... has a dual effect on HIF-1 activity under
normoxic and hypoxic conditions, demonstrating that
this molecule cannot be used in cancer treatment. In
the context of cancer treatment, the use of this ... basal and PMA-stimulated
c-myc transcriptional activity (Fig. 2C). AP-1 activity
was also low in unstimulated HepG2 cells. PMA
enhanced this activity and, under n...
... native-like conformations.
Conclusions
The comparison of apo- and holo-lMb by MD simu-
lations reveals a dramatic effect of the heme cofactor,
which is seen to stabilize native Mb-like folded confor-
mations ... affects
protein conformation and dynamics; and (c) to probe
the transitions of each individual helix during the ini-
tial step of thermal unfolding.
Results and Disc...
... first few sec-
onds of the reaction under conditions when at least
90% of the activity was retained; however, these are
clearly not ideal conditions. With NADH as cofactor,
the pK on the ‘less ... the effect
of pH on the kinetic parameters of a two-substrate
enzyme, one substrate is held at 10 · K
m
(91% saturat-
ing) and the variation of initial rate with the concen-...
... experiment, conclusions regarding
the effects of the additives on the chaperone activity of
aB-crystallin were drawn based on the measured percentage
protection when both the additive and chaperone were
present ... In
addition, for each assay we used concentrations of
aB-crystallin that only partially inhibited aggregation
of the target protein in order to enable the effects...
... different effects on the
activity of RTA. Whereas, one had no effect on the activity
of the enzyme (R258A), others reduced activity by one
(R213A, R258D, R48A) or by two (R213D) orders of
N122A
Y80
N122A
Y80
Fig. ... expression levels and, as such, these mutants
could not be purified. To assess the effect of substitutions
made at each of the arginyl residues on catal...
... attentional
salience. One determiner of whether attentional
or propositional effects are dominant is the type of
information provided by the accented constituent.
Because nonpronominals contribute ... shifts.
When intonation and centering collide
My synthesis of the claims in PH90 and GJW89 pro-
duces an attentional interpretation of pitch accents,
modeled by operations o...
... plactin dually modulates prothrombin
activation, depending on the conditions of catalysis by
Xa. Under conditions where membrane-associated Xa
formation is restricted, plactin enhances the formation
of ... (B) and 0.5 nM in the other panels. Inset shows the effect of plactin D on factor Xa activity in each con-
dition. The Xa concentration was 0.5 n
M for all incubations...
... ligand
under alkaline conditions and in the presence of
GdmHCl is different. Under alkaline conditions release
of the axial ligand is postulated to arise from the
deprotonation of an ionizable ... amounts
of guanidinium hydrochloride (GdmHCl) the protein
unfolds resulting in the perturbation of a number of
electronic absorption bands and the appearance in the
spectrum...
... combination of
curcumin and capsaicin. Thus, the protective effect of
the combination of dietary curcumin and capsaicin on
LDL oxidation both in vivo and in vitro was greater
than that of the individual ... and
conjugated dienes [51]. In experimental animals, iron
overload can be effected by intraperitoneal injection of
iron salts [51].
Effects of dietary curcumin, cap...
... the
region of DNA strand breaks, and consequent forma-
tion of MRN foci in cells exposed to IR, led to the
hypothesis that the MRN complex functions in the
sensing of DNA strand breakage and in ... the
importance of DNA strand breaks in ATM activation
in vitro, suggested the possibility that the status of
ATM or cofactors might determine the effect of DNA
strand ends on AT...