysis of cobalt-containing nitrile hydratase on substrate and metal binding pdf

Báo cáo khóa học: Mutational and structural analysis of cobalt-containing nitrile hydratase on substrate and metal binding pdf

... 45 Nicotinonitrile k cat (s )1 ) 131 – – – – K m (mM) 0.12 – – – – k cat /K m (s )1 Æm M )1 ) 1090 – – – – Isonicotinonitrile k cat (s )1 )9 0– – K m (m M ) 0.079 – – – – k cat /K m (s )1 Æm M )1 ) 1140 – – – – Cobalt content Co/ab ... various inhibitor concen- trations ( 0–1 0 m M for n-butyric acid, 0–5 0 m M for prop...
Ngày tải lên : 16/03/2014, 16:20
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Tài liệu Báo cáo khoa học: P25a ⁄ TPPP expression increases plasma membrane presentation of the dopamine transporter and enhances cellular sensitivity to dopamine toxicity pptx

Tài liệu Báo cáo khoa học: P25a ⁄ TPPP expression increases plasma membrane presentation of the dopamine transporter and enhances cellular sensitivity to dopamine toxicity pptx

... [19], and NMR spectroscopic data of p25a homologs from mice, Caenorhabditis elegans and humans [2 0–2 2] demon- strate a folded central core and unfolded N- and C-ter- minal extensions of  45 and ... [28]. The instability of DA at the neutral pH of the cytosol causes the forma- tion of toxic aminochromes, and these species are not formed when DA is protonated at the a...
Ngày tải lên : 14/02/2014, 21:20
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Tài liệu Báo cáo khoa học: Mixed lineage leukemia: a structure–function perspective of the MLL1 protein ppt

Tài liệu Báo cáo khoa học: Mixed lineage leukemia: a structure–function perspective of the MLL1 protein ppt

... Positions of E665 and E555 of the CBP–KIX domain, and residues K291 and R294 of the c-Myb transactivation domain are indicated. (C) The CBP–KIX:cMyb:MLL1 TAD ternary complex (drawn form PDB code: ... TAD is shown in green and the colors for the CBP–KIX:cMyb are as in (B). Upon formation of the ternary complex, residues E665 and E666 of the CBP–KIX domain become order...
Ngày tải lên : 16/02/2014, 14:20
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